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    Polyhydroxyalkanoate, method for production thereof and microorganisms for use in the same
    1.
    发明授权
    Polyhydroxyalkanoate, method for production thereof and microorganisms for use in the same 失效
    聚羟基链烷酸酯，其制备方法和用于其的微生物

    公开(公告)号：US06586562B2

    公开(公告)日：2003-07-01

    申请号：US09748205

    申请日：2000-12-27

    申请人： Tsutomu Honma ,  Toyoko Kobayashi ,  Tetsuya Yano ,  Shin Kobayashi ,  Takeshi Imamura ,  Sakae Suda ,  Takashi Kenmoku

    发明人： Tsutomu Honma ,  Toyoko Kobayashi ,  Tetsuya Yano ,  Shin Kobayashi ,  Takeshi Imamura ,  Sakae Suda ,  Takashi Kenmoku

    IPC分类号： C08G6306

    CPC分类号： C12P7/62 ,  C08G63/06 ,  C08G63/6822 ,  C08G63/6852 ,  Y10S435/874

    摘要： A polyhydroxyalkanoate having a monomer unit composition represented by General Formula (1): AmB(1−m)  (1) wherein A is represented by General Formula (2), B is at least one selected from the group consisting of monomer units represented by General Formula (3) or (4), and m has a value of 0.01 or larger and smaller than 1:  wherein n has a value of 0 to 10, k has a value of 3 or 5, and R is at least one group selected from the group consisting of groups represented by General Formulae (5) to (7):  in Formula (5) R1 is a group selected from the group consisting of a hydrogen atom (H), halogen atoms, —CN, —NO2, —CF3, —C2F5 and —C3F7; and q is an integer selected from 1 to 8;  in Formula (6) R2 is a group selected from the group consisting of a hydrogen atom (H), halogen atoms, —CN, —NO2, —CF3, —C2F5 and —C3F7; and r is an integer selected from 1 to 8;  in Formula (7) R3 is a group selected from the group consisting of a hydrogen atom (H), halogen atoms, —CN, —NO2, —CF3, —C2F5 and —C3F7; and s is an integer selected from 1 to 8. The efficient production methods are also provided.

    摘要翻译： 具有由通式（1）表示的单体单元组成的聚羟基链烷酸酯：其中A由通式（2）表示，B为选自由通式（3）或（4）表示的单体单元中的至少一种， ，m的值为0.01以上且小于1，其中n为0〜10，k为3或5，R为选自由通式 （5）〜（7）中，式（5）中，R 1为选自氢原子（H），卤素原子，-CN，-NO 2，-CF 3，-C 2 F 5和-C 3 F 7的基团。 q为选自1至8的整数; 在式（6）中，R 2是选自氢原子（H），卤素原子，-CN，-NO 2，-CF 3，-C 2 F 5和-C 3 F 7的基团; 并且r是选自1至8的整数; 在式（7）中，R 3是选自氢原子（H），卤素原子，-CN，-NO 2，-CF 3，-C 2 F 5和-C 3 F 7的基团; s是选自1至8的整数。还提供了有效的制备方法。

    Polyhydroxyalkanoate, method for production thereof and microorganisms for use in the same
    2.
    发明授权
    Polyhydroxyalkanoate, method for production thereof and microorganisms for use in the same 失效
    聚羟基链烷酸酯，其制备方法和用于其的微生物

    公开(公告)号：US07169598B2

    公开(公告)日：2007-01-30

    申请号：US10649646

    申请日：2003-08-28

    申请人： Tsutomu Honma ,  Toyoko Kobayashi ,  Tetsuya Yano ,  Shin Kobayashi ,  Takeshi Imamura ,  Sakae Suda ,  Takashi Kenmoku

    发明人： Tsutomu Honma ,  Toyoko Kobayashi ,  Tetsuya Yano ,  Shin Kobayashi ,  Takeshi Imamura ,  Sakae Suda ,  Takashi Kenmoku

    IPC分类号： C12N1/20

    CPC分类号： C12P7/62 ,  C08G63/06 ,  C08G63/6822 ,  C08G63/6852 ,  Y10S435/874

    摘要： A polyhydroxyalkanoate having a monomer unit composition represented by General Formula (1): AmB(1-m)  (1) wherein A is represented by General Formula (2), B is at least one selected from the group consisting of monomer units represented by General Formula (3) or (4), and m has a value of 0.01 or larger and smaller than 1: wherein n has a value of 0 to 10, k has a value of 3 or 5, and R is at least one group selected from the group consisting of groups represented by General Formulae (5) to (7): in Formula (5) R1 is a group selected from the group consisting of a hydrogen atom (H), halogen atoms, —CN, —NO2, —CF3, —C2F5. and —C3F7; and q is an integer selected from 1 to 8; in Formula (6) R2 is a group selected from the group consisting of a hydrogen atom (H), halogen atoms, —CN, —NO2, —CF3, —C2F5 and —C3F7; and r is an integer selected from 1 to 8; in Formula (7) R3 is a group selected from the group consisting of a hydrogen atom (H), halogen atoms, —CN, —NO2, —CF3, —C2F5 and —C3F7; and s is an integer selected from 1 to 8. The efficient production methods are also provided.

    摘要翻译： 具有由通式（1）表示的单体单元组成的聚羟基链烷酸酯：<？in-line-formula description =“In-line Formulas”end =“lead”？> A （1-m）（1）<？in-line-formula description =“In-line Formulas”end =“tail”？>其中A由通式（2）表示，B为至少一个 选自由通式（3）或（4）表示的单体单元组，并且m具有0.01以上且小于1的值：其中n具有0至10的值，k的值为3 或5，并且R是选自由通式（5）至（7）表示的基团中的至少一个基团：在式（5）中，R 1是选自氢原子（H） ，卤素原子，-CN，-NO 2，-CF 3，-C 2 H 5。 和-C 3 N 3 R 7; q为选自1至8的整数; 在式（6）中，R 2是选自氢原子（H），卤素原子，-CN，-NO 2，-CF 3， C 2 F 5和-C 3 F 7; 并且r是选自1至8的整数; 在式（7）中，R 3是选自氢原子（H），卤素原子，-CN，-NO 2，-CF 3 N， C 2 F 5和-C 3 F 7; s是选自1至8的整数。还提供了有效的制备方法。

    Polyhydroxyalkanoate, method for production thereof and microorganisms for use in the same
    3.
    发明授权
    Polyhydroxyalkanoate, method for production thereof and microorganisms for use in the same 失效
    聚羟基链烷酸酯，其制备方法和用于其的微生物

    公开(公告)号：US06649381B1

    公开(公告)日：2003-11-18

    申请号：US10411319

    申请日：2003-04-11

    申请人： Tsutomu Honma ,  Toyoko Kobayashi ,  Tetsuya Yano ,  Shin Kobayashi ,  Takeshi Imamura ,  Sakae Suda ,  Takashi Kenmoku

    发明人： Tsutomu Honma ,  Toyoko Kobayashi ,  Tetsuya Yano ,  Shin Kobayashi ,  Takeshi Imamura ,  Sakae Suda ,  Takashi Kenmoku

    IPC分类号： C12P762

    CPC分类号： C12P7/62 ,  C08G63/06 ,  C08G63/6822 ,  C08G63/6852 ,  Y10S435/874

    摘要： A polyhydroxyalkanoate having a monomer unit composition represented by General Formula (1): AmB(1−m)  (1)  wherein A is represented by General Formula (2), B is at least one selected from the group consisting of monomer units represented by General Formula (3) or (4), and m has a value of 0.01 or larger and smaller than 1:  wherein n has a value of 0 to 10, k has a value of 3 or 5, and R is at least one group selected from the group consisting of groups represented by General Formulae (5) to (7):  in Formula (5) R1 is a group selected from the group consisting of a hydrogen atom (H), halogen atoms, —CN, —NO2, —CF3, —C2F5 and —C3F7; and q is an integer selected from 1 to 8;  in Formula (6) R2 is a group selected from the group consisting of a hydrogen atom (H), halogen atoms, —CN, —NO2, —CF3, —C2F5 and —C3F7; and r is an integer selected from 1 to 8;  in Formula (7) R3 is a group selected from the group consisting of a hydrogen atom (H), halogen atoms, —CN, —NO2, —CF3, —C2F5 and —C3F7; and s is an integer selected from 1 to 8. The efficient production methods are also provided.

    摘要翻译： 具有由通式（1）表示的单体单元组成的聚羟基链烷酸酯：其中A由通式（2）表示，B为选自由通式（3）或（4）表示的单体单元中的至少一种， m的值为0.01以上且小于1：其中n为0〜10，k为3或5，R为选自由通式（1）表示的基团中的至少1种 5）至（7）：在式（5）中，R 1为选自氢原子（H），卤素原子，-CN，-NO 2，-CF 3，-C 2 F 5和-C 3 F 7的基团; q为选自1至8的整数;在式（6）中，R 2为选自氢原子（H），卤素原子，-CN，-NO 2，-CF 3，-C 2 F 5和-C 3 F 7 ; 并且r是选自1至8的整数;在式（7）中，R 3是选自氢原子（H），卤素原子，-CN，-NO 2，-CF 3，-C 2 F 5和-C 3 F 7的基团 ; s是选自1至8的整数。还提供了有效的制备方法。

    Polyhydroxyalkanoates and method of producing them by utilizing microorganisms
    4.
    发明授权
    Polyhydroxyalkanoates and method of producing them by utilizing microorganisms 失效
    聚羟基链烷酸酯及其利用微生物生产的方法

    公开(公告)号：US06521429B2

    公开(公告)日：2003-02-18

    申请号：US09745476

    申请日：2000-12-26

    申请人： Tsutomu Honma ,  Toyoko Kobayashi ,  Tetsuya Yano ,  Shin Kobayashi ,  Takeshi Imamura ,  Sakae Suda ,  Takashi Kenmoku

    发明人： Tsutomu Honma ,  Toyoko Kobayashi ,  Tetsuya Yano ,  Shin Kobayashi ,  Takeshi Imamura ,  Sakae Suda ,  Takashi Kenmoku

    IPC分类号： C12P762

    CPC分类号： C08G63/6852 ,  C08G63/06 ,  C08G63/6822 ,  C12P7/625 ,  Y10T428/2907

    摘要： A microbial polyhydroxyalkanoate which comprises one or more of monomer units represented by Formula (1), where R is at least one selected from the group represented by any one of Formulas (2), (3) and (4); in Formula (2), R1 is selected from the group consisting of hydrogen atom (H), halogen atom, —CN, —NO2, —CF3, —C2F5 and —C3F7, and q is an integer of 1 to 8; in Formula (3), R2 is selected from the group consisting of hydrogen atom (H), halogen atom, —CN, —NO2, —CF3, —C2F5 and —C3F7, and r is an integer of 1 to 8; in Formula (4), R3 is selected from the group consisting of hydrogen atom (H), halogen atom, —CN, —NO2, —CF3, —C2F5 and —C3F7, and s is an integer of 1 to 8. The production method is also disclosed.

    摘要翻译： 一种微生物聚羟基链烷酸酯，其包含一种或多种由式（1）表示的单体单元，其中R是选自式（2），（3）和（4）中任一项所示的基团中的至少一种;在式（2） ），R 1选自氢原子（H），卤素原子，-CN，-NO 2，-CF 3，-C 2 F 5和-C 3 F 7，q为1〜8的整数;在式（3）中， R2选自氢原子（H），卤素原子，-CN，-NO 2，-CF 3，-C 2 F 5和-C 3 F 7，r为1至8的整数;在式（4）中，R 3为 选自氢原子（H），卤素原子，-CN，-NO 2，-CF 3，-C 2 F 5和-C 3 F 7，s为1至8的整数。还公开了制备方法。

    Production method of polyester containing epoxy group in side chain and production method of crosslinked polymer
    7.
    发明授权
    Production method of polyester containing epoxy group in side chain and production method of crosslinked polymer 失效
    侧链中含有环氧基的聚酯的生产方法和交联聚合物的制备方法

    公开(公告)号：US06872788B2

    公开(公告)日：2005-03-29

    申请号：US10427919

    申请日：2003-05-02

    申请人： Takeshi Imamura ,  Etsuko Sugawa ,  Tetsuya Yano ,  Shin Kobayashi ,  Tsutomu Honma ,  Takashi Kenmoku

    发明人： Takeshi Imamura ,  Etsuko Sugawa ,  Tetsuya Yano ,  Shin Kobayashi ,  Tsutomu Honma ,  Takashi Kenmoku

    IPC分类号： C12N1/28 ,  C12P7/62 ,  C08F20/00

    CPC分类号： C12P7/625 ,  C12N1/28 ,  Y10S435/874

    摘要： A method is provided which biosynthesizes a PHA having an epoxy group on a side chain with improved physicochemical properties. Specifically, a method of producing a polyester containing an epoxy group in a side chain thereof using alkene as a raw material is provided which comprises the steps of bringing alkene into contact with a microorganism having an ability to uptake alkene and convert it to a polyester and allowing the microorganism to convert the alkene into a polyester containing an epoxy group in a side chain thereof. Further, a method of producing a crosslinked polymer is provided which comprises reacting the polyester obtained by the above mentioned method with a diamine compound.

    摘要翻译： 提供一种方法，其生物合成具有改善的物理化学性质的侧链上具有环氧基的PHA。 具体地说，提供了使用烯烃作为原料在其侧链中制备含有环氧基的聚酯的方法，其包括使烯与具有吸收烯烃的能力并将其转化为聚酯的微生物接触的步骤，以及 允许微生物将烯烃转化成其侧链中含有环氧基的聚酯。 此外，提供了一种制备交联聚合物的方法，其包括使通过上述方法获得的聚酯与二胺化合物反应。

    Polyhydroxyalkanoate synthase and gene encoding the same enzyme
    10.
    发明授权
    Polyhydroxyalkanoate synthase and gene encoding the same enzyme 失效
    聚羟基链烷酸酯合成酶和编码相同酶的基因

    公开(公告)号：US06808910B2

    公开(公告)日：2004-10-26

    申请号：US10266787

    申请日：2002-10-09

    申请人： Tetsuya Yano ,  Takeshi Imamura ,  Sakae Suda ,  Tsutomu Honma

    发明人： Tetsuya Yano ,  Takeshi Imamura ,  Sakae Suda ,  Tsutomu Honma

    IPC分类号： C12N902

    CPC分类号： C12P7/625 ,  C12N9/1025

    摘要： A novel polyhydroxyalkanoate (PHA) synthase derived from a microorganism capable of producing a PHA having a novel side-chain structure and a DNA encoding the amino acid sequence for the synthase are provided. Two PHA synthase proteins (SEQ ID Nos. 1 and 3) derived from Pseudomonas jessenii P161 (FERM BP-7376) and PHA synthase genes encoding these PHA synthases are provided, respectively (SEQ ID Nos. 2 and 4). A recombinant microorganism is endowed with a PHA producing ability.

    摘要翻译： 提供了源自能够产生具有新型侧链结构的PHA的微生物和编码合成酶的氨基酸序列的DNA的新型聚羟基链烷酸酯（PHA）合酶。 分别提供了两种衍生自琼脂糖假单胞菌P161（FERM BP-7376）的PHA合酶蛋白（SEQ ID No.1和3）和编码这些PHA合成酶的PHA合酶基因（SEQ ID No.2和4）。 重组微生物具有PHA产生能力。