公开(公告)号：US11237130B2

公开(公告)日：2022-02-01

申请号：US15738361

申请日：2016-06-30

Applicant: Cytiva Sweden AB

Inventor： Lennart Bjorkesten ,  Nils Stafstrom

IPC: G01N27/447 ,  G01N30/86 ,  G01N21/64 ,  G06F17/15

Abstract: The present invention relates to a method for determining size of biomolecules separated in a medium by an electric field using marker molecules of known size, comprising —(101) detecting a plurality of bands and forming a detected marker sequence and a detected unknown sequence based on a separation criterion, —(102) determining band properties for each detected band, —(103) comparing the band properties of the detected bands of the detected marker sequence with known band properties for a plurality of marker molecules forming a known marker sequence and assigning a score to each comparison, said score being based on at least one of relative distance, relative intensity, expected distance and expected intensity between bands, —(104) selecting the comparison with the highest score and associating all or a subset of the detected bands of the detected marker sequence with said plurality of marker molecules of the known marker sequence in accordance with said comparison to determine size of the all or a subset of the detected marker sequence, and —(105) comparing the bands of the detected marker sequence with the bands of the detected unknown sequence to determine a size of biomolecules for each identified band of the detected unknown sequence based on the known sizes of the marker molecules. The invention also relates to software configured to perform the method and to a computer readable medium for storing said software.

公开(公告)号：US20220016853A1

公开(公告)日：2022-01-20

申请号：US17413702

申请日：2019-12-18

Applicant: Cytiva Sweden AB

Inventor： Hanish Lakhani ,  Preetam Hosamani ,  Nishu Garg ,  Bhaskaran Savarimuthu ,  Srihari Kannuri ,  Juzar Mazhar Ezzy ,  Ramu Balaguru

IPC: B29C65/00 ,  B29C65/82 ,  B29C65/04 ,  B29C65/14 ,  B29C65/16

Abstract: Disclosed is an automated method (54) which includes directing rays from a source (34) to a tube (38) disposed between relatively movable first and second sealing plates (20, 32), capturing an image (70) of at least a portion of the tube (38) by an image capturing device (26), and transferring the captured image (70) to a processing device (24). The method (54) also includes determining a plurality of 26 tube parameters by the processing device (24) based on the captured image (70), using an image processing technique and determining a plurality of sealing parameters from a database (44) by the processing device (24) based on the determined plurality of tube parameters. Additionally, the method (54) includes controlling the drive unit (22) and a heater (36) by the processing device (24) influenced by the determined plurality of sealing parameters, to respectively compress the tube (36)and perform heat sealing of the tube (38).

公开(公告)号：US20210395664A1

公开(公告)日：2021-12-23

申请号：US17277094

申请日：2019-09-20

Applicant: Cytiva Sweden AB

Inventor： Praveen Paul ,  Prashanth Hosabettu Mohan ,  Ajay Gore ,  Sahebagouda Alagur

IPC: C12M1/00 ,  B01D61/22 ,  B01D61/12 ,  C12M1/34

Abstract: A perfusion bioprocessing system (10) includes a bioreactor (12) and a recirculation flow path (14) provided with at least in one first feed flow control device (46) and at least one second feed control device (48). The perfusion bioprocessing system (10) further includes a first tangential flow filter (16) coupled to the bioreactor (12) via the recirculation flow path (14) and a second tangential flow filter (18) coupled to the bioreactor (12) via the recirculation flow path (14). The first tangential flow filter and the second tangential flow filter are coupled to a permeate flow path and a retentate flow path. Additionally, the perfusion bioprocessing system (10) includes a control unit (90) coupled to the at least one first feed flow control device (38) and the at least one second feed control device (40).

公开(公告)号：US20210387189A1

公开(公告)日：2021-12-16

申请号：US17281727

申请日：2019-10-16

Applicant: Cytiva Sweden AB

Inventor： Klaus Gebauer

IPC: B01L3/00

Abstract: The present invention relates to a bioprocessing fluid sensor arrangement (100) for sensing fluidic properties in a process fluid path with a sensor (S), configured for aseptically connecting the sensor with at least one conditioning fluid while separating said sensor from the process fluid to at least one conditioning fluid e.g. for calibration, cleaning, regenerating and/or storing the sensor, the arrangement comprising a process fluid path (PF) having a process fluid inlet (PI) and a process fluid outlet (PO); a sensor (S) arranged in the process fluid path (PF); a bypass fluid path (BF) in the process fluid path (PF), for bypassing the sensor (S); a conditioning or cleaning fluid path (CF) having an inlet (CI) and an outlet (CO) each aseptically and fluidically connected to the process fluid path (PF), one on each side of the sensor (S); and flow controls (FC) for controlling the flow of fluids, whereby fluids can be controlled to flow either in the process fluid path (PF) via the sensor (S), or in the bypass fluid path (BF) omitting the sensor from the fluid path (PF), or in the conditioning or cleaning fluid path (CF) including the sensor in said flow but omitting the remaining process fluid path (PF) and bypass fluid path (BF).

公开(公告)号：US20210322933A1

公开(公告)日：2021-10-21

申请号：US17363483

申请日：2021-06-30

Applicant: Cytiva Sweden AB

Inventor： Patrick Joseph McCloskey ,  Cathryn Ellen Olsen ,  Hongyi Zhou ,  Louisa Ruth Carr ,  Matthew Jeremiah Misner

IPC: B01D71/68 ,  B01D69/08 ,  A61M1/16 ,  B01D69/02 ,  B01D67/00 ,  B01D71/76

Abstract: A hollow-fiber membrane is presented. The hollow fiber membrane, includes a copolymer including sulfone structural units having a formula (I) and zwitterion-functionalized structural units having a formula (II). Method of making the hollow-fiber membrane is also presented.

公开(公告)号：US11137342B2

公开(公告)日：2021-10-05

申请号：US15565268

申请日：2016-04-08

Applicant: Cytiva Sweden AB

Inventor： Henrik Svanberg ,  Hanno Ehring ,  Erik Nils Ostlund

IPC: G01N21/05 ,  G02B6/38 ,  G02B6/36 ,  G02B6/42

Abstract: The present invention relates to an optical flow cell (1) for a measuring device, having an input light guide with a light exit surface, an output light guide with a light entrance surface, said input light guide and output light guide being integrated with a holder (30) to form optical flow cell (1), and wherein the holder (30) extends along a first axis (A) and has a through hole (31) for receiving a flow of a sample fluid, said through hole (31) being transversal to said first axis (A), and the input light guide and output light guide further are arranged in said holder (30) so that the light exit surface and the light entrance surface extend into said through hole (31) and are arranged to be in optical alignment with each other and at a first distance from each other. The invention also relates to a measuring device having at least one optical flow cell (1).

公开(公告)号：US11093583B2

公开(公告)日：2021-08-17

申请号：US15761570

申请日：2016-09-29

Applicant: CYTIVA SWEDEN AB

Inventor： Tobias Soderman ,  Viveca Lindahl

IPC: G06F17/18

Abstract: Methods and biosensor systems for improved evaluation of an interaction between an analyte in a fluid sample and a ligand immobilized on a sensor surface of a biosensor are provided. In one example, a method is provided which includes allowing a plurality of fluid samples to flow across a first sensor surface and a second sensor surface having a ligand immobilized thereon, where the fluid samples include a solvent (for example, an organic solvent with bulk effects such as DMSO) at known concentrations. The method further includes creating a data set for each fluid sample and forming a clean data set with outliers removed. Software for performing steps of methods disclosed and a computer readable medium for storing the software are also provided.

公开(公告)号：US20210215286A1

公开(公告)日：2021-07-15

申请号：US17056510

申请日：2019-05-29

Applicant: Cytiva Sweden AB

Inventor： Klaus Gebauer

IPC: F16L35/00 ,  A61M39/16

Abstract: The present invention relates to a connector for substantially aseptic connection, said connector comprising a connector member (1) having a connection end (13) and a coupling end (12), wherein the coupling end (12) comprises a coupling element (15) for engaging a similar coupling element (15′) on a similar connector (100) to form a first seal (B), and a cover (2) arranged to enclose the coupling end (12) for providing an aseptic seal that separates the coupling end (12) from a surrounding, the cover (2) comprising at least two cover portions (21A, 22A), each cover portion (21A, 22A) being configured to be successively-positioned on or adjacent the coupling end (12) of the connector member (1), wherein each cover portion (21A, 22A) comprises a seal member (21, 22) for engaging a similar seal member on a similar connector thereby to form a second seal (A) when so positioned on or adjacent the coupling end (12) of the connector member (1). The invention also relates to a method for connecting and disconnecting two such connectors.

公开(公告)号：US20210201032A1

公开(公告)日：2021-07-01

申请号：US17056484

申请日：2019-05-21

Applicant: Cytiva Sweden AB

Inventor： Petra Bangtsson ,  Johan Arthursson ,  Key Hyckenberg ,  Lotta Hedkvist

IPC: G06K9/00 ,  G06T11/00 ,  G06T7/00

Abstract: The present invention relates to a method of automated configuration in a bioprocess system (10) and to verify a process defined by a flow path representation. The method comprises the steps of: capturing one image of the bioprocess system (10) comprising tubing (50) for the fluid communication between units of the bioprocess system (10); analyzing the captured image to identify the tubing (50) connecting units of the bioprocess system (10); producing a processed representation from the captured image wherein at least a part of the tubing (50) is identified; and comparing the processed representation with the flow path representation to verify their functional concordance.

公开(公告)号：US20210162317A1

公开(公告)日：2021-06-03

申请号：US17268497

申请日：2019-08-20

Applicant: Cytiva Sweden AB

Inventor： Klaus Gebauer ,  Andreas Lundin ,  Fredrik Lundström

IPC: B01D15/18 ,  G01N30/20 ,  G01N30/38 ,  G01N30/46

Abstract: A connection device (1; 101) configured for connecting a separation system (3) with a separation device (5), wherein a fluid can be flowed from a separation system outlet connection (19), through a bypass fluid path (27) of the connection device to the separation system inlet connection (15) while a connected separation device (5) is by passed. For achieving this at least one of an inlet and an outlet valve (31, 33) provided in the connection device is provided in a closed state and at least one bypass valve (35, 35a, 35b) provided in the connection device is provided in an open state.