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    MULTI-CHAIN EUKARYOTIC DISPLAY VECTORS AND USES THEREOF
    15.
    发明申请
    MULTI-CHAIN EUKARYOTIC DISPLAY VECTORS AND USES THEREOF 有权
    多链显示矢量及其用途

    公开(公告)号：US20130331289A1

    公开(公告)日：2013-12-12

    申请号：US13937915

    申请日：2013-07-09

    Applicant: Dyax Corp.

    Inventor： Simon E. Hufton ,  Hendricus Renerus Jacobus Mattheus Hoogenboom

    IPC: G01N33/554

    CPC classification number: C12N15/1037 ,  C12N15/85 ,  C12N2795/00043 ,  C12N2800/108 ,  C12N2830/002 ,  C12N2840/20 ,  C40B40/02 ,  G01N33/554

    Abstract: A eukaryotic expression vector capable of displaying a multi-chain polypeptide on the surface of a host cell is provided, such that the biological activity of the multi-chain polypeptide is exhibited at the surface of the host cell. Such a vector allows for the display of complex biologically active polypeptides, e.g., biologically active multi-chain polypeptides such as immunoglobulin Fab fragments. The present invention describes and enables the successful display of a multi-chain polypeptide on the surface of a eukaryotic host cell. Preferred vectors are described for expressing the chains of a multi-chain polypeptide in a host cell separately and independently (e.g., under separate vector control elements, and/or on separate expression vectors, thus forming a matched vector set). The use of such matched vector sets provides flexibility and versatility in the generation of eukaryotic display libraries, for example the ability to generate and to display multi-chain polypeptides by combining and recombining vectors that express variegations of the individual chains of a multi-chain polypeptide. Entire repertoires of novel chain combinations can be devised using such vector sets.

    Abstract translation: 提供能够在宿主细胞表面上显示多链多肽的真核表达载体，使得多链多肽的生物学活性显示在宿主细胞的表面。 这种载体允许显示复杂的生物活性多肽，例如生物活性多链多肽，例如免疫球蛋白Fab片段。 本发明描述并使得能够在真核宿主细胞的表面上成功显示多链多肽。 描述了优选的载体，用于在宿主细胞中分别且独立地（例如，在单独的载体控制元件下和/或在分开的表达载体上，从而形成匹配的载体集）表达多链多肽的链。 使用这种匹配的载体集提供了在真核生物显示文库的产生中的灵活性和多功能性，例如通过组合和重组表达多链多肽的各个链的多样性的载体来产生和显示多链多肽的能力 。 可以使用这样的矢量集来设计新的链组合的全部曲目。

    ANTIBODIES AGAINST PHOSPHORYLCHOLINE
    16.
    发明申请
    ANTIBODIES AGAINST PHOSPHORYLCHOLINE 有权

    公开(公告)号：US20210238311A1

    公开(公告)日：2021-08-05

    申请号：US17229837

    申请日：2021-04-13

    Applicant: Athera Biotechnologies AB ,  Dyax Corp.

    Inventor： Knut PETTERSSON ,  Ola CAMBER ,  Dan SEXTON ,  Andrew E. NIXON

    IPC: C07K16/44

    Abstract: The present invention relates to an antibody or antibody fragment capable of binding to phosphorylcholine and/or a phosphorylcholine conjugate, wherein the antibody or antibody fragment comprises a variable heavy chain (VH) domain and/or a variable light chain (VL) domain, and wherein— (a) the VH domain comprises an amino acid sequence that includes one, two or three complementarity determining regions (CDRs) selected from the group consisting of: a CDR1 sequence comprising an amino acid sequence having at least 25%, 50%, 75% or 100% sequence identity to the sequence of SEQ ID NO: 17; a CDR2 sequence comprising an amino acid sequence having at least 5%, 11%, 17%, 23%, 29%, 35%, 47%, 52%, 58%, 64%, 70%, 76%, 82%, 94% or 100% sequence identity to the sequence of SEQ ID NO: 18; and a CDR3 sequence comprising an amino acid sequence having at least 4%, 9%, 13%, 18%, 22%, 27%, 31%, 36%, 40%, 45%, 50%, 54%, 59%, 63%, 68%, 72%, 77%, 81%, 86%, 90%, 95% or 100% sequence identity to the sequence of SEQ ID NO: 19, 20, 21 or 22; and/or (b) the VL domain comprises an amino acid sequence that includes one, two or three complementarity determining regions (CDRs) selected from the group consisting of: a CDR4 sequence comprising an amino acid sequence having at least 5%, 11%, 17%, 23%, 29%, 35%, 47%, 52%, 58%, 64%, 70%, 76%, 82%, 94% or 100% sequence identity to the sequence of SEQ ID NO: 23 or 24; a CDR5 sequence comprising an amino acid sequence having at least 14%, 28%, 42%, 57%, 71%, 85% or 100% sequence identity to the sequence of SEQ ID NO: 25; a CDR6 sequence comprising an amino acid sequence having at least 11%, 22%, 33%, 44%, 55%, 66%, 77%, 88% or 100% sequence identity to the sequence of SEQ ID NO: 26.

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