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1.发明授权Method for controlling sample introduction in microcolumn separation techniques and sampling device 失效微柱分离技术和取样装置中样品引入的控制方法公开(公告)号：US06706164B2
公开(公告)日：2004-03-16
申请号：US09737120
申请日：2000-12-13
IPC分类号： G01N27447
CPC分类号： C07K1/26 , G01N27/44743 , G01N27/44791
摘要： In a method for controlling sample introduction in microcolumn separation techniques, more particularly in capillary electrophoresis (CE), where a sample is injected as a sample plug into a sampling device which comprises at least a channel for the electrolyte buffer and a supply and drain channel for the sample. The supply and drain channels discharge into the electrolyte channel at respective supply and drain ports. The distance between the supply port and the drain port geometrically defines a sample volume. The injection of the sample plug into the electrolyte channel is accomplished electrokinetically by applying an electric field across the supply and drain channels for a time at least long enough that the sample component having the lowest electrophoretic mobility is contained within the geometrically defined volume. The supply and drain channels each are inclined to the electrolyte channel. Means are provided for electrokinetically injecting the sample into the sample volume. The resistance to flow of the supply and drain channels with respect to the electrolyte buffer is at least about 5% lower than the respective resistance to flow of the electrolyte channel.
摘要翻译： 在用于控制微柱分离技术中的样品引入的方法中，更具体地在毛细管电泳（CE）中，其中将样品作为样品塞注入采样装置中，所述采样装置至少包括用于电解质缓冲液的通道和供排水通道 为样品。 供应和排出通道在相应的供排水口排放到电解液通道中。 供应端口和排水口之间的距离几何定义了样品体积。 通过在供电和漏极通道上施加电场至少足够长的时间使得具有最低电泳迁移率的样品组分包含在几何限定的体积内，电动地将样品塞注入电解质通道。 供应和排出通道各自倾斜于电解质通道。 提供了用于将样品电动注入样品体积的装置。 供电和排出通道相对于电解质缓冲液的流动阻力比电解液通道的相应阻力低至少约5％。
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2.发明授权公开(公告)号：US06423198B1
公开(公告)日：2002-07-23
申请号：US09657772
申请日：2000-09-08
IPC分类号： G01N2726
CPC分类号： C07K1/26 , G01N27/44743 , G01N27/44791
摘要： In a method for controlling sample introduction in microcolumn separation techniques, more particularly in capillary electrophoresis (CE), where a sample is injected as a sample plug into a sampling device which comprises at least a channel for the electrolyte buffer and a supply and drain channel for the sample. The supply and drain channels discharge into the electolyte channel at respective supply and drain ports. The distance between the supply port and the drain port geometrically defines a sample volume. The injection of the sample plug into the electrolyte channel is accomplished electrokinetically by applying an electric field across the supply and drain channels for a time at least long enough that the sample component having the lowest electrophoretic mobility is contained within the geometrically defined volume. The supply and drain channels each are inclined to the electrolyte channel. Means are provided for electrokinetically injecting the sample into the sample volume. The resistance to flow of the source and drain channels with respect to the electrolyte buffer is at least about 5% lower than the respective resistance to flow of the electrolyte channel.
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3.发明授权Optical detection arrangement for small volume chemical analysis of fluid samples 失效流体样品小体积化学分析光学检测装置
公开(公告)号：US5636017A
公开(公告)日：1997-06-03
申请号：US212521
申请日：1994-03-11
申请人： Alfredo E. Bruno , Beat Krattiger , Carlo S. Effenhauser , Francois Maystre , Philippe Nussbaum
发明人： Alfredo E. Bruno , Beat Krattiger , Carlo S. Effenhauser , Francois Maystre , Philippe Nussbaum
IPC分类号： G01N21/45 , G01N21/05 , G01N21/64 , G01N27/447 , G01N30/74
CPC分类号： G01N30/74 , G01N21/05 , G01N27/44721 , G01N2021/0346
摘要： An optical detection arrangement for small volume chemical analysis comprises a light source (22), a capillary tube (23) and a photoelectric detector (24). The arrangement of the light source (22) relative to the capillary tube (23) is such, that probing light (P) emitted from the light source (22) strikes a sample (S) to be analyzed, which is flowing through the capillary tube (23), whereas the photoelectric detector (24) is arranged relative to the capillary tube (23) such, that it is capable of detecting light comming from the capillary tube. The photoelectric detector (24) is connected with an evaluation electronics. Between the light source (22) and the capillary tube (23)the probing light (P) is guided essentially in a guiding means (26) which is made of a material having a refractive index gradient about perpendicular to the direction of propagation of the probing light (P), and which is connected to the capillary tube (23) such that probing light (P) exiting the guiding material (26) strikes the capillary tube (23). Preferably light emitting diodes (LEDs) or laser diodes are used as light source (22).
摘要翻译： 用于小容量化学分析的光学检测装置包括光源（22），毛细管（23）和光电检测器（24）。 光源（22）相对于毛细管（23）的布置是这样的，即从光源（22）发射的探测光（P）撞击待分析的样品（S），该样品（S）流过毛细管 管（23），而光电检测器（24）相对于毛细管（23）布置，使得其能够检测来自毛细管的光。 光电检测器（24）与评估电路连接。 在光源（22）和毛细管（23）之间，探测光（P）基本上在引导装置（26）中引导，该引导装置（26）由具有垂直于 探测光（P），并且其连接到毛细管（23），使得离开引导材料（26）的探测光（P）撞击毛细管（23）。 优选使用发光二极管（LED）或激光二极管作为光源（22）。
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4.发明授权Microfluidic device for controlling sample introduction in microcolumn separation techniques and sampling device 失效用于控制微柱分离技术和取样装置中样品引入的微流体装置公开(公告)号：US07691245B2
公开(公告)日：2010-04-06
申请号：US11316414
申请日：2005-12-21
IPC分类号： G01N27/447 , G01N27/453
CPC分类号： C07K1/26 , G01N27/44743 , G01N27/44791
摘要： A microfluidic device for controlling sample introduction in microcolumn separation techniques, more particularly in capillary electrophoresis (CE), where a sample is injected as a sample plug into a sampling device which comprises at least a channel for the electrolyte buffer and a supply and drain channel for the sample. The supply and drain channels discharge into the electrolyte channel at respective supply and drain ports. The distance between the supply port and the drain port geometrically defines a sample volume. The injection of the sample plug into the electrolyte channel is accomplished electrokinetically by applying an electric field across the supply and drain channels for a time at least long enough that the sample component having the lowest electrophoretic mobility is contained within the geometrically defined volume. Means are provided for electrokinetically injecting the sample into the sample volume.
摘要翻译： 一种用于控制微柱分离技术中的样品引入的微流体装置，更具体地在毛细管电泳（CE）中，其中将样品作为样品塞注入采样装置，该采样装置至少包括用于电解质缓冲液的通道和供排水通道 为样品。 供应和排出通道在相应的供排水口排放到电解液通道中。 供应端口和排水口之间的距离几何定义了样品体积。 通过在供电和漏极通道上施加电场至少足够长的时间使得具有最低电泳迁移率的样品组分包含在几何限定的体积内，电动地将样品塞注入电解质通道。 提供了用于将样品电动注入样品体积的装置。
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5.发明授权Method for controlling sample introduction in microcolumn separation techniques and sampling device 有权微柱分离技术和取样装置中样品引入的控制方法公开(公告)号：US06491804B2
公开(公告)日：2002-12-10
申请号：US09775021
申请日：2001-01-31
IPC分类号： C25B700
CPC分类号： C07K1/26 , G01N27/44743 , G01N27/44791
摘要： In a method for controlling sample introduction in microcolumn separation techniques, more particularly in capillary electrophoresis (CE), where a sample is injected as a sample plug into a sampling device which comprises at least a channel for the electrolyte buffer and a supply and drain channel for the sample. The supply and drain channels discharge into the electolyte channel at respective supply and drain ports. The distance between the supply port and the drain port geometrically defines a sample volume. The injection of the sample plug into the electrolyte channel is accomplished electrokinetically by applying an electric field across the supply and drain channels for a time at least long enough that the sample component having the lowest electrophoretic mobility is contained within the geometrically defined volume. The supply and drain channels each are inclined to the electrolyte channel. Means are provided for electrokinetically injecting the sample into the sample volume. The resistance to flow of the source and drain channels with respect to the electrolyte buffer is at least about 5% lower than the respective resistance to flow of the electrolyte channel.
摘要翻译： 在用于控制微柱分离技术中的样品引入的方法中，更具体地在毛细管电泳（CE）中，其中将样品作为样品塞注入采样装置中，所述采样装置至少包括用于电解质缓冲液的通道和供排水通道 为样品。 供电和排出通道在相应的供排水口排放到电极通道中。 供应端口和排水口之间的距离几何定义了样品体积。 通过在供电和漏极通道上施加电场至少足够长的时间使得具有最低电泳迁移率的样品组分包含在几何限定的体积内，电动地将样品塞注入电解质通道。 供应和排出通道各自倾斜于电解质通道。 提供了用于将样品电动注入样品体积的装置。 源极和漏极通道相对于电解质缓冲液的流动阻力比电解质通道的相应阻力低至少约5％。
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6.发明授权Method for controlling sample introduction in microcolumn separation techniques and sampling device 失效微柱分离技术和取样装置中样品引入的控制方法公开(公告)号：US06730202B2
公开(公告)日：2004-05-04
申请号：US09780849
申请日：2001-02-09
IPC分类号： C25B700
CPC分类号： C07K1/26 , G01N27/44743 , G01N27/44791
摘要： In a method for controlling sample introduction in microcolumn separation techniques, more particularly in capillary electrophoresis (CRE), where a sample is injected as a sample plug into a sampling device which comprises at least a channel for the electrolyte buffer and a supply and drain channel for the sample. The supply and drain channels discharge into the electrolyte channel at respective supply and drain ports. The distance between the supply port and the drain port geometrically defines a sample volume. The injection of the sample plug into the electrolyte channel is accomplished electrokinetically by applying an electric field across the supply and drain channels for a time at least long enough that the sample component having the lowest electrophoretic mobility is contained within the geometrically defined volume. The supply and drain channels each are inclined to the electrolyte channel. Means are provided for electrokinetically injecting the sample into the sample volume. The resistance to flow of the source and drain channels with respect to the electrolyte buffer is at least about 5% lower than the respective resistance to flow of the electrolyte channel.
摘要翻译： 在用于控制微柱分离技术中的样品引入的方法中，更特别地在毛细管电泳（CRE）中，其中将样品作为样品塞注入采样装置中，该采样装置至少包括用于电解质缓冲液的通道和供排水通道 为样品。 供应和排出通道在相应的供排水口排放到电解液通道中。 供应端口和排水口之间的距离几何定义了样品体积。 通过在供电和漏极通道上施加电场至少足够长的时间使得具有最低电泳迁移率的样品组分包含在几何限定的体积内，电动地将样品塞注入电解质通道。 供应和排出通道各自倾斜于电解质通道。 提供了用于将样品电动注入样品体积的装置。 源极和漏极通道相对于电解质缓冲液的流动阻力比电解质通道的相应阻力低至少约5％。
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7.发明授权Method for controlling sample introduction in microcolumn separation techniques and sampling device 有权微柱分离技术和取样装置中样品引入的控制方法公开(公告)号：US06699378B2
公开(公告)日：2004-03-02
申请号：US09747767
申请日：2000-12-22
IPC分类号： C25B1500
CPC分类号： C07K1/26 , G01N27/44743 , G01N27/44791
摘要： In a method for controlling sample introduction in microcolumn separation techniques, more particularly in capillary electrophoresis (CE), where a sample is injected as a sample plug into a sampling device which comprises at least a channel for the electrolyte buffer and a supply and drain channel for the sample. The supply and drain channels discharge into the electrolyte channel at respective supply and drain ports. The distance between the supply port and the drain port geometrically defines a sample volume. The injection of the sample plug into the electrolyte channel is accomplished electrokinetically by applying an electric field across the supply and drain channels for a time at least long enough that the sample component having the lowest electrophoretic mobility is contained within the geometrically defined volume. The supply and drain channels each are inclined to the electrolyte channel. Means are provided for electrokinetically injecting the sample into the sample volume. The resistance to flow of the source and drain channels with respect to the electrolyte buffer is at least about 5% lower than the respective resistance to flow of the electrolyte channel.
摘要翻译： 在用于控制微柱分离技术中的样品引入的方法中，更具体地在毛细管电泳（CE）中，其中将样品作为样品塞注入采样装置中，所述采样装置至少包括用于电解质缓冲液的通道和供排水通道 为样品。 供应和排出通道在相应的供排水口排放到电解液通道中。 供应端口和排水口之间的距离几何定义了样品体积。 通过在供电和漏极通道上施加电场至少足够长的时间使得具有最低电泳迁移率的样品组分包含在几何限定的体积内，电动地将样品塞注入电解质通道。 供应和排出通道各自倾斜于电解质通道。 提供了用于将样品电动注入样品体积的装置。 源极和漏极通道相对于电解质缓冲液的流动阻力比电解质通道的相应阻力低至少约5％。
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8.发明授权Method for controlling sample introduction in microcolumn separation techniques and sampling device 失效微柱分离技术和取样装置中样品引入的控制方法公开(公告)号：US06699377B2
公开(公告)日：2004-03-02
申请号：US09747754
申请日：2000-12-22
IPC分类号： C25B700
CPC分类号： C07K1/26 , G01N27/44743 , G01N27/44791
摘要： In a method for controlling sample introduction in microcolumn separation techniques, more particularly in capillary electrophoresis (CE), where a sample is injected as a sample plug into a sampling device which comprises at least a channel for the electrolyte buffer and a supply and drain channel for the sample. The supply and drain channels discharge into the electolyte channel at respective supply and drain ports. The distance between the supply port and the drain port geometrically defines a sample volume. The injection of the sample plug into the electrolyte channel is accomplished electrokinetically by applying an electric field across the supply and drain channels for a time at least long enough that the sample component having the lowest electrophoretic mobility is contained within the geometrically defined volume. The supply and drain channels each are inclined to the electrolyte channel. Means are provided for electrokinetically injecting the sample into the sample volume. The resistance to flow of the source and drain channels with respect to the electrolyte buffer is at least about 5% lower than the respective resistance to flow of the electrolyte channel.
摘要翻译： 在用于控制微柱分离技术中的样品引入的方法中，更具体地在毛细管电泳（CE）中，其中将样品作为样品塞注入采样装置中，所述采样装置至少包括用于电解质缓冲液的通道和供排水通道 为样品。 供电和排出通道在相应的供排水口排放到电极通道中。 供应端口和排水口之间的距离几何定义了样品体积。 通过在供电和漏极通道上施加电场至少足够长的时间使得具有最低电泳迁移率的样品组分包含在几何限定的体积内，电动地将样品塞注入电解质通道。 供应和排出通道各自倾斜于电解质通道。 提供了用于将样品电动注入样品体积的装置。 源极和漏极通道相对于电解质缓冲液的流动阻力比电解质通道的相应阻力低至少约5％。
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9.发明授权Method for controlling sample introduction in microcolumn separation techniques and sampling device 失效微柱分离技术和取样装置中样品引入的控制方法公开(公告)号：US06960286B2
公开(公告)日：2005-11-01
申请号：US09780230
申请日：2001-02-09
CPC分类号： C07K1/26 , G01N27/44743 , G01N27/44791
摘要： In a method for controlling sample introduction in microcolumn separation techniques, more particularly in capillary electrophoresis (CE), where a sample is injected as a sample plug into a sampling device which comprises at least a channel for the electrolyte buffer and a supply and drain channel for the sample. The supply and drain channels discharge into the electrolyte channel at respective supply and drain ports. The distance between the supply port and the drain port geometrically defines a sample volume. The injection of the sample plug into the electrolyte channel is accomplished electrokinetically by applying an electric field across the supply and drain channels for a time at least long enough that the sample component having the lowest electrophoretic mobility is contained within the geometrically defined volume. The supply and drain channels each are inclined to the electrolyte channel. Means are provided for electrokinetically injecting the sample into the sample volume. The resistance to flow of the source and drain channels with respect to the electrolyte buffer is at least about 5% lower than the respective resistance to flow of the electrolyte channel.
摘要翻译： 在用于控制微柱分离技术中的样品引入的方法中，更具体地在毛细管电泳（CE）中，其中将样品作为样品塞注入采样装置中，所述采样装置至少包括用于电解质缓冲液的通道和供排水通道 为样品。 供应和排出通道在相应的供排水口排放到电解液通道中。 供应端口和排水口之间的距离几何定义了样品体积。 通过在供电和漏极通道上施加电场至少足够长的时间使得具有最低电泳迁移率的样品组分包含在几何限定的体积内，电动地将样品塞注入电解质通道。 供应和排出通道各自倾斜于电解质通道。 提供了用于将样品电动注入样品体积的装置。 源极和漏极通道相对于电解质缓冲液的流动阻力比电解质通道的相应阻力低至少约5％。
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10.发明授权公开(公告)号：US06280589B1
公开(公告)日：2001-08-28
申请号：US08226605
申请日：1994-04-12
IPC分类号： C25B700
CPC分类号： C07K1/26 , G01N27/44743 , G01N27/44791
摘要： In a method for controlling sample introduction in microcolumn separation techniques, more particularly in capillary electrophoresis (CE), where a sample is injected as a sample plug into a sampling device which comprises at least a channel for the electrolyte buffer and a supply and drain channel for the sample. The supply and drain channels discharge into the electolyte channel at respective supply and drain ports. The distance between the supply port and the drain port geometrically defines a sample volume. The injection of the sample plug into the electrolyte channel is accomplished electrokinetically by applying an electric field across the supply and drain channels for a time at least long enough that the sample component having the lowest electrophoretic mobility is contained within the geometrically defined volume. The supply and drain channels each are inclined to the electrolyte channel. Means are provided for electrokinetically injecting the sample into the sample volume. The resistance to flow of the source and drain channels with respect to the electrolyte buffer is at least about 5% lower than the respective resistance to flow of the electrolyte channel.
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