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    Calibration method and apparatus for optical scanner
    2.
    发明授权
    Calibration method and apparatus for optical scanner 失效
    光学扫描仪的校准方法和装置

    公开(公告)号：US5689110A

    公开(公告)日：1997-11-18

    申请号：US670540

    申请日：1996-06-27

    申请人： Louis J. Dietz ,  Thomas M. Baer

    发明人： Louis J. Dietz ,  Thomas M. Baer

    IPC分类号： G01N21/64 ,  G01J3/28 ,  G01N15/10 ,  G01N21/27 ,  G01J3/443

    CPC分类号： G01N15/1012 ,  G01N21/274 ,  G01J2003/2866

    摘要： A calibration method and device for a fluorescence spectrometer which uses fluorescence from a homogeneous solid state standard as the source of calibration fluorescence and wherein the solid state standard may be built into the optical scanner and the calibration may be automatically performed as a routine step when using the optical scanner. A gold standard establishes fluorescent units, and the fluorescence spectrometer is calibrated by reference to calibration standards, such as calibration rubies, which are themselves rated against the gold standard and built into the fluorescence spectrometer to provide an unchanging reference to the gold standard and by which simultaneous calibration of two or more channels of a multi-channel fluorescence spectrometer may be accomplished, including automatically calibrating a multi-channel optical scanner when it is turned on to achieve an acceptable level of sensitivity in each channel and to adjust for any relative shift in sensitivity between the channels.

    摘要翻译： 用于荧光光谱仪的校准方法和装置，其使用来自均匀固态标准的荧光作为校准荧光源，并且其中固态标准可内置在光学扫描仪中，并且可以在使用时自动执行校准作为常规步骤 光学扫描仪。 黄金标准建立荧光单位，并且通过参考校准标准校准红宝石来校准荧光光谱仪，例如校准红宝石，其自身被评定为黄金标准，并被内置于荧光光谱仪中以提供对黄金标准的不变参考，并且由此 可以实现多通道荧光光谱仪的两个或更多个通道的同时校准，包括当其打开时自动校准多通道光学扫描仪以在每个通道中实现可接受的灵敏度水平并且调整任何相对偏移 通道之间的灵敏度。

    Laser microdissection apparatus and method
    3.
    发明授权
    Laser microdissection apparatus and method 有权
    激光显微解剖设备及方法

    公开(公告)号：US08715955B2

    公开(公告)日：2014-05-06

    申请号：US11222281

    申请日：2005-09-08

    申请人： Brian W. Donovan ,  Thomas M. Baer

    发明人： Brian W. Donovan ,  Thomas M. Baer

    IPC分类号： G01N1/28

    CPC分类号： G01N1/28 ,  G01N1/2813 ,  G01N1/286 ,  G01N1/30 ,  G01N1/44 ,  G01N2001/2826 ,  G01N2001/2833 ,  G01N2001/284 ,  G01N2001/2886 ,  Y10T156/10 ,  Y10T156/1054

    摘要： Systems and methods for automated laser microdissection are disclosed. In one variation, targeted biological material is manually or automatically selected and a transfer film is placed in juxtaposition to the location of an interior of a cut path. In another variation, a sample of biological material is mounted onto a polymer membrane which is then placed onto a substrate. Targeted biological material is manually or automatically selected and a transfer film is placed in juxtaposition with the targeted biological material on the side of the biological material. In yet another variation, a sample of biological material is mounted onto a polymer membrane which is then inverted onto a substrate. Targeted biological material is manually or automatically selected and a transfer film is placed in juxtaposition with the targeted biological material on the side of the polymer membrane. Then, an UV laser cuts along a cut path around the targeted portions of biological material in a closed cut path or a substantially closed cut path defining an interior and an exterior portion of the tissue sample. In a substantially closed cut path, bridges are left spanning the interior of the cut path and the exterior of the cut path. An IR laser activates at least a portion of the transfer film such that the transfer film in the vicinity of targeted portion adheres to the biological material interior to the cut path. The transfer film is then removed separating the targeted portions of biological material which are adhered to the transfer film from the remaining portion of the tissue sample.

    摘要翻译： 公开了用于自动激光显微切割的系统和方法。 在一个变型中，手动或自动选择目标生物材料，并且将转移膜并排放置在切割路径的内部的位置。 在另一个变体中，将生物材料样品安装在聚合物膜上，然后将其放置在基底上。 手动或自动选择靶向生物材料，并将转移膜与生物材料侧面上的靶向生物材料并置放置。 在另一个变体中，将生物材料样品安装在聚合物膜上，然后将其反转到基底上。 手动或自动选择目标生物材料，并将转移膜与聚合物膜侧面上的目标生物材料并置放置。 然后，UV激光沿着围绕生物材料的目标部分的切割路径沿封闭的切割路径或限定组织样本的内部和外部部分的基本封闭的切割路径切割。 在基本封闭的切割路径中，跨越切割路径的内部和切割路径的外部。 红外激光激活转印膜的至少一部分，使得目标部分附近的转印膜附着在生物材料内部至切割路径。 然后除去转移膜，从生物材料的目标部分分离出粘附到转移膜上的剩余部分的组织样品。

    Consumable for laser capture microdissection
    5.
    发明授权
    Consumable for laser capture microdissection 失效
    可用于激光捕获显微切割

    公开(公告)号：US07075640B2

    公开(公告)日：2006-07-11

    申请号：US08984979

    申请日：1997-12-04

    申请人： Thomas M. Baer ,  David F. Head ,  John Toeppen

    发明人： Thomas M. Baer ,  David F. Head ,  John Toeppen

    IPC分类号： G01N21/01

    CPC分类号： G01N1/2813 ,  G01N2001/028 ,  G01N2001/2833 ,  G01N2001/284

    摘要： Systems and methods for acquiring laser capture microdissection samples are disclosed. An integral portion of a biological reaction vessel includes a transfer film carrier having a substrate surface; and a laser capture microdissection transfer film coupled to the substrate surface of the transfer film carrier. The systems and methods facilitate quick and accurate laser capture microdissection while simultaneously minimizing contamination.

    摘要翻译： 公开了用于获取激光捕获显微解剖样品的系统和方法。 生物反应容器的整体部分包括具有基底表面的转移膜载体; 以及耦合到转印膜载体的基板表面的激光捕获显微切割转印膜。 系统和方法有助于快速准确的激光捕获显微切割，同时最大限度地减少污染。

    Laser capture microdissection analysis vessel
    10.
    发明授权
    Laser capture microdissection analysis vessel 失效
    激光捕获显微解剖分析容器

    公开(公告)号：US5859699A

    公开(公告)日：1999-01-12

    申请号：US797026

    申请日：1997-02-07

    申请人： Thomas M. Baer ,  David F. Head

    发明人： Thomas M. Baer ,  David F. Head

    IPC分类号： G01N33/48 ,  B01L3/00 ,  B01L3/14 ,  G01N1/00 ,  G01N1/02 ,  G01N1/28 ,  G02B21/34 ,  G01N1/10

    CPC分类号： B01L3/50853 ,  B01L3/50825 ,  G01N1/2813 ,  G02B21/34 ,  B01L2300/021 ,  B01L2300/047 ,  G01N2001/284

    摘要： A laser capture microdissection lift-off substrate comprising a transparent thermoplastic film is incorporated into substrate which may take the form of a cap or other structure associated with an analysis vessel or which may be used with a holder or other element of a LCM tissue sample transfer apparatus. The thermoplastic lift-off substrate film is held in a fixed position on the substrate which may be relative to a feature of a biological analysis vessel. The film may be affixed to a substrate which is a structure which goes into the vessel such that the sample is held in a fixed position relative to an observation port on the vessel.

    摘要翻译： 将包含透明热塑性薄膜的激光捕获显微切割剥离基底结合到基底中，基底可以采取与分析容器相关联的帽或其它结构的形式，或者可以与LCM组织样品转移的保持器或其他元件一起使用 仪器。 热塑性剥离基材膜保持在基板上的固定位置，其可以相对于生物分析容器的特征。 该膜可以固定到基底上，该基底是进入血管的结构，使得样品相对于血管上的观察孔保持在固定位置。