公开(公告)号：US08741611B2

公开(公告)日：2014-06-03

申请号：US12870523

申请日：2010-08-27

申请人： Stephen Brown ,  Sheryl Luttringer ,  Debbie Yaver ,  Alan Berry

发明人： Stephen Brown ,  Sheryl Luttringer ,  Debbie Yaver ,  Alan Berry

IPC分类号： C12P7/46 ,  C12N1/00

CPC分类号： C12P7/46 ,  C07K14/38 ,  C12N15/80

摘要： The present invention relates to methods of producing a C4 dicarboxylic acid, comprising: (a) cultivating a filamentous fungal host cell comprising a polynucleotide selected from the group consisting of a heterologous first polynucleotide encoding a C4 dicarboxylic acid transporter, a heterologous second polynucleotide encoding a malate dehydrogenase, and a heterologous third polynucleotide encoding a pyruvate carboxylase; wherein the filamentous fungal host cell is capable of secreting increased levels of the C4 dicarboxylic acid compared to the filamentous fungal host cell without the heterologous polynucleotide when cultivated under the same conditions; and (b) recovering the C4 dicarboxylic acid. The present invention also relates to methods for increasing C4 dicarboxylic acid production, filamentous fungal host cells and malate dehydrogenase variants.

公开(公告)号：US20120247190A1

公开(公告)日：2012-10-04

申请号：US13504520

申请日：2010-10-26

申请人： Stephen Brown ,  William Johns ,  Richard Phillips ,  Dale Rogers

发明人： Stephen Brown ,  William Johns ,  Richard Phillips ,  Dale Rogers

IPC分类号： G01N11/08

CPC分类号： G01N11/08

摘要： A rheometeris disclosed for determining flow characteristics of a fluid. The rheometer comprises a circuit in which the fluid is arranged to flow. The circuit comprises a duct and a plurality of flow elements, each comprising a flow environment arranged in fluid communication with the duct. The flow environment of each element is arranged to convey fluid from an up-stream position to a downstream position of the respective flow element. The rheometer further comprises pressure sensing means arranged to determine fluid pressure at the upstream position and the downstream position of each of the plurality of flow elements. At least two of the plurality of flow elements comprise different flow environments for the fluid such that the rheometer can determine the flow characteristics of the fluid at a particular instant in time.

摘要翻译： 公开了一种用于确定流体的流动特性的流变仪。 流变仪包括其中流体被布置成流动的电路。 电路包括管道和多个流动元件，每个流动元件包括布置成与管道流体连通的流动环境。 每个元件的流动环境布置成将流体从上游位置输送到相应流动元件的下游位置。 流变仪还包括压力感测装置，其布置成确定多个流动元件中的每一个的上游位置和下游位置处的流体压力。 多个流动元件中的至少两个流体元件包括用于流体的不同流动环境，使得流变仪可以在特定时刻确定流体的流动特性。

公开(公告)号：US20120149067A1

公开(公告)日：2012-06-14

申请号：US13401663

申请日：2012-02-21

申请人： Alan Sloma ,  Leslie Naggiar ,  Regine Behr ,  William Widner ,  Maria Tang ,  David Sternberg ,  Linda Sternberg ,  Stephen Brown

发明人： Alan Sloma ,  Leslie Naggiar ,  Regine Behr ,  William Widner ,  Maria Tang ,  David Sternberg ,  Linda Sternberg ,  Stephen Brown

IPC分类号： C12P19/28

CPC分类号： C12P19/26 ,  C12N9/1051 ,  C12N15/52

摘要： The present invention relates to methods for producing a hyaluronic acid, comprising: (a) cultivating a Bacillus host cell under conditions suitable for production of the hyaluronic acid, wherein the Bacillus host cell comprises a nucleic acid construct comprising a hyaluronan synthase encoding sequence operably linked to a promoter sequence foreign to the hyaluronan synthase encoding sequence; and (b) recovering the hyaluronic acid from the cultivation medium. The present invention also relates to an isolated nucleic acid sequence encoding a hyaluronan synthase operon comprising a hyaluronan synthase gene and a UDP-glucose 6-dehydrogenase gene, and optionally one or more genes selected from the group consisting of a UDP-glucose pyrophosphorylase gene, UDP-N-acetylglucosamine pyrophosphorylase gene, and glucose-6-phosphate isomerase gene. The present invention also relates to isolated nucleic acid sequences encoding a UDP-glucose 6-dehydrogenase, UDP-glucose pyrophosphorylase, and UDP-N-acetylglucosamine pyrophosphorylase

摘要翻译： 本发明涉及透明质酸的制备方法，其包括：（a）在适于产生透明质酸的条件下培养芽孢杆菌属宿主细胞，其中所述芽孢杆菌宿主细胞包含可操作地连接的透明质酸合酶编码序列的核酸构建体 涉及透明质酸合酶编码序列外源的启动子序列; 和（b）从培养基中回收透明质酸。 本发明还涉及编码包含透明质酸合酶基因和UDP-葡萄糖-6-脱氢酶基因的透明质酸合酶操纵子的分离的核酸序列，以及任选地一种或多种选自UDP-葡萄糖焦磷酸化酶基因， UDP-N-乙酰葡糖胺焦磷酸化酶基因和葡萄糖-6-磷酸异构酶基因。 本发明还涉及编码UDP-葡萄糖-6-脱氢酶，UDP-葡萄糖焦磷酸化酶和UDP-N-乙酰葡糖胺焦磷酸化酶的分离的核酸序列

公开(公告)号：USD639842S1

公开(公告)日：2011-06-14

申请号：US29376579

申请日：2010-10-08

申请人： Fouad Zeinoun ,  Stephen Brown ,  Jesse Moon ,  Andrea Casati

设计人： Fouad Zeinoun ,  Stephen Brown ,  Jesse Moon ,  Andrea Casati

公开(公告)号：USD639328S1

公开(公告)日：2011-06-07

申请号：US29376572

申请日：2010-10-08

申请人： Fouad Zeinoun ,  Stephen Brown ,  Jesse Moon ,  Andrea Casati

设计人： Fouad Zeinoun ,  Stephen Brown ,  Jesse Moon ,  Andrea Casati

公开(公告)号：US07887542B2

公开(公告)日：2011-02-15

申请号：US10931220

申请日：2004-08-31

申请人： Robert Metzger ,  Stephen Brown ,  Garrett A. Sheffer

发明人： Robert Metzger ,  Stephen Brown ,  Garrett A. Sheffer

IPC分类号： A61B17/15

CPC分类号： A61B17/155 ,  A61B17/02 ,  A61B17/14 ,  A61B17/142 ,  A61B17/157

摘要： Various method and apparatuses may be used to perform a procedure, such as a resection of a portion of the anatomy for preparation of the implants of a prosthetic. Various resecting member, including saw blades having selected geometries and shapes, can be used to assist in the resection of an anatomy to provide for implantation of a prosthetic. In addition, a cutting block assembly may be used to guide the resecting member. The cutting block assembly may be moveably mounted relative to the portion to be resected.

摘要翻译： 可以使用各种方法和装置来执行手术，例如切除解剖结构的一部分以准备假体的植入物。 可以使用包括具有选定几何形状和形状的锯片的各种切除构件来辅助切除解剖结构以提供假体植入。 此外，可以使用切割块组件来引导切割部件。 切割块组件可相对于要切除的部分可移动地安装。

公开(公告)号：US07797666B1

公开(公告)日：2010-09-14

申请号：US12244635

申请日：2008-10-02

申请人： Gordon Chiu ,  Deshanand Singh ,  Valavan Manohararajah ,  Stephen Brown

发明人： Gordon Chiu ,  Deshanand Singh ,  Valavan Manohararajah ,  Stephen Brown

IPC分类号： G06F17/50

CPC分类号： G06F17/5054

摘要： Systems and methods are provided for mapping logic functions from logic elements (“LEs”) into synchronous embedded memory blocks (“EMBs”) of programmable logic devices (“PLDs”). This technique increases the amount of logic that can fit into the PLD. Where area savings are significant, smaller PLDs may be selected to implement a particular circuit. One aspect of the invention relates to methods for identifying sequential cones of logic that may be mapped into synchronous EMBs. After the sequential logic cones are identified for mapping into a synchronous EMB, the logic cone may be selected, expanded, restructured, and retimed, as necessary, to implement the mapping. Another aspect of the invention relates to techniques for handling architectural restrictions of synchronous EMBs, such as the inability to implement the asynchronous behavior of synchronous logic.

摘要翻译： 提供了将逻辑元件（“LE”）的逻辑功能映射到可编程逻辑器件（“PLD”）的同步嵌入式存储器块（“EMB”）的系统和方法。 这种技术增加了可以适应PLD的逻辑量。 如果区域节省很大，则可以选择较小的PLD来实现特定的电路。 本发明的一个方面涉及用于识别可以映射到同步EMB中的逻辑顺序锥的方法。 在确定用于映射到同步EMB中的顺序逻辑锥之后，可以根据需要选择，扩展，重构和重新定时，以实现映射。 本发明的另一方面涉及用于处理同步EMB的架构限制的技术，诸如不能实现同步逻辑的异步行为。

公开(公告)号：US20100136630A1

公开(公告)日：2010-06-03

申请号：US12701926

申请日：2010-02-08

申请人： Stuart M. Stocks ,  Stephen Brown

发明人： Stuart M. Stocks ,  Stephen Brown

IPC分类号： C12P19/26

CPC分类号： C12P19/26

摘要： The present invention relates to methods for producing a hyaluronic acid with a desired average molecular weight in the range of 20,000-800,000 Dalton, the methods comprising the steps of: (a) cultivating a recombinant Bacillus host cell at a first temperature conducive to its growth, wherein the Bacillus host cell comprises a nucleic acid construct comprising a hyaluronan synthase encoding sequence operably linked to a promoter sequence foreign to the hyaluronan synthase encoding sequence; (b) then cultivating the recombinant Bacillus host cell of step (a) at a second temperature higher than the first temperature of step (a) under conditions suitable for production of the hyaluronic acid, whereby the Bacillus host cell produces hyaluronic acid with a desired average molecular weight in the range of 20,000-800,000 Dalton; and (c) recovering the hyaluronic acid.

摘要翻译： 本发明涉及制备具有20,000-800,000道尔顿范围内所需的平均分子量的透明质酸的方法，所述方法包括以下步骤：（a）在有利于其生长的第一温度下培养重组芽孢杆菌宿主细胞 其中所述芽孢杆菌宿主细胞包含核酸构建体，所述核酸构建体包含与透明质酸合酶编码序列异质的启动子序列可操作地连接的透明质酸合成酶编码序列; （b）然后在适于生产透明质酸的条件下，在高于步骤（a）的第一温度的第二温度下培养步骤（a）的重组芽孢杆菌宿主细胞，由此芽孢杆菌宿主细胞产生具有所需的透明质酸 平均分子量在20,000-800,000道尔顿范围内; 和（c）回收透明质酸。

公开(公告)号：US20090203002A1

公开(公告)日：2009-08-13

申请号：US12224766

申请日：2007-03-06

申请人： Stephen Brown

发明人： Stephen Brown

IPC分类号： C12Q1/68 ,  C12P19/34 ,  C40B40/08

CPC分类号： C12Q1/6883 ,  C12Q1/6881 ,  C12Q2600/154 ,  C12Q2600/156

摘要： The present invention provides a method of selectively amplifying fetal DNA sequences from a mixed, fetal-maternal source. This method utilizes differential methylation to allow for the selective amplification of trophoblast/fetal specific sequences from DNA mixtures that contain a high proportion of non-trophoblast/fetal DNA. The invention also provides methods of using the amplified fetal DNA sequences for aneuploidy detection.

摘要翻译： 本发明提供从混合胎儿母体来源中选择性扩增胎儿DNA序列的方法。 该方法利用差异甲基化来允许从包含高比例非滋养层/胎儿DNA的DNA混合物中选择性扩增滋养层/胎儿特异性序列。 本发明还提供了使用扩增的胎儿DNA序列进行非整倍体检测的方法。

公开(公告)号：US20080223390A1

公开(公告)日：2008-09-18

申请号：US10586751

申请日：2005-01-24

申请人： Stephen Brown

发明人： Stephen Brown

IPC分类号： A41G5/00

CPC分类号： A41G5/008 ,  A41G5/0046 ,  A41G5/0086

摘要： A hair extension method and application tool (10) bond a hair-piece, such as a hair braid (4), to an existing lock of hair (22) using an electro-magnetically curable adhesive which may be cured substantially athermally.

摘要翻译： 头发延伸方法和应用工具（10）使用可以基本上固化的电磁可固化粘合剂将诸如毛发编织物（4）的毛发粘合到现有的头发锁（22）上。