公开(公告)号：US12105091B2

公开(公告)日：2024-10-01

申请号：US16761786

申请日：2018-11-06

申请人： VIB VZW ,  Universiteit Gent

发明人： An Hendrix ,  Olivier De Wever ,  Edward Geeurickx ,  Joke Vandesompele ,  Pieter Mestdagh ,  Sven Eyckerman

IPC分类号： G01N33/58 ,  C07K14/16 ,  C07K14/435 ,  C12Q1/6876 ,  G01N21/64 ,  G01N33/53

CPC分类号： G01N33/582 ,  C07K14/161 ,  C07K14/43595 ,  C12Q1/6876 ,  G01N21/6428 ,  G01N33/5308 ,  C07K2319/60 ,  C12Q2600/158 ,  G01N2021/6439 ,  G01N2496/30

摘要： This disclosure relates to the field of research and clinical usage of extracellular vesicles. More in particular, this disclosure relates to usages of recombinant extracellular vesicles comprising a) a self-assembling protein that directs its own release through vesicles as a luminal membrane-bound protein and b) a heterologous marker. Indeed, the disclosure provides recombinant extracellular vesicles that can be used as a biological reference material in methods to quantify extracellular vesicles in a sample, and/or, that can be used to calibrate a device for sample extracellular vesicles analysis, and/or, that can be used to evaluate the isolation process of extracellular vesicles. This disclosure thus relates to improving the accuracy of an extracellular vesicle-based diagnosis.

公开(公告)号：US20210181201A1

公开(公告)日：2021-06-17

申请号：US16761786

申请日：2018-11-06

申请人： VIB VZW ,  Universiteit Gent

发明人： An Hendrix ,  Olivier De Wever ,  Edward Geeurickx ,  Joke Vandesompele ,  Pieter Mestdagh ,  Sven Eyckerman

IPC分类号： G01N33/58 ,  C12Q1/6886 ,  G01N21/64

摘要： This disclosure relates to the field of research and clinical usage of extracellular vesicles. More in particular, this disclosure relates to usages of recombinant extracellular vesicles comprising a) a self-assembling protein that directs its own release through vesicles as a luminal membrane-bound protein and b) a heterologous marker. Indeed, the disclosure provides recombinant extracellular vesicles that can be used as a biological reference material in methods to quantify extracellular vesicles in a sample, and/or, that can be used to calibrate a device for sample extracellular vesicles analysis, and/or, that can be used to evaluate the isolation process of extracellular vesicles. This disclosure thus relates to improving the accuracy of an extracellular vesicle-based diagnosis.

公开(公告)号：US20220003775A1

公开(公告)日：2022-01-06

申请号：US17295383

申请日：2019-11-21

申请人： Universiteit Gent

发明人： An Hendrix ,  Olivier De Wever ,  Joeri Tulkens

IPC分类号： G01N33/68 ,  G01N33/92

摘要： The present disclosure relates to a method of analyzing a biofluid of a subject for the presence of bacterial extracellular vesicles (EV), the method comprising the steps of a) extracting bacterial EV from the biofluid, b) analyzing the bacterial EV-extracted molecular patterns for the presence of a disease marker, wherein the disease marker is a disease-specific molecular pattern of the subject; and the subject comprising patients diagnosed with HIV, IBD or cancer or the subject receiving a treatment.