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    Methods for improving malic acid production in filamentous fungi
    1.
    发明授权
    Methods for improving malic acid production in filamentous fungi 失效

    公开(公告)号：US08741611B2

    公开(公告)日：2014-06-03

    申请号：US12870523

    申请日：2010-08-27

    申请人： Stephen Brown ,  Sheryl Luttringer ,  Debbie Yaver ,  Alan Berry

    发明人： Stephen Brown ,  Sheryl Luttringer ,  Debbie Yaver ,  Alan Berry

    IPC分类号： C12P7/46 ,  C12N1/00

    CPC分类号： C12P7/46 ,  C07K14/38 ,  C12N15/80

    摘要： The present invention relates to methods of producing a C4 dicarboxylic acid, comprising: (a) cultivating a filamentous fungal host cell comprising a polynucleotide selected from the group consisting of a heterologous first polynucleotide encoding a C4 dicarboxylic acid transporter, a heterologous second polynucleotide encoding a malate dehydrogenase, and a heterologous third polynucleotide encoding a pyruvate carboxylase; wherein the filamentous fungal host cell is capable of secreting increased levels of the C4 dicarboxylic acid compared to the filamentous fungal host cell without the heterologous polynucleotide when cultivated under the same conditions; and (b) recovering the C4 dicarboxylic acid. The present invention also relates to methods for increasing C4 dicarboxylic acid production, filamentous fungal host cells and malate dehydrogenase variants.

    Methods for C4-dicarboxylic acid production in filamentous fungi
    2.
    发明授权
    Methods for C4-dicarboxylic acid production in filamentous fungi 失效
    丝状真菌中C4-二羧酸生产方法

    公开(公告)号：US08497103B2

    公开(公告)日：2013-07-30

    申请号：US13165719

    申请日：2011-06-21

    申请人： Sheryl Luttringer ,  Debbie Yaver

    发明人： Sheryl Luttringer ,  Debbie Yaver

    IPC分类号： C12N1/15 ,  C12P7/46

    CPC分类号： C12P7/46 ,  C07K14/38

    摘要： The present invention relates to methods of producing C4-dicarboxylic acids, such as malic acid, comprising: (a) cultivating a host cell comprising a polynucleotide encoding a C4-dicarboxylic acid transporter; and (b) recovering the C4-dicarboxylic acid. The present invention also relates to methods for increasing C4-dicarboxylic acid production, as well as host cells comprising the polynucleotides.

    摘要翻译： 本发明涉及生产C4-二羧酸如苹果酸的方法，其包括：（a）培养包含编码C4-二羧酸转运蛋白的多核苷酸的宿主细胞; 和（b）回收C4-二羧酸。 本发明还涉及增加C4-二羧酸产生的方法以及包含多核苷酸的宿主细胞。

    Promoters for expressing genes in a fungal cell
    6.
    发明授权
    Promoters for expressing genes in a fungal cell 有权
    在真菌细胞中表达基因的启动子

    公开(公告)号：US09284588B2

    公开(公告)日：2016-03-15

    申请号：US13885145

    申请日：2011-12-15

    申请人： Sandra Merino ,  Debbie Yaver

    发明人： Sandra Merino ,  Debbie Yaver

    IPC分类号： C12P21/00 ,  C12N9/08 ,  C12N15/80

    CPC分类号： C12N15/80 ,  C12N9/0065 ,  C12P21/00

    摘要： The present invention relates to isolated promoters and constructs, vectors, and fungal host cells comprising such promoters operably linked to polynucleotides encoding polypeptides. The present invention also relates to methods for producing such polypeptides.

    摘要翻译： 本发明涉及分离的启动子和构建体，载体和包含与编码多肽的多核苷酸可操作地连接的此类启动子的真菌宿主细胞。 本发明还涉及生产这种多肽的方法。

    Methods For Producing Multiple Recombinant Polypeptides In A Filamentous Fungal Host Cell
    7.
    发明申请
    Methods For Producing Multiple Recombinant Polypeptides In A Filamentous Fungal Host Cell 有权
    在丝状真菌宿主细胞中产生多种重组多肽的方法

    公开(公告)号：US20140212977A1

    公开(公告)日：2014-07-31

    申请号：US14238691

    申请日：2012-08-23

    申请人： Debbie Yaver ,  Qiming Jin

    发明人： Debbie Yaver ,  Qiming Jin

    IPC分类号： C12N15/87 ,  C12N9/42 ,  C12N15/80

    CPC分类号： C12N9/2437 ,  C12N9/2402 ,  C12N15/80 ,  C12N15/87 ,  C12Y302/01091 ,  C12Y302/0115

    摘要： The present invention relates to methods for constructing a filamentous fungal strain for production of multiple recombinant polypeptides having biological activity. The present invention also relates to methods for producing multiple recombinant polypeptides having biological activity in a filamentous fungal strain. The present invention also relates to filamentous fungal strains expressing multiple recombinant polypeptides having biological activity.

    摘要翻译： 本发明涉及构建用于生产具有生物活性的多重重组多肽的丝状真菌菌株的方法。 本发明还涉及在丝状真菌菌株中生产具有生物活性的多重重组多肽的方法。 本发明还涉及表达具有生物活性的多种重组多肽的丝状真菌菌株。

    Promoter variants for expressing genes in a fungal cell
    8.
    发明授权
    Promoter variants for expressing genes in a fungal cell 有权
    用于在真菌细胞中表达基因的启动子变体

    公开(公告)号：US08604181B2

    公开(公告)日：2013-12-10

    申请号：US13424018

    申请日：2012-03-19

    申请人： Debbie Yaver ,  Peter Nham

    发明人： Debbie Yaver ,  Peter Nham

    IPC分类号： C07H21/04 ,  C12N15/63 ,  C12N15/00

    CPC分类号： C12N15/80 ,  C12N9/0044 ,  C12N9/20 ,  C12N9/58 ,  C12N9/93 ,  C12N15/113 ,  C12P1/02 ,  C12P21/00 ,  C12Y107/01003 ,  C12Y301/01003 ,  C12Y304/21004 ,  C12Y603/04002

    摘要： The present invention relates to methods for producing a biological substance, comprising: (a) cultivating a fungal host cell in a medium conducive for the production of the biological substance, wherein the fungal host cell comprises a first nucleic acid sequence encoding the biological substance operably linked to a second nucleic acid sequence comprising a promoter variant selected from the group consisting of SEQ ID NOs: 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, and 12; and a subsequence thereof; and hybrid and tandem promoters thereof; and (b) isolating the biological substance from the cultivation medium. The present invention also relates to the isolated promoter variants and to constructs, vectors, and fungal host cells comprising the promoter variants operably linked to nucleic acid sequences encoding biological substances.

    摘要翻译： 本发明涉及生物体的制造方法，其特征在于，包括：（a）在有助于生物生物的培养基中培养真菌宿主细胞，其中所述真菌宿主细胞可操作地包含编码生物物质的第一核酸序列 与包含选自SEQ ID NO：2,3,4,5,6,7,8,9,10,11和12的启动子变体的第二核酸序列连接; 及其后果; 及其杂交和串联启动子; 和（b）从培养基中分离生物物质。 本发明还涉及分离的启动子变体和构建体，载体和真菌宿主细胞，其包含可操作地连接于编码生物物质的核酸序列的启动子变体。