公开(公告)号：US20210132090A1

公开(公告)日：2021-05-06

申请号：US17143463

申请日：2021-01-07

Applicant: Cleveland Heartlab, Inc.

Inventor： Cory Bystrom ,  Timothy Collier

IPC: G01N33/92

Abstract: Provided herein are methods, systems, and compositions for detecting one or more HDL-associated proteins (e.g., ApoC3; ApoC3 and ApoA1; ApoC3 and SAA1/2; or proteins in Biomarker Panels 1-30) in a sample from a subject with, or suspected of having, cardiovascular disease (CVD) or other HDL related disease. In certain embodiments, such methods, systems, and compositions are used to determine the approximate risk of CVD (or other disease) for a subject, and/or the approximate cholesterol efflux capacity (CEC) of a sample.

公开(公告)号：US20150376704A1

公开(公告)日：2015-12-31

申请号：US14788828

申请日：2015-07-01

Applicant: CLEVELAND HEARTLAB, INC.

Inventor： Doug Harrington ,  Evangelos Hytopoulos ,  Bruce Phelps

IPC: C12Q1/68 ,  G01N33/68 ,  G06F19/00

CPC classification number: C12Q1/6883 ,  C12Q2600/106 ,  C12Q2600/112 ,  C12Q2600/118 ,  C12Q2600/158 ,  C12Q2600/178 ,  G01N33/6893 ,  G01N2570/00 ,  G01N2800/32 ,  G01N2800/50 ,  G01N2800/60 ,  G16B20/00 ,  G16B40/00 ,  G16H50/20

Abstract: The disclosed methods, assays and kits identify biomarkers, particularly miRNA and/or protein biomarkers, for assessing the cardiovascular health of a human. In certain embodiments, methods, assays and kits, circulating miRNA and/or protein biomarkers are identified for assessing the cardiovascular health of a human.

Abstract translation: 所公开的方法，测定和试剂盒鉴定用于评估人的心血管健康的生物标志物，特别是miRNA和/或蛋白质生物标志物。 在某些实施方案中，鉴定了用于评估人的心血管健康的方法，测定和试剂盒，循环的miRNA和/或蛋白质生物标志物。

公开(公告)号：US20150331000A1

公开(公告)日：2015-11-19

申请号：US14713046

申请日：2015-05-15

Applicant: CLEVELAND HEARTLAB, INC.

Inventor： Timothy Collier ,  Cory Bystrom ,  Angela Higgins

IPC: G01N33/92 ,  G01N1/34

CPC classification number: G01N33/92 ,  C07K14/775 ,  G01N1/34

Abstract: The present invention provides methods, kits, and compositions for purifying HDL molecules from a sample (e.g., blood sample) using HDL tagging molecules comprising an HDL lipophilic core binding peptide (e.g., portion of ApoA1) and an affinity tag. The present invention also provides methods, kits, and compositions for detecting non-fragmented ApoA1 with mass spectrometry. The present invention further provides methods, kits, and compositions for tagging HDL molecules in a sample with detectably labeled ApoA1 molecules such that the ratio of detectably labeled ApoA1 molecules to native ApoA1 proteins may be determined.

Abstract translation: 本发明提供了使用包含HDL亲脂性核心结合肽（例如，ApoA1的部分）和亲和标签的HDL标记分子从样品（例如血液样品）中纯化HDL分子的方法，试剂盒和组合物。 本发明还提供用质谱法检测非碎片化的ApoA1的方法，试剂盒和组合物。 本发明还提供用于标记具有可检测标记的ApoA1分子的样品中的HDL分子的方法，试剂盒和组合物，使得可测定可检测标记的ApoA1分子与天然ApoA1蛋白的比例。

公开(公告)号：US11237167B2

公开(公告)日：2022-02-01

申请号：US15874078

申请日：2018-01-18

Applicant: Cleveland HeartLab, Inc.

Inventor： Celalettin Topbas ,  Cory Bystrom

IPC: G01N33/573 ,  G01N1/28

Abstract: Provided herein are methods, systems, and compositions for Lp-PLA2 detection assays that employ amounts of detergent to liberate all or nearly all of the Lp-PLA2 molecules from associated lipoprotein particles. In this regard, the true Lp-PLA2 concentration can be detected in a sample, which correlates better with known Lp-PLA2 activity assays.

公开(公告)号：US10151764B2

公开(公告)日：2018-12-11

申请号：US14713046

申请日：2015-05-15

Applicant: CLEVELAND HEARTLAB, INC.

Inventor： Timothy Collier ,  Cory Bystrom ,  Angela Higgins

IPC: G01N33/483 ,  G01N33/92 ,  G01N1/34 ,  C07K14/775

Abstract: The present invention provides methods, kits, and compositions for purifying HDL molecules from a sample (e.g., blood sample) using HDL tagging molecules comprising an HDL lipophilic core binding peptide (e.g., portion of ApoA1) and an affinity tag. The present invention also provides methods, kits, and compositions for detecting non-fragmented ApoA1 with mass spectrometry. The present invention further provides methods, kits, and compositions for tagging HDL molecules in a sample with detectably labeled ApoA1 molecules such that the ratio of detectably labeled ApoA1 molecules to native ApoA1 proteins may be determined.

公开(公告)号：US20180209977A1

公开(公告)日：2018-07-26

申请号：US15874078

申请日：2018-01-18

Applicant: Cleveland HeartLab, Inc.

Inventor： Celalettin Topbas ,  Cory Bystrom

IPC: G01N33/573

CPC classification number: G01N33/573 ,  G01N1/28 ,  G01N2333/92 ,  G01N2800/32

Abstract: Provided herein are methods, systems, and compositions for Lp-PLA2 detection assays that employ amounts of detergent to liberate all or nearly all of the Lp-PLA2 molecules from associated lipoprotein particles. In this regard, the true Lp-PLA2 concentration can be detected in a sample, which correlates better with known Lp-PLA2 activity assays.

公开(公告)号：US20180024152A1

公开(公告)日：2018-01-25

申请号：US15656592

申请日：2017-07-21

Applicant: Cleveland HeartLab, Inc.

Inventor： Cory Bystrom ,  Timothy Collier

IPC: G01N33/92

Abstract: Provided herein are methods, systems, and compositions for detecting one or more HDL-associated proteins (e.g., ApoC3; ApoC3 and ApoA1; ApoC3 and SAA1/2; or proteins in Biomarker Panels 1-30) in a sample from a subject with, or suspected of having, cardiovascular disease (CVD) or other HDL related disease. In certain embodiments, such methods, systems, and compositions are used to determine the approximate risk of CVD (or other disease) for a subject, and/or the approximate cholesterol efflux capacity (CEC) of a sample.

公开(公告)号：US09810702B2

公开(公告)日：2017-11-07

申请号：US14858111

申请日：2015-09-18

Applicant: Cleveland HeartLab, Inc.

Inventor： Cory Bystrom ,  Timothy Collier

IPC: G01N33/92

CPC classification number: G01N33/92 ,  G01N2333/775

Abstract: Provided herein are compositions, systems, and methods for extracting and detecting at least one HDL-associated protein (e.g., ApoA1) from a sample (e.g., plasma or serum sample). In certain embodiments, a strong organic acid and hydrophilic organic solvent are mixed with the sample; after centrifugation, the supernatant is transferred to a second container where it is mixed with a non-polar organic solvent; after centrifugation, the lower aqueous layer is transferred to a third container; and then at least a portion of the transferred aqueous layer is subjected to a detection assay such that at least one HDL-associated protein is detected.

公开(公告)号：US20030194812A1

公开(公告)日：2003-10-16

申请号：US10122081

申请日：2002-04-12

Applicant: Berkeley HeartLab, Inc.

Inventor： David Shewmake ,  Frank Ruderman ,  Christopher Boggess

IPC: G01N033/92

CPC classification number: G01N33/92 ,  G01N2800/32 ,  G01N2800/52

Abstract: The invention provides a method for identifying patients with normal NCEP lipid levels who are in need of treatment for cardiovascular disease comprising measuring one or more LDL or HDL particle subclass levels and identifying abnormal LDL or HDL subclass levels. LDL III anullb and HDL 2b are preferred subclasses.

Abstract translation: 本发明提供了一种用于鉴定需要治疗心血管疾病的正常NCEP脂质水平的患者的方法，包括测量一种或多种LDL或HDL颗粒亚类水平并鉴定异常的LDL或HDL亚类水平。 LDL III a + b和HDL 2b是优选的亚类。

公开(公告)号：US20020046215A1

公开(公告)日：2002-04-18

申请号：US09920973

申请日：2001-08-02

Applicant: HEARTLAB, INC.

Inventor： Robert R. Petrocelli

IPC: G06F012/00

CPC classification number: G06F19/321 ,  G06F19/00 ,  Y10S707/99945 ,  Y10S707/99955

Abstract: An archive system is provided for long term storage of large amounts of data that is particularly suited for multi-year storage of medical data such as cardiac images, patient demographics and reports. One or more digital versatile disks (DVDs) may be employed as the storage media. In the archival process, all of the data for a particular patient, procedure or study is stored and uniquely identified within one DVD. Each DVD may have an executable program stored thereon for independently accessing the archived data from the selected DVD. Before the DVD is recorded, the data to be archived is segmented into a plurality of information groups where each group is based on data for the particular patient, procedure or study. Then, the DVD is reviewed to determine whether a sufficient amount of storage space is present to ensure that the information group will be entirely stored within one DVD.

Abstract translation: 提供存档系统用于长期存储大量数据，特别适用于诸如心脏图像，患者人口统计和报告的医疗数据的多年存储。 可以使用一个或多个数字通用盘（DVD）作为存储介质。 在归档过程中，特定患者，程序或研究的所有数据在一张DVD内存储和唯一标识。 每个DVD可以具有存储在其上的可执行程序，用于独立地从所选择的DVD访问归档数据。 在记录DVD之前，要归档的数据被分割成多个信息组，其中每个组基于特定患者，程序或研究的数据。 然后，检查DVD以确定是否存在足够的存储空间以确保信息组将完全存储在一个DVD内。