公开(公告)号：US5741639A

公开(公告)日：1998-04-21

申请号：US396310

申请日：1995-02-28

申请人： Kees Ensing ,  Peter Oroszlan ,  Aran Paulus ,  Carlo S. Effenhauser

发明人： Kees Ensing ,  Peter Oroszlan ,  Aran Paulus ,  Carlo S. Effenhauser

IPC分类号： G01N33/543 ,  G01N27/447 ,  G01N33/561 ,  C12Q1/68

CPC分类号： G01N33/561 ,  Y10S435/96 ,  Y10S435/961 ,  Y10S436/81

摘要： A device for combined bioaffinity assay and electrophoretic separation is provided, which comprises a capillary system having two stages, a first stage in which bioaffinity interactions of analyte molecules and molecular recognition elements are performed, and a second stage, in which electrophoretic separation of the analyte molecules and subsequent detection of the separated species is accomplished. Within the first capillary stage the molecular recognition elements are attached and immobilized to the inside capillary wall, for example, by adsorption or by covalent binding to the capillary material. The method for combined bioaffinity assay and electrophoretic separation comprises flowing an analyte through a capillary system having two stages. In a first capillary stage the analyte molecules are captured by respective molecular recognition elements present in that stage. More particularly the analyte molecules are captured by molecular recognition elements which are attached and immobilized to the inside wall of that capillary stage, for example, by adsorption or by covalent binding to the capillary material. After a predetermined time the analyte-molecules are dissociated from the molecular recognition elements. Subsequently the analyte-molecules are separated in a second stage of the capillary system by capillary electrophoresis and finally the separated species are detected at the terminal part of the capillary system.

摘要翻译： 提供了用于组合生物亲和测定和电泳分离的装置，其包括具有两个阶段的毛细管系统，其中进行分析物分子和分子识别元件的生物亲和力相互作用的第一阶段，以及分析物的电泳分离的第二阶段 分子和随后的分离物质的检测是完成的。 在第一毛细血管阶段内，例如通过吸附或通过与毛细管材料的共价结合将分子识别元件附着并固定到内部毛细管壁。 用于组合生物亲和测定和电泳分离的方法包括使分析物流过具有两个阶段的毛细管系统。 在第一毛细血管阶段，分析物分子被该阶段中存在的相应的分子识别元件捕获。 更具体地，分析物分子被分子识别元件捕获，分子识别元件例如通过吸附或通过与毛细管材料的共价结合而附着和固定到该毛细管阶段的内壁。 在预定时间后，分析物 - 分子与分子识别元件分离。 随后，通过毛细管电泳在毛细管系统的第二阶段分离分析物分子，最后在毛细管系统的末端部分检测分离的物质。

公开(公告)号：US5599432A

公开(公告)日：1997-02-04

申请号：US335610

申请日：1994-11-08

申请人： Andreas Manz ,  Carlo S. Effenhauser

发明人： Andreas Manz ,  Carlo S. Effenhauser

IPC分类号： B01D57/02 ,  G01N27/447 ,  G01N37/00 ,  G01N27/26

CPC分类号： G01N27/44773

摘要： A device for the electrophoretic separation of complex fluid substance mixtures comprises a channel system (21, 2, 22) for a carrier medium (C), an injection device (3) for the injection into the carrier medium (C) of a substance mixture (S) to be separated, and a separating path (2) for the separation of the substance mixture (S) in an electric field applied along the separating path (2). Downstream of the injection device (3) for the substance mixture (S) to be separated and at a distance therefrom there is provided a second separating path (4) for the further separation of the substance mixture (S) in an electric field applied along the second separating path (4). The second separating path (4) is inclined at an angle (.alpha.) with respect to the first separating path (2). The region of intersection of the first (2) and second (4) separating paths forms a second injection device (5) for injecting the partially separated substance mixture (S) into a second carrier medium (E). The fluid substance mixture (S) is injected into the carrier medium (C) in the first injection device (3) and is then separated in the electric field that is applied along the first separating path (2). The partially separated substance mixture (S) is then injected into the second carrier medium (E) downstream of the first separating path (2) and further separated into its components in the electric field applied along the second separating path (4).

摘要翻译： 用于电泳分离复杂流体物质混合物的装置包括用于载体介质（C）的通道系统（21,2,22），用于注入物质混合物的载体介质（C）的注射装置（3） （S）和用于沿着分离路径（2）施加的电场中分离物质混合物（S）的分离路径（2）。 在用于待分离的物质混合物（S）的注射装置（3）的下游距离处设置有用于在沿着电场施加的电场中进一步分离物质混合物（S）的第二分离路径（4） 第二分离路径（4）。 第二分离路径（4）相对于第一分离路径（2）以角度（α）倾斜。 第一（2）和第二（4）分离路径的交叉区域形成用于将部分分离的物质混合物（S）注入第二载体介质（E）的第二注入装置（5）。 将流体物质混合物（S）注入到第一注射装置（3）中的载体介质（C）中，然后沿着沿着第一分离路径（2）施加的电场分离。 然后将部分分离的物质混合物（S）注入到第一分离路径（2）下游的第二载体介质（E）中，并且在沿着第二分离路径（4）施加的电场中进一步分离成其组分。