公开(公告)号：US07563778B2

公开(公告)日：2009-07-21

申请号：US10984919

申请日：2004-11-10

申请人： Karl-Hermann Schlingensiepen ,  Wolfgang Brysch

发明人： Karl-Hermann Schlingensiepen ,  Wolfgang Brysch

IPC分类号： A01N43/04 ,  C11Q1/68 ,  C12P19/34 ,  C07H21/02 ,  C07H21/04

CPC分类号： C12N15/1136 ,  A61K38/00 ,  C07H21/02 ,  C07H21/04 ,  C12N15/113 ,  C12N15/1135 ,  C12N15/1138 ,  C12N2310/11 ,  C12N2310/3125 ,  C12N2310/3145 ,  C12N2310/315 ,  C12N2310/3181 ,  C12N2310/351

摘要： A method for the preparation of an antisense oligonucleotide or derivative thereof comprising the steps of: selecting a target nucleic acid, if necessary elucidating its sequence; generating the antisense oligonucleotide with the proviso that: the oligonucleotide comprises at least 8 residues; the oligonucleotide comprises at maximum twelve elements, which are capable of forming three hydrogen bonds each to cytosine bases; the oligonucleotide does not contain four or more consecutive elements, capable of forming three hydrogen bonds each with four consecutive cytosine bases (CCCC) within the target molecule or alternatively four or more consecutive elements of GGGG; the oligonucleotide does also not contain 2 or more series of three consecutive elements, capable of forming three hydrogen bonds each with three consecutive cytosine bases (CCC) within the target molecule, or alternatively 2 or more series of three consecutive elements of GGG; and the ratio between residues forming two hydrogen bonds per residue (2H-bond-R) with the target molecule and those residues forming three hydrogen bonds per residue (3H-bond-R) with the target molecule, is ruled by the following specifications: 3H-bond-R/3H-bond-R+2H-bond-R≧0.29; and synthesizing the oligonucleotide thus generated in a per se known manner.

摘要翻译： 一种制备反义寡核苷酸或其衍生物的方法，包括以下步骤：如果需要，选择靶核酸阐明其序列; 产生反义寡核苷酸，条件是：寡核苷酸包含至少8个残基; 该寡核苷酸包含最多12个元件，其能够形成各自与胞嘧啶碱基的三个氢键; 寡核苷酸不含有四个或更多个连续的元件，能够在目标分子内形成三个氢键，每个具有四个连续的胞嘧啶碱基（CCCC），或者四个或更多个连续的GGGG元件; 寡核苷酸还不含有两个或更多个三个连续元件的系列，能够在目标分子内形成三个连接的胞嘧啶碱基（CCC）的三个氢键，或者另外两个或更多个三个连续的GGG元件系列; 并且与目标分子形成每个残基（2H-键-R）的两个氢键与每个残基形成三个氢键（3H-键-R）的残基与靶分子之间的比例由下列规格表示： 3H键-R / 3H键-R + 2H-键-R = 0.29; 并以本身已知的方式合成由此产生的寡核苷酸。

公开(公告)号：US06942979B1

公开(公告)日：2005-09-13

申请号：US09655272

申请日：2000-09-05

申请人： Eric Honore ,  Michel Fink ,  Michel Lazdunski ,  Florian Lesage ,  Fabrice Duprat

发明人： Eric Honore ,  Michel Fink ,  Michel Lazdunski ,  Florian Lesage ,  Fabrice Duprat

IPC分类号： G01N33/50 ,  A61K31/7088 ,  A61K38/00 ,  A61K39/395 ,  A61K48/00 ,  A61P5/00 ,  A61P9/00 ,  A61P21/00 ,  A61P25/00 ,  A61P25/28 ,  C07K14/705 ,  C07K16/28 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/00 ,  C12N5/10 ,  C12N15/09 ,  C12N15/12 ,  C12P21/02 ,  C12P21/08 ,  C12Q1/02 ,  G01N33/15 ,  G01N33/53 ,  C11Q1/68 ,  C07K21/04

CPC分类号： C12N5/00 ,  A61K38/00 ,  A61K48/00 ,  C07K14/705 ,  G01N33/53

摘要： A purified protein comprising a mechanosensitive potassium channel activated by at least one polyunsaturated fatty acid and riluzole and the use of such channels in drug screening.

摘要翻译： 一种纯化的蛋白质，其包含由至少一种多不饱和脂肪酸和利鲁唑激活的机械敏感钾通道，以及在药物筛选中使用这种通道。

公开(公告)号：US07138232B2

公开(公告)日：2006-11-21

申请号：US10154925

申请日：2002-05-28

申请人： Yuichi Hosoi

发明人： Yuichi Hosoi

IPC分类号： C11Q1/68 ,  C12M1/36 ,  C07H21/04 ,  G08F15/00

CPC分类号： G01N33/60 ,  C12Q1/6834 ,  C12Q2565/519 ,  C12Q2523/313

摘要： A method employing a porous material sheet which has at predetermined positions plural dots each being composed of a group of probe molecules and a stimulable phosphor sheet having a phosphor layer which contains a stimulable phosphor in an amount of 10 to 140 g/m2 is favorably utilized to detect by autoradiography radioactively labeled substances originating from living body or its analogues which are able to be fixed to the probe molecules by biochemically specific binding reaction.

摘要翻译： 使用多孔材料片的方法，该多孔材料片在预定位置由多个探针分子组成的多个点和具有10〜140g / m 2的含有可激发荧光体的荧光体层的可刺激荧光体片组成， 2有利地用于通过放射自显影来检测源自活体或其类似物的放射性标记物质，其能够通过生物化学特异性结合反应固定在探针分子上。

公开(公告)号：US07718364B2

公开(公告)日：2010-05-18

申请号：US10809965

申请日：2004-03-25

申请人： Dave S. B. Hoon ,  Bret Taback

发明人： Dave S. B. Hoon ,  Bret Taback

IPC分类号： C11Q1/68 ,  C07H21/02

CPC分类号： C12Q1/6886 ,  C12Q1/6806 ,  C12Q2600/112 ,  C12Q2600/118 ,  C12Q2600/154 ,  Y10T436/143333

摘要： A method is provided for assessing allelic losses and hypermethylation of genes in CpG tumor promotor region on specific chromosomal regions in cancer patients, including melanoma, neuroblastoma breast, colorectal, and prostate cancer patients. The method relies on the evidence that free DNA and hypermethylation of genes in CpG tumor promotor region may be identified in the bone marrow, serum, plasma, and tumor tissue samples of cancer patients. Methods of melanoma, neuroblastoma, colorectal cancer, breast cancer and prostate cancer detection, staging, and prognosis are also provided.

摘要翻译： 提供了一种用于评估癌症患者（包括黑素瘤，神经母细胞瘤乳腺癌，结肠直肠癌和前列腺癌患者）特定染色体区域CpG肿瘤启动子区域中等位基因损失和基因超甲基化的方法。 该方法依赖于可以在癌症患者的骨髓，血清，血浆和肿瘤组织样品中鉴定CpG肿瘤启动子区域中的游离DNA和基因的高甲基化的证据。 还提供了黑色素瘤，神经母细胞瘤，结肠直肠癌，乳腺癌和前列腺癌检测，分期和预后的方法。