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1.发明授权Synthesis and compositions of nucleic acids comprising 2′-terminator nucleotides 有权含有2'-终止子核苷酸的核酸的合成和组成公开(公告)号：US07928207B2
公开(公告)日：2011-04-19
申请号：US11583605
申请日：2006-10-18
CPC分类号： C12Q1/6827 , C07H21/00 , C07H21/04 , C12P19/34 , C12Q1/6848 , Y10S435/975 , C12Q2565/301 , C12Q2525/186 , C12Q2521/101
摘要： The invention provides methods of producing blocked oligonucleotides that include 2′-terminator nucleotides. These blocked oligonucleotides are used primers and probes in a variety of nucleic acid technologies. Related kits are also provided.
摘要翻译： 本发明提供了产生包含2'-终止子核苷酸的封闭寡核苷酸的方法。 在多种核酸技术中使用这些封闭的寡核苷酸引物和探针。 还提供相关工具包。
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公开(公告)号：US07745125B2
公开(公告)日：2010-06-29
申请号：US11583606
申请日：2006-10-18
IPC分类号： C12Q1/68
CPC分类号： C12P19/34 , C12Q1/6827 , C12Q1/6848 , C12Q1/6886 , C12Q2600/156 , C12Q2565/301 , C12Q2525/186 , C12Q2521/101
摘要： The present invention provides reaction mixtures that include blocked oligonucleotides comprising 2′-terminator nucleotides. The blocked oligonucleotides are rendered extendible when the 2′-terminator nucleotides are removed from the oligonucleotides, e.g., via pyrophosphorolysis. The reaction mixtures can be used in various nucleic acid polymerization and/or amplification assays, among many other applications. In addition to reaction mixtures, the invention also provides related methods and reaction mixtures.
摘要翻译： 本发明提供包含包含2'-终止子核苷酸的封闭寡核苷酸的反应混合物。 当从寡核苷酸中除去2'-终止子核苷酸时，例如通过焦磷酸分解，封闭的寡核苷酸可以延伸。 反应混合物可用于许多其它应用中的各种核酸聚合和/或扩增测定中。 除了反应混合物之外，本发明还提供相关方法和反应混合物。
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3.发明授权Alkyl amines improve detection of components of formaldehyde-fixed biological samples 有权烷基胺改善甲醛固定生物样品成分的检测公开(公告)号：US08652775B2
公开(公告)日：2014-02-18
申请号：US12079694
申请日：2008-03-28
IPC分类号： C12Q1/68
CPC分类号： G01N1/30 , C12Q1/6806 , C12Q2523/101
摘要： Alkyl amines act to release formaldehyde cross-linking that occurs in biological samples. Thus, contacting alkyl amines to formaldehyde fixed samples is a useful way to render biological components of the samples, including nucleic acids or proteins, more accessible to detection and characterization.
摘要翻译： 烷基胺用于释放在生物样品中发生的甲醛交联。 因此，使烷基胺与甲醛固定样品接触是使样品（包括核酸或蛋白质）的生物成分更易于检测和表征的有用方式。
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公开(公告)号：US07220847B2
公开(公告)日：2007-05-22
申请号：US10719257
申请日：2003-11-21
申请人： Veeraiah Bodepudi , Amar Gupta , Stephen G. Will
发明人： Veeraiah Bodepudi , Amar Gupta , Stephen G. Will
CPC分类号： C07H21/00 , C07H19/052 , C07H19/067 , C12Q1/34 , G01N33/533
摘要： The invention relates to detectable labels useful for detection of nucleotide sequences. Specifically, the invention relates to labeled-imidazole-PEG compounds, such as nucleosides, nucleotides, and nucleic acids incorporating such compounds, and methods utilizing such compounds. The invention further relates to kits comprising labeled imidazole-PEG compounds.
摘要翻译： 本发明涉及可用于检测核苷酸序列的可检测标记。 具体地，本发明涉及标记的咪唑-PEG化合物，例如核苷，核苷酸和掺入这些化合物的核酸，以及利用这些化合物的方法。 本发明还涉及包含标记的咪唑-PEG化合物的试剂盒。
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5.发明申请Alkyl amines improve detection of components of formaldehyde-fixed biological samples 有权烷基胺改善甲醛固定生物样品成分的检测公开(公告)号：US20090081661A1
公开(公告)日：2009-03-26
申请号：US12079694
申请日：2008-03-28
CPC分类号： G01N1/30 , C12Q1/6806 , C12Q2523/101
摘要： Alkyl amines act to release formaldehyde cross-linking that occurs in biological samples. Thus, contacting alkyl amines to formaldehyde fixed samples is a useful way to render biological components of the samples, including nucleic acids or proteins, more accessible to detection and characterization.
摘要翻译： 烷基胺用于释放在生物样品中发生的甲醛交联。 因此，使烷基胺与甲醛固定样品接触是使样品（包括核酸或蛋白质）的生物成分更易于检测和表征的有用方式。
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公开(公告)号：US07452674B1
公开(公告)日：2008-11-18
申请号：US11742097
申请日：2007-04-30
申请人： Veeraiah Bodepudi , Amar Gupta , Stephen G. Will
发明人： Veeraiah Bodepudi , Amar Gupta , Stephen G. Will
CPC分类号： C12Q1/6816 , C07H19/052
摘要： The invention relates to detectable labels useful for detection of nucleotide sequences. Specifically, the invention relates to labeled-imidazole-PEG compounds, such as nucleosides, nucleotides, and nucleic acids incorporating such compounds, and methods utilizing such compounds. The invention further relates to kits comprising labeled imidazole-PEG compounds.
摘要翻译： 本发明涉及可用于检测核苷酸序列的可检测标记。 具体地，本发明涉及标记的咪唑-PEG化合物，例如核苷，核苷酸和掺入这些化合物的核酸，以及利用这些化合物的方法。 本发明还涉及包含标记的咪唑-PEG化合物的试剂盒。
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公开(公告)号：US20080268441A1
公开(公告)日：2008-10-30
申请号：US11742097
申请日：2007-04-30
申请人： Veeraiah Bodepudi , Amar GUPTA , Stephen G. Will
发明人： Veeraiah Bodepudi , Amar GUPTA , Stephen G. Will
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6816 , C07H19/052
摘要： The invention relates to detectable labels useful for detection of nucleotide sequences. Specifically, the invention relates to labeled-imidazole-PEG compounds, such as nucleosides, nucleotides, and nucleic acids incorporating such compounds, and methods utilizing such compounds. The invention further relates to kits comprising labeled imidazole-PEG compounds.
摘要翻译： 本发明涉及可用于检测核苷酸序列的可检测标记。 具体地，本发明涉及标记的咪唑-PEG化合物，例如核苷，核苷酸和掺入这些化合物的核酸，以及利用这些化合物的方法。 本发明还涉及包含标记的咪唑-PEG化合物的试剂盒。
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公开(公告)号：US20110201002A1
公开(公告)日：2011-08-18
申请号：US13045427
申请日：2011-03-10
申请人： David Harrow Gelfand , Fred Lawrence Reichert , Veeraiah Bodepudi , Amar Gupta , Stephen Will , Thomas Myers
发明人： David Harrow Gelfand , Fred Lawrence Reichert , Veeraiah Bodepudi , Amar Gupta , Stephen Will , Thomas Myers
CPC分类号： C12Q1/6869 , Y10S435/975 , C12Q2535/101 , C12Q2525/101 , C12Q2525/186
摘要： The present invention provides methods of extending primer nucleic acids and sequencing target nucleic acids. The methods include the use of 2′-terminator nucleotides to effect chain termination. In addition to related reaction mixtures and kits, the invention also provides computers and computer readable media.
摘要翻译： 本发明提供扩增引物核酸和测序靶核酸的方法。 所述方法包括使用2'-终止子核苷酸来实现链终止。 除了相关的反应混合物和试剂盒之外，本发明还提供计算机和计算机可读介质。
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公开(公告)号：US07501504B2
公开(公告)日：2009-03-10
申请号：US11742123
申请日：2007-04-30
CPC分类号： C07H21/00 , C07H19/052 , C07H19/067 , C12Q1/34 , G01N33/533
摘要： The invention relates to detectable labels useful for detection of nucleotide sequences. Specifically, the invention relates to labeled-imidazole-PEG compounds, such as nucleosides, nucleotides, and nucleic acids incorporating such compounds, and methods utilizing such compounds. The invention further relates to kits comprising labeled imidazole-PEG compounds.
摘要翻译： 本发明涉及可用于检测核苷酸序列的可检测标记。 具体地，本发明涉及标记的咪唑-PEG化合物，例如核苷，核苷酸和掺入这些化合物的核酸，以及利用这些化合物的方法。 本发明还涉及包含标记的咪唑-PEG化合物的试剂盒。
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公开(公告)号：US20070208169A1
公开(公告)日：2007-09-06
申请号：US11742070
申请日：2007-04-30
申请人： Veeraiah Bodepudi , Amar Gupta , Stephen Will
发明人： Veeraiah Bodepudi , Amar Gupta , Stephen Will
IPC分类号： C07H19/052 , C07H19/04
CPC分类号： C07H21/00 , C07H19/052 , C07H19/067 , C12Q1/34 , G01N33/533
摘要： The invention relates to detectable labels useful for detection of nucleotide sequences. Specifically, the invention relates to labeled-imidazole-PEG compounds, such as nucleosides, nucleotides, and nucleic acids incorporating such compounds, and methods utilizing such compounds. The invention further relates to kits comprising labeled imidazole-PEG compounds.
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