公开(公告)号：US5948227A

公开(公告)日：1999-09-07

申请号：US992239

申请日：1997-12-17

申请人： Robert S. Dubrow

发明人： Robert S. Dubrow

IPC分类号： G01N27/447 ,  B01D57/02

CPC分类号： G01N27/44747 ,  G01N27/44752 ,  G01N27/44791

摘要： The present invention provides methods of electrophoretically separating macromolecular species, as well as compositions and systems useful in carrying out such methods. Specifically, the methods of the present invention comprise providing a substrate that has at least a first capillary channel disposed therein. The surface of the channel has a first surface charge associated therewith, and is filled with a water soluble surface adsorbing polymer solution that bears a net charge that is the same as the charge on the capillary surface.

摘要翻译： 本发明提供了电泳分离大分子物种的方法，以及用于实施这些方法的组合物和系统。 具体地，本发明的方法包括提供至少具有设置在其中的第一毛细通道的基底。 通道的表面具有与之相关的第一表面电荷，并填充有水溶性表面吸附聚合物溶液，其具有与毛细管表面上的电荷相同的净电荷。

公开(公告)号：US5585246A

公开(公告)日：1996-12-17

申请号：US328241

申请日：1994-10-24

申请人： Robert S. Dubrow ,  Bala S. Manian

发明人： Robert S. Dubrow ,  Bala S. Manian

IPC分类号： G01N33/49 ,  G01N15/14 ,  G01N33/533 ,  G01N33/569 ,  G01N33/53

CPC分类号： G01N15/1468 ,  G01N33/569 ,  G01N33/56972 ,  Y10S435/962 ,  Y10S435/968 ,  Y10S436/805 ,  Y10S436/825 ,  Y10S436/826 ,  Y10T436/101666 ,  Y10T436/107497 ,  Y10T436/108331

摘要： An assay and sample mixture for the enumeration of fluorescently stained target components of a whole blood sample by an imaging instrument. The sample preparation method ensures that the amount of target components per unit of volume of the whole blood sample is preserved by elimination of certain non-quantitative preparation steps while producing an even hematocrit layer within a scan capillary. Typical target components include white blood cells that express certain surface antigens, such as CD-4 and CD-8 proteins. To inhibit aggregation of the red blood cells, a reagent is added to an aliquot of whole blood sample. The aliquot of whole blood is mixed and with a preselected amount of a fluorescent dye and ligand complex which tags the target components. The sample and fluorescent complex are allowed to incubate a sufficient amount of time to bind enough of the fluorescent complex to the target components to provide a fluorescent signal from the target components which will be distinguishable from the fluorescent signal from the unbound fluorescent complex in the sample. The resulting mixture allows the imaging instrument to detect peak intensities of fluorescence from the target components, thereby allowing the target components to be volumetrically enumerated with an improved level of accuracy and efficiency.

公开(公告)号：US5547849A

公开(公告)日：1996-08-20

申请号：US236342

申请日：1994-05-02

申请人： Thomas M. Baer ,  Louis J. Dietz ,  Robert S. Dubrow ,  Paul G. Hayter ,  Michael Hodges ,  Bala S. Manian ,  Robert J. Shartle

发明人： Thomas M. Baer ,  Louis J. Dietz ,  Robert S. Dubrow ,  Paul G. Hayter ,  Michael Hodges ,  Bala S. Manian ,  Robert J. Shartle

IPC分类号： G01N33/49 ,  G01N15/14 ,  G01N21/78 ,  G01N33/543 ,  G01N33/554 ,  G06K9/00 ,  G01N33/536

CPC分类号： G06K9/00127 ,  G01N15/1468

摘要： The apparatus and method of the present invention disclose a scanning imaging cytometer wherein an unprocessed biological fluid sample is reacted with a fluorescently-labeled binding agent. The reacted sample undergoes minimal processing before it is placed into a capillary tube. The sample is optically scanned and fluorescence excitation is recorded from a plurality of columnar regions of the capillary tube, each columnar region generally defined by the spot size of the excitation beam and the depth dimension of the capillary tube. A spatial filter of a sufficient pinhole diameter is selected to allow simultaneous volumetric detection of all fluorescent targets in each columnar region.

摘要翻译： 本发明的装置和方法公开了一种扫描成像细胞仪，其中未处理的生物流体样品与荧光标记的结合剂反应。 反应的样品在放入毛细管之前经历最少的处理。 样品被光学扫描，并且从毛细管的多个柱状区域记录荧光激发，每个柱状区域通常由激发光束的光斑尺寸和毛细管的深度尺寸限定。 选择足够的针孔直径的空间滤波器以允许对每个柱状区域中的所有荧光目标的同时体积检测。

公开(公告)号：US5338427A

公开(公告)日：1994-08-16

申请号：US023088

申请日：1993-02-26

申请人： Robert J. Shartle ,  Robert S. Dubrow

发明人： Robert J. Shartle ,  Robert S. Dubrow

IPC分类号： G01N27/447 ,  C25B9/00

CPC分类号： G01N27/44704 ,  G01N27/44782

摘要： A disposable cartridge for a capillary electrophoresis instrument in which short capillary tube segments are used for simultaneous multiple lane separations. The cartridge contains all separation components of the instrument which come in contact with the sample and is capable of automatically loading a quantitative portion of a bulk sample into the capillary tube segments. Electrophoresis occurs without bulk flow through the capillaries, which are scanned in situ by the instrument.

摘要翻译： 用于毛细管电泳仪的一次性药筒，其中短毛细管段用于同时多道分离。 墨盒包含与样品接触的仪器的所有分离组分，并且能够将大量样品的定量部分自动加载到毛细管段中。 在没有大量流过毛细管的情况下进行电泳，通过仪器原位扫描。

公开(公告)号：US5083940A

公开(公告)日：1992-01-28

申请号：US267065

申请日：1988-11-04

申请人： Bruce Larson ,  Keith Dawes ,  Robert S. Dubrow

发明人： Bruce Larson ,  Keith Dawes ,  Robert S. Dubrow

IPC分类号： H01R4/70 ,  H01R13/52

CPC分类号： H01R4/70 ,  H01R13/5216

摘要： A gel and container means capable of protecting a binding post and drop wire from moisture and corrosion is provided which will effectively prevent the exposure of the drop wire and binding post cap and screw from moisture and corrosive atmospheres without the need for a pressure maintaining means other than the combination of the cap and gel. The pressure of the gel is maintained on the binding post through the extraction of plasticizers from the container means causing a shrinkage.

摘要翻译： 提供能够保护接合柱和掉线免受潮湿和腐蚀的凝胶和容器装置，其将有效地防止掉落线和接头柱盖和螺钉暴露于湿气和腐蚀性气氛中，而不需要压力保持装置 比帽和凝胶的组合。 通过从引起收缩的容器装置中提取增塑剂，将凝胶的压力保持在结合柱上。

公开(公告)号：US4950546A

公开(公告)日：1990-08-21

申请号：US233941

申请日：1988-08-18

申请人： Robert S. Dubrow ,  Catherine A. Dittmer ,  William D. Uken

发明人： Robert S. Dubrow ,  Catherine A. Dittmer ,  William D. Uken

IPC分类号： C08J7/04

CPC分类号： C08J7/047 ,  C08J2483/00 ,  Y10T428/31507 ,  Y10T428/31536 ,  Y10T428/31663

摘要： This invention provides for the radiation grafting of certain organopolysiloxanes to polymeric supports. The organopolysiloxane material is partially crosslinked mixture which contains from about 10% to about 90% by weight of uncrosslinked organopolysiloxane oil and has a cone penetration of about 100 to about 350 (10.sup.-1 mm) and an ultimate elongation of at least about 100%. The organopolysiloxane fluid may be placed on the polymeric support and crosslinked to produce an organopolysiloxane material having the above properties and being bonded to the polymeric support. The organopolysiloxane material also can be crosslinked to the above properties by conventional means then placed in contact with the polymeric support and irradiated to crosslink the material to the polymeric support without substantially changing the properties of the organopolysiloxane material.

摘要翻译： 本发明提供了某些有机聚硅氧烷对聚合物载体的辐射接枝。 有机聚硅氧烷材料是部分交联的混合物，其含有约10重量％至约90重量％的未交联的有机基聚硅氧烷油，并且具有约100至约350（10-1毫米）的锥形渗透和至少约100％的极限伸长率。 有机聚硅氧烷流体可以放置在聚合物载体上并交联以产生具有上述性质并结合到聚合物载体上的有机聚硅氧烷材料。 有机聚硅氧烷材料还可以通过常规方法与上述性能交联，然后与聚合物载体接触并照射以将材料交联聚合物载体，而基本上不改变有机聚硅氧烷材料的性质。

公开(公告)号：US4595635A

公开(公告)日：1986-06-17

申请号：US730692

申请日：1985-05-02

申请人： Robert S. Dubrow ,  Catherine A. Dittmer ,  William D. Uken

发明人： Robert S. Dubrow ,  Catherine A. Dittmer ,  William D. Uken

IPC分类号： C08J3/24 ,  B29C35/08 ,  B29C71/04 ,  C08J3/28 ,  C08J7/00 ,  C08J7/12 ,  B05D3/06

CPC分类号： B29C71/04 ,  B29C2035/0827 ,  B29K2083/00 ,  Y10T428/31663

摘要： This invention provides organopolysiloxane materials having cone penetration between about 100 and about 350 (10.sup.-1 mm) and ultimate elongation of at least 100% and having reduced surface tack. This invention provides three methods of obtaining said materials with reduced surface tack. The first method comprises applying to the surface of such material an organic peroxide or a photoinitiator and irradiating the surface with ultraviolet light to further crosslink the surface to reduce or eliminate the tack of the surface. The second method comprises mixing a photoinitiator, such as benzophenone, in an organopolysiloxane fluid, thermally crosslinking the organopolysiloxane fluid to produce the material with the above cone penetration and ultimate elongation then irradiating the surface of the material with ultraviolet light to reduce or eliminate the tack of the surface. The third method comprises mixing a photoinitiator, such as benzophenone, in an organopolysiloxane fluid, crosslinking the fluid by irradiating a layer of the fluid with ultraviolet light to produce the above cone penetration and ultimate elongation properties then further irradiating the crosslinked material to reduce or eliminate the surface tack; this method can be carried out by simultaneous irradiation with ultraviolet light from two sides of a layer of the organopolysiloxane wherein one surface is irradiated at a higher intensity than the other surface. The materials produced by this invention are useful as tapes and sealants.

摘要翻译： 本发明提供具有在约100至约350（10-1mm）之间的锥形穿透和至少100％的极限伸长率并具有降低的表面粘性的有机聚硅氧烷材料。 本发明提供了获得具有降低的表面粘性的所述材料的三种方法。 第一种方法包括将这种材料的表面施加到有机过氧化物或光引发剂上并用紫外光照射表面以进一步交联表面以减少或消除表面的粘性。 第二种方法包括将光引发剂如二苯甲酮混合在有机聚硅氧烷流体中，将有机聚硅氧烷流体热交联以产生具有上述锥体渗透和极限伸长的材料，然后用紫外光照射材料的表面以减少或消除粘性 的表面。 第三种方法包括将光引发剂如二苯甲酮混合在有机聚硅氧烷流体中，通过用紫外光照射一层流体来交联流体，以产生上述锥体渗透和极限伸长性能，然后进一步照射交联材料以减少或消除 表面粘性; 该方法可以通过同时照射来自有机聚硅氧烷层的两侧的紫外光进行，其中一个表面以比另一个表面更高的强度照射。 本发明生产的材料可用作带和密封剂。