摘要:
The present invention provides methods of extending primer nucleic acids and sequencing target nucleic acids. The methods include the use of 2′-terminator nucleotides to effect chain termination. In addition to related reaction mixtures and kits, the invention also provides computers and computer readable media.
摘要:
The present invention provides methods of extending nucleic acids and purifying target nucleic acids. The methods include the use of capping reagents to effect chain termination and provide a handle for purification via fluorous affinity methods.
摘要:
This invention provides compositions and methods for HCV typing, e.g., genotyping and/or subtyping. The compositions and methods of the invention can be used to assign an HCV isolate to one of at least five HCV types (for example, selected from types 1, 2, 3, 4, 5 or 6), or to one of at least five subtypes (for example, subtypes 1a/b/c, 2a/c, 2b, 3a, 4a, 5a or 6a). These methods integrate the hybridization data from a plurality of HCV typing probes in a multidimensional analysis to make an HCV type assignment for an HCV in an experimental sample. The invention also provides related compositions, including, for example, the HCV typing probes and HCV typing diagnostic kits.
摘要:
This invention provides compositions and methods for HCV typing, e.g., genotyping and/or subtyping. The compositions and methods of the invention can be used to assign an HCV isolate to one of at least five HCV genotypes (for example, selected from genotypes 1, 2, 3, 4, 5 or 6), or assign an HCV isolate to one of at least six subtypes (for example, selected from subtypes 1a/b/c, 2a/c, 2b, 3a, 4a, 5a or 6a), where the methods of the invention use only a single typing probe to make the HCV type assignment.
摘要翻译:本发明提供用于HCV分型的组合物和方法,例如基因分型和/或亚型。 本发明的组合物和方法可用于将HCV分离物分配至至少五种HCV基因型之一(例如,选自基因型1,2,3,4,5或6),或将HCV分离物分配至一个 至少六种亚型(例如,选自亚型1a / b / c,2a / c,2b,3a,4a,5a或6a),其中本发明的方法仅使用单一的分型探针来制备HCV型 分配。
摘要:
The present invention relates to methods and reagents for modifying the emission of light from labeled nucleic acids for the purpose of real time detection, analysis, and quantitation of nucleic acid sequences, e.g., using singly labeled probes. These methods and reagents exploit advantageous properties of thiazine dyes and diazine dyes. Furthermore, the use of these light emission modifiers in background reduction, nucleic acid duplex stabilization and other uses is also described. Related kits, reaction mixtures and integrated systems are described.
摘要:
A router and routing protocol redundancy are disclosed to reduce service outage or degradation for a network device and thus to increase service availability on a network due to software and hardware failures of the network device. A network device such as router includes a redundancy platform having an active controller system and a standby controller system. A routing protocol state change is received or generated by the active controller system. The received or generated routing protocol state change is replicated to the standby controller system. By replicating the routing protocol state change, the standby controller system can maintain the routing protocol sessions for the network device if a failure occurs in the active controller system. Furthermore, the routing protocol states are maintained in realtime to handle the dynamic changes created by routing protocols.
摘要:
Cryptographic apparatus, and a related method for its operation, for in-line encryption and decryption of data packets transmitted in a communication network. A full-duplex cryptographic processor is positioned between two in-line processing entities of a network architecture. For example, in a fiber distributed data interface (FDDI) network, the processor is positioned between a media access control (MAC) sublayer and a ring memory controller (RMC). Incoming information packets are analyzed to decide whether or not they contain encrypted data and, if they do, are subject to decryption before forwarding. Outbound information packets have their data portions encrypted if called for, and are usually forwarded toward the network communication medium. Cryptographic processing in both directions is performed in real time as each packet is streamed through the processor. The processing of outbound information packets includes using optional data paths for looping of the processed information back in a reverse direction, to permit the host system to perform local encryption or decryption for various purposes.
摘要:
The present invention provides methods of extending primer nucleic acids and sequencing target nucleic acids. The methods include the use of 2′-terminator nucleotides to effect chain termination. In addition to related reaction mixtures and kits, the invention also provides computers and computer readable media.
摘要:
The invention relates to detectable labels useful for detection of nucleotide sequences. Specifically, the invention relates to labeled-imidazole-PEG compounds, such as nucleosides, nucleotides, and nucleic acids incorporating such compounds, and methods utilizing such compounds. The invention further relates to kits comprising labeled imidazole-PEG compounds.
摘要:
The invention relates to detectable labels useful for detection of nucleotide sequences. Specifically, the invention relates to labeled-imidazole-PEG compounds, such as nucleosides, nucleotides, and nucleic acids incorporating such compounds, and methods utilizing such compounds. The invention further relates to kits comprising labeled imidazole-PEG compounds.