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16 条记录 (耗时 0.018 秒)

    METHODS FOR IMPROVEMENT OF BIRTH RATES IN CANIDAE ON SOMATIC CELL NUCLEAR TRANSFER
    11.
    发明申请
    METHODS FOR IMPROVEMENT OF BIRTH RATES IN CANIDAE ON SOMATIC CELL NUCLEAR TRANSFER 审中-公开
    改善角膜细胞核转移中生殖系统发生率的方法

    公开(公告)号：US20100293626A1

    公开(公告)日：2010-11-18

    申请号：US12517567

    申请日：2008-11-19

    申请人： Byeong Chun Lee ,  Min Kyu Kim ,  Goo Jang ,  Hyun Ju Oh ,  So Gun Hong ,  Jung Eun Park ,  Jung Taek Kang

    发明人： Byeong Chun Lee ,  Min Kyu Kim ,  Goo Jang ,  Hyun Ju Oh ,  So Gun Hong ,  Jung Eun Park ,  Jung Taek Kang

    IPC分类号： C12N15/06

    CPC分类号： C12N5/16 ,  A01K67/0273 ,  A01K2227/10 ,  C12N15/877 ,  C12N2501/405 ,  C12N2517/10

    摘要： The present invention relates to a method for increasing the efficiency of offspring production in producing animals belonging to the family Canidae (canines) by somatic cell nuclear transfer. More specifically, relates to a method for increasing the efficiency of production of cloned canines by a method for cloning canines comprising enucleating the oocyte of a canine to prepare an enucleated oocyte, fusing a nuclear donor cell with the enucleated oocyte to prepare a nuclear transfer embryo and transferring the nuclear transfer embryo into the oviduct of a surrogate mother, wherein the nuclear donor cell is cultured in a medium containing a specific cell cycle synchronization-inducing substance such as roscovitine in the preparation thereof. The method enables to clone canines with high efficiency, and thus can contribute to the development of studies in the fields of veterinary medicine, anthropology and medical science such as the propagation of superior canines, the conservation of rare or nearly extinct canines, xenotransplantation and disease animal models.

    摘要翻译： 本发明涉及通过体细胞核转移提高属于犬科（犬科）的动物的后代生产效率的方法。 更具体地，涉及通过克隆犬的方法来提高克隆犬的生产效率的方法，包括将犬的卵母细胞去核以制备去核卵母细胞，将核供体细胞与去核卵母细胞融合以制备核转移胚 并将核转移胚胎转移到替代母体的输卵管中，其中在其制备中，在含有特定细胞周期同步诱导物质如roscovitine的培养基中培养核供体细胞。 该方法能够高效地克隆犬科动物，从而有助于兽医，人类学和医学领域的研究发展，如高等犬传播，保护罕见或几乎灭绝的犬，异种移植和疾病 动物模型。

    Cloned Canines and Method for Producing Thereof
    15.
    发明申请
    Cloned Canines and Method for Producing Thereof 审中-公开
    克隆犬及其生产方法

    公开(公告)号：US20100287635A1

    公开(公告)日：2010-11-11

    申请号：US11989502

    申请日：2006-07-26

    申请人： Woo Suk Hwang ,  Byeong Chun Lee ,  Sung Keun Kang ,  Min Kyu Kim ,  Goo Jang ,  Hyun Ju Oh ,  Hye Jin Kim ,  Joung Ju Kim ,  Shamim Hossein Mohammad ,  Heru Fibrianto Yuda

    发明人： Woo Suk Hwang ,  Byeong Chun Lee ,  Sung Keun Kang ,  Min Kyu Kim ,  Goo Jang ,  Hyun Ju Oh ,  Hye Jin Kim ,  Joung Ju Kim ,  Shamim Hossein Mohammad ,  Heru Fibrianto Yuda

    IPC分类号： C12N15/89 ,  A01K67/027 ,  C12N15/63

    CPC分类号： C12N15/877

    摘要： Disclosed herein are a cloned canine and a production method thereof. The method comprises the steps of enucleating the oocyte of a canine to prepare an enucleated recipient oocyte, conducting nuclear transfer into the enucleated oocyte using a canine somatic cell as a nuclear donor cell under optimized conditions so as to prepare a nuclear transfer embryo, and transferring the nuclear transfer embryo into the oviduct of a surrogate mother. The present invention provides a method for producing cloned canines and thus, can contribute to the development of studies in veterinary medicine, anthropology and medical science such as the propagation of superior canines, the conservation of rare or nearly extinct canines, xenotransplantation and disease animal models.

    摘要翻译： 本文公开了克隆的犬及其制备方法。 该方法包括以下步骤：摘除犬的卵母细胞以制备去核的受体卵母细胞，在优化的条件下使用犬体细胞作为核供体细胞进行核转移到去核卵母细胞中，以制备核转移胚，并转移 核转移胚胎进入代孕母亲的输卵管。 本发明提供了一种克隆犬的生产方法，从而有助于发展兽医学，人类学和医学科学的研究，如高级犬的繁殖，珍稀或几乎灭绝的犬的保护，异种移植和疾病动物模型 。