公开(公告)号：US20200278345A1

公开(公告)日：2020-09-03

申请号：US16865780

申请日：2020-05-04

申请人： VIB VZW ,  Universiteit Gent

发明人： Jan Tavernier ,  Sven Eyckerman

IPC分类号： G01N33/543 ,  G01N33/68 ,  C07K14/005 ,  C12N7/00 ,  C12N9/06

摘要： This disclosure relates to a virus-like particle in which a small molecule-protein complex is entrapped, ensuring the formation of the small molecule-protein complex under physiological conditions, while protecting the small molecule-protein complex during purification and identification. The disclosure further relates to the use of such virus-like particle for the isolation and identification of small molecule-protein complexes.

公开(公告)号：US20190353650A1

公开(公告)日：2019-11-21

申请号：US16532154

申请日：2019-08-05

申请人： VIB VZW ,  Universiteit Gent

发明人： Jan Tavernier ,  Sven Eyckerman

IPC分类号： G01N33/543 ,  C07K14/005 ,  G01N33/68 ,  C12N7/00 ,  C12N9/06

摘要： This disclosure relates to a virus-like particle in which a small molecule-protein complex is entrapped, ensuring the formation of the small molecule-protein complex under physiological conditions, while protecting the small molecule-protein complex during purification and identification. The disclosure further relates to the use of such virus-like particle for the isolation and identification of small molecule-protein complexes.

公开(公告)号：US10444245B2

公开(公告)日：2019-10-15

申请号：US14402667

申请日：2013-05-24

申请人： VIB VZW ,  Universiteit Gent

发明人： Jan Tavernier ,  Sven Eyckerman

IPC分类号： G01N33/68 ,  C07K14/005 ,  C12N7/00 ,  C12N7/02 ,  C12N7/04

摘要： The disclosure relates to a virus-like particle in which a protein complex is entrapped, ensuring the formation of the protein complex under physiological conditions, while protecting the protein complex during purification and identification. The disclosure further relates to the use of such virus-like particle for the isolation and identification of protein complexes.

公开(公告)号：US12019076B2

公开(公告)日：2024-06-25

申请号：US17817908

申请日：2022-08-05

申请人： VIB VZW ,  Universiteit Gent

发明人： Nico Callewaert ,  Sven Eyckerman ,  Simon Devos

IPC分类号： G01N33/68 ,  C12N9/48 ,  C12N9/64

CPC分类号： G01N33/6812 ,  C12N9/485 ,  C12N9/641 ,  G01N33/68 ,  G01N33/6818 ,  G01N33/6824

摘要： The present invention relates to the field of biochemistry, more particularly to proteomics, more particularly to protein sequencing, even more particularly to single molecule peptide sequencing. The invention discloses methods for single molecule protein sequencing and/or amino acid identification using cleavage inducing agents which are not specific for one particular amino acid, cleave polypeptides step by step from the N-terminus onwards and provide information on the identity of the cleaved amino acids based on the kinetics.

公开(公告)号：US20220214354A1

公开(公告)日：2022-07-07

申请号：US17600437

申请日：2020-04-01

申请人： VIB VZW ,  UNIVERSITEIT GENT

发明人： Nico Callewaert ,  Simon Devos ,  Sven Eyckerman ,  Joel Vandekerckhove

IPC分类号： G01N33/68

摘要： The present application relates to the field of protein sequencing, more particularly to protein profiling using massively parallel sequencing with single-molecule sensitivity. Methods, assays and reagents are provided for sequencing individual protein or polypeptide molecules. Also provided are methods and assays for the parallel sequencing of proteins or polypeptides. To this end, particular labeled probes are used that are reactive with the N-terminal amino acid of the polypeptide molecules and can be detected while still associated with the polypeptide(s).

公开(公告)号：US10379115B2

公开(公告)日：2019-08-13

申请号：US15559748

申请日：2016-03-23

申请人： VIB VZW ,  Universiteit Gent

发明人： Jan Tavernier ,  Sven Eyckerman

IPC分类号： A61K39/12 ,  A61K39/21 ,  C07H21/04 ,  A61K39/00 ,  C07K14/005 ,  G01N33/543 ,  C12N7/00 ,  G01N33/68 ,  C12N9/06

摘要： This disclosure relates to a virus-like particle in which a small molecule-protein complex is entrapped, ensuring the formation of the small molecule-protein complex under physiological conditions, while protecting the small molecule-protein complex during purification and identification. The disclosure further relates to the use of such virus-like particle for the isolation and identification of small molecule-protein complexes.

公开(公告)号：US20180045719A1

公开(公告)日：2018-02-15

申请号：US15559748

申请日：2016-03-23

申请人： VIB VZW ,  Universiteit Gent

发明人： Jan Tavernier ,  Sven Eyckerman

IPC分类号： G01N33/543 ,  G01N33/68 ,  C12N7/00 ,  C07K14/005 ,  C12N9/06

CPC分类号： G01N33/5436 ,  C07K14/005 ,  C07K2319/43 ,  C07K2319/735 ,  C12N7/00 ,  C12N9/003 ,  C12N2740/16023 ,  C12N2740/16042 ,  C12N2740/16222 ,  G01N33/54313 ,  G01N33/54353 ,  G01N33/6803 ,  G01N33/6845 ,  G01N33/6848 ,  G01N2333/161 ,  G01N2333/90666

摘要： This disclosure relates to a virus-like particle in which a small molecule-protein complex is entrapped, ensuring the formation of the small molecule-protein complex under physiological conditions, while protecting the small molecule-protein complex during purification and identification. The disclosure further relates to the use of such virus-like particle for the isolation and identification of small molecule-protein complexes.

公开(公告)号：US11231416B2

公开(公告)日：2022-01-25

申请号：US16865780

申请日：2020-05-04

申请人： VIB VZW ,  Universiteit Gent

发明人： Jan Tavernier ,  Sven Eyckerman

IPC分类号： C07K14/705 ,  A61K38/17 ,  A61K38/00 ,  A61K9/00 ,  A61P35/00 ,  G01N33/543 ,  G01N33/68 ,  C07K14/005 ,  C12N7/00 ,  C12N9/06

摘要： This disclosure relates to a virus-like particle in which a small molecule-protein complex is entrapped, ensuring the formation of the small molecule-protein complex under physiological conditions, while protecting the small molecule-protein complex during purification and identification. The disclosure further relates to the use of such virus-like particle for the isolation and identification of small molecule-protein complexes.

公开(公告)号：US20200231956A1

公开(公告)日：2020-07-23

申请号：US16650230

申请日：2018-09-28

申请人： VIB VZW ,  UNIVERSITEIT GENT

发明人： Nico Callewaert ,  Sven Eyckerman ,  Simon Devos

IPC分类号： C12N9/48 ,  C12N9/64 ,  G01N33/68

摘要： The present invention relates to the field of biochemistry, more particularly to proteomics, more particularly to protein sequencing, even more particularly to single molecule peptide sequencing. The invention discloses means and methods for single molecule protein sequencing and/or amino acid identification using cleavage inducing agent. Said cleavage inducing agents which are not specific for one particular amino acid, cleave polypeptides step by step from the N-terminus onwards and provide information on the identity of the cleaved amino acids based on the kinetics of said reaction.

公开(公告)号：US20150153355A1

公开(公告)日：2015-06-04

申请号：US14402667

申请日：2013-05-24

申请人： VIB VZW ,  UNIVERSITEIT GENT

发明人： Jan Tavernier ,  Sven Eyckerman

IPC分类号： G01N33/68 ,  C12N7/00 ,  C07K14/005

CPC分类号： G01N33/6845 ,  C07K14/005 ,  C07K2319/20 ,  C07K2319/43 ,  C07K2319/735 ,  C12N7/00 ,  C12N7/02 ,  C12N7/045 ,  C12N2740/16023 ,  C12N2740/16222 ,  C12N2760/20222 ,  C12N2810/6081 ,  G01N33/6848 ,  G01N2333/15 ,  G01N2570/00

摘要： The disclosure relates to a virus-like particle in which a protein complex is entrapped, ensuring the formation of the protein complex under physiological conditions, while protecting the protein complex during purification and identification. The disclosure further relates to the use of such virus-like particle for the isolation and identification of protein complexes.

摘要翻译： 本公开涉及其中蛋白质复合物被捕获的病毒样颗粒，确保在生理条件下形成蛋白质复合物，同时在纯化和鉴定期间保护蛋白质复合物。 本公开还涉及这种病毒样颗粒用于分离和鉴定蛋白质复合物的用途。