公开(公告)号：US08828357B2

公开(公告)日：2014-09-09

申请号：US13956245

申请日：2013-07-31

Applicant: Korea Basic Science Institute

Inventor： Chulhyun Lee ,  Sung Lan Jeon ,  Min Kyung Chae ,  Jee-Hyun Cho ,  Eun Ju Jang

IPC: A61B5/055 ,  A61K9/51 ,  A61K49/18 ,  C09C1/24 ,  C01G49/04 ,  B82Y30/00 ,  B82Y5/00 ,  B82Y15/00 ,  C01G49/08

CPC classification number: A61B5/055 ,  A61K9/51 ,  A61K9/5115 ,  A61K49/1836 ,  A61K49/186 ,  A61K49/1863 ,  B82Y5/00 ,  B82Y15/00 ,  B82Y30/00 ,  C01G49/04 ,  C01G49/08 ,  C01P2002/72 ,  C01P2004/04 ,  C01P2004/34 ,  C01P2004/64 ,  C09C1/24 ,  Y10S977/773 ,  Y10S977/788 ,  Y10S977/81 ,  Y10S977/906 ,  Y10S977/93

Abstract: Iron oxide nano contrast agents for Magnetic Resonance Imaging which have superior T2 contrast effect, and also can be used as a storage or a carrier for drugs and so on, are disclosed. The iron oxide nano contrast agents can be prepared by the steps of: coating surfaces of hydrophobic FeO nanoparticles with a coating material selected from the group consisting of polyethylene glycol-phospholipid conjugate, dextran, chitosan, dimercaptosuccinic acid and mixtures thereof in an organic solvent to form hydrophilic FeO nanoparticles having hydrophilic surfaces and dispersibility in water; dispersing the hydrophilic FeO nanoparticles in water to oxidize FeO; and exposing the oxidized hydrophilic FeO nanoparticles to an acidic buffer to dissolve and remove interior unoxidized FeO portions, and thereby to form Fe3O4 nanoparticles having an interior space.

Abstract translation: 公开了具有优异T2对比效果的磁共振成像用氧化铁纳米造影剂，也可用作药物的储存或载体等。 氧化铁纳米对比剂可以通过以下步骤制备：用有机溶剂中的选自聚乙二醇 - 磷脂缀合物，葡聚糖，壳聚糖，二巯基琥珀酸及其混合物的涂层材料涂覆疏水性FeO纳米颗粒的表面， 形成具有亲水表面和在水中分散性的亲水性FeO纳米颗粒; 将亲水性FeO纳米颗粒分散在水中以氧化FeO; 并将氧化的亲水性FeO纳米颗粒暴露于酸性缓冲液以溶解和除去内部未氧化的FeO部分，从而形成具有内部空间的Fe 3 O 4纳米颗粒。

公开(公告)号：US20130130273A1

公开(公告)日：2013-05-23

申请号：US13733687

申请日：2013-01-03

Applicant: Korea Basic Science Institute

Inventor： Zee Won Lee ,  Soo Hyun Kim

IPC: C12Q1/02 ,  G01N33/53

CPC classification number: C12Q1/025 ,  C07K2319/01 ,  C07K2319/60 ,  C12N15/1055 ,  G01N33/5035 ,  G01N33/542 ,  G01N33/557

Abstract: Disclosed is a novel method for detecting interactions of biomolecules. More particularly, the disclosed method includes (a) preparing a cell comprising (i) a first construct comprising a bait, a first labeling material and a translocation module; and (ii) a second construct comprising a prey and a second labeling material; (b) detecting the distribution of the first construct and the second construct in the cell. the present invention provides a method capable of detecting bindings and interactions occurring in a living cell in real time, and a method for screening a material that alters the binding and the interaction. The method of the present invention overcomes the disadvantages including inaccuracy and complexity of existing biomaterial interaction detection techniques. By labeling both constructs to promote accuracy, the present invention provides a novel real-time, antibody-free analysis.

Abstract translation: 公开了一种检测生物分子相互作用的新方法。 更具体地，所公开的方法包括（a）制备细胞，其包括（i）包含诱饵，第一标记材料和易位模块的第一构建体; 和（ii）包含猎物和第二标签材料的第二构建体; （b）检测细胞中第一构建体和第二构建体的分布。 本发明提供能够实时检测活细胞中发生的结合和相互作用的方法，以及筛选改变结合和相互作用的材料的方法。 本发明的方法克服了现有生物材料相互作用检测技术的不准确性和复杂性的缺点。 通过标记两种构建体以促进准确性，本发明提供了一种新颖的实时抗体免疫分析。