公开(公告)号：US20070010441A1

公开(公告)日：2007-01-11

申请号：US11356452

申请日：2006-02-17

申请人： Vernon Alvarez ,  Daniel O'Mahony ,  Imelda Lambkin ,  Catherine Patterson ,  Judith Singleton ,  Benjamin Belinka ,  John Carter ,  Gerard Cagney

发明人： Vernon Alvarez ,  Daniel O'Mahony ,  Imelda Lambkin ,  Catherine Patterson ,  Judith Singleton ,  Benjamin Belinka ,  John Carter ,  Gerard Cagney

IPC分类号： A61K38/17 ,  C07K14/47

CPC分类号： A61K38/28 ,  A61K47/58 ,  A61K47/593 ,  A61K47/60 ,  A61K47/62 ,  A61K47/6937

摘要： This invention relates to proteins (e.g., peptides) that are capable of facilitating transport of an active agent through a human or animal gastro-intestinal tissue, and derivatives (e.g., fragments) and analogs thereof, and nucleotide sequences coding for said proteins and derivatives. The proteins of the invention have use in facilitating transport of active agents from the lumenal side of the GIT into the systemic blood system, and/or in targeting active agents to the GIT. Thus, for example, by binding (covalently or noncovalently) a protein of the invention to an orally administered drug, the drug can be targeted to specific receptor sites or transport pathways which are known to operate in the human gastrointestinal tract, thus facilitating its absorption into the systemic system.

摘要翻译： 本发明涉及能够促进活性剂通过人或动物胃肠组织及其衍生物（例如片段）及其类似物以及编码所述蛋白质和衍生物的核苷酸序列的蛋白质（例如肽） 。 本发明的蛋白质用于促进活性剂从GIT的内腔侧输送到全身血液系统中，和/或将活性剂靶向GIT。 因此，例如，通过将本发明的蛋白质（共价或非共价）结合到口服给药的药物，该药物可以靶向已知在人胃肠道中操作的特异性受体位点或转运途径，从而有助于其吸收 进入系统系统。

公开(公告)号：US20100137151A1

公开(公告)日：2010-06-03

申请号：US12621652

申请日：2009-11-19

申请人： Andrew Emili ,  Gerard Cagney

发明人： Andrew Emili ,  Gerard Cagney

IPC分类号： C40B30/02

CPC分类号： G01N33/6818 ,  G16B15/00 ,  G16B30/00

摘要： This invention describes the use of peptide profiling to identify, characterize, and classify biological samples. In complex samples, many thousands of different peptides will be present at varying concentrations. The invention uses liquid chromatography and similar methods to separate peptides, which are then identified and quantified using mass spectrometry. By identification it is meant that the correct sequence of the peptide is established through comparisons with genome sequence databases, since the majority of peptides and proteins are unannotated and have no ascribed name or function. Quantification means an estimate of the absolute or relative abundance of the peptide species using mass spectrometry and related techniques including, but not limited to, pre- or post-experimental stable or unstable isotope incorporation, molecular mass tagging, is differential mass tagging, and amino acid analysis.

摘要翻译： 本发明描述了肽分析的用途来鉴定，表征和分类生物样品。 在复杂样品中，将以不同的浓度存在数千种不同的肽。 本发明使用液相色谱和类似方法分离肽，然后使用质谱鉴定和定量。 通过鉴定，这意味着通过与基因组序列数据库进行比较来建立肽的正确序列，因为大多数肽和蛋白质是未注释的，并且没有归属的名称或功能。 定量是指使用质谱法和相关技术估计肽种类的绝对或相对丰度，包括但不限于实验前或实验后稳定或不稳定的同位素掺入，分子量标记，差异质量标记和氨基 酸分析。

公开(公告)号：US06346613B1

公开(公告)日：2002-02-12

申请号：US09160496

申请日：1998-09-24

申请人： Daniel J. O'Mahony ,  Gerard Cagney

发明人： Daniel J. O'Mahony ,  Gerard Cagney

IPC分类号： C07H2104

CPC分类号： C12N15/1138 ,  A61K38/00 ,  A61K48/00 ,  C12N2310/315

摘要： A composition and method for enhancing paracellular transport across cell layers in an animal comprising an antisense oligonucleotide hybridizable with a region of the messenger RNA coding for the protein occludin which, when hybridized to the occludin mRNA, interferes with its translation such that occludin function is disrupted and paracellular permeability is increased across an epithelial cell layer or and endothelial cell layer in an animal.

摘要翻译： 一种用于增强动物中跨细胞层的细胞外转运的组合物和方法，包括可与编码蛋白质闭塞蛋白的信使RNA区域杂交的反义寡核苷酸，其与闭合蛋白mRNA杂交时会干扰其翻译，使得闭塞蛋白功能被破坏 并且跨动物的上皮细胞层或内皮细胞层增加细胞旁渗透性。

公开(公告)号：US20050048564A1

公开(公告)日：2005-03-03

申请号：US10479270

申请日：2002-05-30

申请人： Andrew Emili ,  Gerard Cagney

发明人： Andrew Emili ,  Gerard Cagney

IPC分类号： G01N33/68 ,  G06F19/16 ,  G06F19/22 ,  G01N33/53

CPC分类号： G01N33/6818 ,  G16B15/00 ,  G16B30/00

摘要： This invention describes the use of peptide profiling to identify, characterize, and classify biological samples. In complex samples, many thousands of different peptides will be present at varying concentrations. The invention uses liquid chromatography and similar methods to separate peptides, which are then identified and quantified using mass spectrometry. By identification it is meant that the correct sequence of the peptide is established through comparisons with genome sequence databases, since the majority of peptides and proteins are unannotated and have no ascribed name or function. Quantification means an estimate of the absolute or relative abundance of the peptide species using mass spectrometry and related techniques including, but not limited to, pre- or post-experimental stable or unstable isotope incorporation, molecular mass tagging, differential mass tagging, and amino acid analysis.

摘要翻译： 本发明描述了肽分析的用途来鉴定，表征和分类生物样品。 在复杂样品中，将以不同的浓度存在数千种不同的肽。 本发明使用液相色谱和类似方法分离肽，然后使用质谱鉴定和定量。 通过鉴定，这意味着通过与基因组序列数据库进行比较来建立肽的正确序列，因为大多数肽和蛋白质是未注释的，并且没有归属的名称或功能。 定量是指使用质谱法和相关技术估计肽种类的绝对或相对丰度，包括但不限于实验前或实验后稳定或不稳定的同位素掺入，分子量标记，差异质量标签和氨基酸 分析。