公开(公告)号：US10744463B2

公开(公告)日：2020-08-18

申请号：US16844330

申请日：2020-04-09

Applicant: Inscripta, Inc.

Inventor： Phillip Belgrader ,  Don Masquelier ,  Matthew Estes ,  Bruce Chabansky ,  Andrew Garst ,  Richard Fox ,  Jorge Bernate

IPC: B01D63/08 ,  B01D25/00

Abstract: The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methods—including high throughput methods—for screening cells that have been subjected to editing and identifying cells that have been properly edited.

公开(公告)号：US10724021B1

公开(公告)日：2020-07-28

申请号：US16868472

申请日：2020-05-06

Applicant: Inscripta, Inc.

Inventor： Juhan Kim ,  Benjamin Mijts ,  Aamir Mir ,  Andrew Garst ,  Kyle Seamon

IPC: C12N9/22 ,  C12N15/10 ,  C12N15/74 ,  C12N15/81 ,  C12N15/85 ,  C12N15/90

Abstract: The present disclosure provides novel RNA-guided enzymes for making rational and direct edits to the genome of live cells.

公开(公告)号：US20200181592A1

公开(公告)日：2020-06-11

申请号：US16798309

申请日：2020-02-22

Applicant: Inscripta, Inc.

Inventor： Benjamin Mijts ,  Juhan Kim ,  Aamir Mir ,  Kyle Seamon ,  Andrew Garst

IPC: C12N9/22

Abstract: The present disclosure provides engineered RNA-guided enzymes for editing live cells.

公开(公告)号：US20200071657A1

公开(公告)日：2020-03-05

申请号：US16686066

申请日：2019-11-15

Applicant: Inscripta, Inc.

Inventor： Charles Johnson ,  Miles Gander ,  Don Masquelier ,  Phillip Belgrader ,  Andrew Garst ,  Matthew Estes ,  Bruce Chabansky

IPC: C12M1/00 ,  C12N15/87 ,  C12M3/00 ,  C12M1/26

Abstract: The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methods—including high throughput methods—for screening cells that have been subjected to editing and identifying cells that have been properly edited.

公开(公告)号：US20200056139A1

公开(公告)日：2020-02-20

申请号：US16536049

申请日：2019-08-08

Applicant: Inscripta, Inc.

Inventor： Andrew Garst ,  Michael Graige ,  Richard Fox ,  Eileen Spindler ,  Amy Hiddessen ,  Phillip Belgrader ,  Don Masquelier ,  Bruce Chabansky

IPC: C12M1/42 ,  C12N15/11 ,  C12M1/26 ,  C12N15/90 ,  C12M3/00

Abstract: The present disclosure provides automated modules and instruments for improved detection of nuclease genome editing of live cells. The disclosure provides improved modules—including high throughput modules—for screening cells that have been subjected to editing and identifying and selecting cells that have been properly edited.

公开(公告)号：US10435714B2

公开(公告)日：2019-10-08

申请号：US15896433

申请日：2018-02-14

Applicant: Inscripta, Inc.

Inventor： Ryan T. Gill ,  Andrew Garst ,  Tanya Elizabeth Warnecke Lipscomb

IPC: C12N15/90 ,  C12N15/11 ,  C12N15/10 ,  C12N15/82 ,  C12N15/85 ,  C12N9/22 ,  C12N5/14 ,  C12N5/22 ,  C12N15/09 ,  C12N15/52 ,  C12N15/63 ,  C12N15/79 ,  C12N15/70 ,  C12N15/81 ,  C12N15/113

Abstract: Disclosed herein are nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Disclosed herein are engineered non-naturally occurring nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Targetable nuclease systems can be used to edit genetic targets, including recursive genetic engineering and trackable genetic engineering methods.

公开(公告)号：US10294473B2

公开(公告)日：2019-05-21

申请号：US15948793

申请日：2018-04-09

Applicant: The Regents of the University of Colorado, a body corporate ,  Inscripta, Inc.

Inventor： Ryan T. Gill ,  Andrew Garst ,  Tanya Elizabeth Warnecke Lipscomb ,  Marcelo Colika Bassalo ,  Ramsey Ibrahim Zeitoun

IPC: C12N15/10 ,  C12N15/11

Abstract: Provided herein are methods and composition for trackable genetic variant libraries. Further provided herein are methods and compositions for recursive engineering. Further provided herein are methods and compositions for multiplex engineering. Further provided herein are methods and compositions for enriching for editing and trackable engineered sequences and cells using nucleic acid-guided nucleases.

公开(公告)号：US10011849B1

公开(公告)日：2018-07-03

申请号：US15631989

申请日：2017-06-23

Applicant: Inscripta, Inc.

Inventor： Ryan T. Gill ,  Andrew Garst ,  Tanya Elizabeth Warnecke Lipscomb

IPC: C12N15/90 ,  C12N15/11 ,  C12N15/10 ,  C12N15/82 ,  C12N15/85 ,  C12N9/22 ,  C12N5/14 ,  C12N5/16 ,  C12N5/22 ,  C12N15/09 ,  C12N15/52 ,  C12N15/63 ,  C12N15/79 ,  C12N15/70 ,  C12N15/81

CPC classification number: C12N15/902 ,  C12N9/22 ,  C12N15/11 ,  C12N15/111 ,  C12N15/70 ,  C12N15/81 ,  C12N15/85 ,  C12N15/905 ,  C12N15/907 ,  C12N2310/20 ,  C12N2800/22

Abstract: Disclosed herein are nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Disclosed herein are engineered non-naturally occurring nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Targetable nuclease systems can be used to edit genetic targets, including recursive genetic engineering and trackable genetic engineering methods.