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公开(公告)号：US20170145482A1
公开(公告)日：2017-05-25
申请号：US15300183
申请日：2015-03-27
申请人： RIKEN , KABUSHIKI KAISHA DNAFORM
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6818 , C09B23/04 , C09B23/06 , C12Q1/6816 , C12Q2563/107 , C12Q2565/1015 , C12Q2523/31 , C12Q2563/113
摘要： The present invention is intended to provide a novel fluorescent labeled single-stranded nucleic acid, by which the background of an exciton oligomer can be further reduced and the novel use thereof. The present invention relates to a labeled single-stranded nucleic acid having at least two fluorescent atomic group pairs that exhibit an exciton effect. The labeled single-stranded nucleic acid is characterized in that the emission peak wavelength of one of the fluorescent atomic group pairs (fluorescent atomic group pair A) is shorter than the excitation peak wavelength of the other fluorescent atomic group pair (fluorescent atomic group pair B), and the fluorescent atomic group pairs A and B have a Förster resonance energy transfer (FRET) effect. This fluorescent labeled single-stranded nucleic acid is usable as a primer for amplifying a target nucleic acid or a probe to be hybridized with a target nucleic acid.
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公开(公告)号：US10294261B2
公开(公告)日：2019-05-21
申请号：US14424826
申请日：2013-09-03
IPC分类号： C07H19/06 , C07H21/04 , C09B23/04 , C09B23/06 , C07H19/073 , C12Q1/6876
摘要： The present invention provides a compound represented by the following chemical formula (I); a tautomer or stereoisomer of the compound; or a salt of the compound, the tautomer, or the stereoisomer. In the chemical formula (I), R1 and R2 are each a Group 1 element or a protecting group of an amino group and may be identical to or different from each other, or alternatively, R1 and R2 together may form a protecting group of an amino group. R3 is a Group 1 element or a protecting group of a hydroxy group. R4 is a Group 1 element or —PR5R6R7R8 (R5, R6, R7, and R8 are each a Group 1 element, a lone electron pair, a Group 16 element, a Group 17 element, or a protecting group of a phosphorus atom, and may be identical to or different from each other). J is a hydrogen atom or an arbitrary atomic group, A is a hydrogen atom, a hydroxy group, an alkyl group, an aralkyl group, an alkoxy group, an electron-withdrawing group, a silylene group, or a sulfide group, or alternatively, J and A together may form a linker. L is a single bond or a linker (a linking atom or a linking atomic group), the main chain length (the number of main chain atoms) of the linker is arbitrary, L may or may not contain each of C, N, O, S, P, and Si in the main chain, and may or may not contain each of a single bond, a double bond, a triple bond, an amide bond, an ester bond, a disulfide bond, an imino group, an ether bond, a thioether bond, and a thioester bond in the main chain. Z is an atomic group that can form a peptide bond with a labeling compound, or is an atom or atomic group including a label.
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公开(公告)号：US20190099758A1
公开(公告)日：2019-04-04
申请号：US16086875
申请日：2017-12-22
发明人： Koichiro TSUJIMARU
摘要： The present invention provides a new smaller analysis device that can heat an analysis chip. The analysis device includes a set section in which an analysis chip is to be set; a housing chamber that accommodates the set section; a heating section that heats the analysis chip set in the set section; and an analysis section that analyzes the analysis chip set in the set section, wherein the housing chamber serves as an analysis chamber of the analysis chip set in the set section in the state where the set section is accommodated in the housing chamber, the analysis section is disposed at a position where the analysis chip set in the set section is analyzed in the state where the set section is accommodated in the housing chamber, and the heating section is a heating plate and is disposed at a position where the analysis chip set in the set section is heated in the state where the set section is accommodated in the housing chamber.
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14.发明授权公开(公告)号：US09862989B2
公开(公告)日：2018-01-09
申请号：US14414806
申请日：2013-07-12
发明人： Yoshihide Hayashizaki , Takeshi Hanami , Takahiro Soma , Yasumasa Kimura , Hajime Kanamori , Yasumasa Mitani
CPC分类号： C12Q1/6818 , C12N2330/30 , C12Q1/6827 , C12Q2525/117 , C12Q2535/131 , C12Q2537/101 , C12Q2563/107 , C12Q2565/1015 , C12Q2565/107
摘要： The present invention provides a nucleic acid probe that can achieve high detection sensitivity and high specificity in mutation detection, mismatch detection, etc. by the PCR method, a method for designing such a nucleic acid probe, and a method for detecting a target sequence. The nucleic acid probe includes a nucleic acid molecule, and the nucleic acid molecule includes a plurality of fluorescent dye moieties that exhibit an excitonic effect. At least two of the fluorescent dye moieties that exhibit an excitonic effect are bound to the same base or two adjacent bases in the nucleic acid molecule with each fluorescent dye moiety being bound via a linker (a linking atom or a linking atomic group). The extension-side end of the nucleic acid molecule is chemically modified, thereby preventing an extension reaction of the nucleic acid molecule.
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公开(公告)号：US20150252070A1
公开(公告)日：2015-09-10
申请号：US14424826
申请日：2013-09-03
CPC分类号： C07H19/06 , C07H19/073 , C07H21/04 , C09B23/04 , C09B23/06 , C12Q1/6876 , Y02P20/55
摘要： The present invention provides a compound represented by the following chemical formula (I); a tautomer or stereoisomer of the compound; or a salt of the compound, the tautomer, or the stereoisomer. In the chemical formula (I), R1 and R2 are each a Group 1 element or a protecting group of an amino group and may be identical to or different from each other, or alternatively, R1 and R2 together may form a protecting group of an amino group. R3 is a Group 1 element or a protecting group of a hydroxy group. R4 is a Group 1 element or —PR5R6R7R8 (R5, R6, R7, and R8 are each a Group 1 element, a lone electron pair, a Group 16 element, a Group 17 element, or a protecting group of a phosphorus atom, and may be identical to or different from each other). J is a hydrogen atom or an arbitrary atomic group, A is a hydrogen atom, a hydroxy group, an alkyl group, an aralkyl group, an alkoxy group, an electron-withdrawing group, a silylene group, or a sulfide group, or alternatively, J and A together may form a linker. L is a single bond or a linker (a linking atom or a linking atomic group), the main chain length (the number of main chain atoms) of the linker is arbitrary, L may or may not contain each of C, N, O, S, P, and Si in the main chain, and may or may not contain each of a single bond, a double bond, a triple bond, an amide bond, an ester bond, a disulfide bond, an imino group, an ether bond, a thioether bond, and a thioester bond in the main chain. Z is an atomic group that can form a peptide bond with a labeling compound, or is an atom or atomic group including a label.
摘要翻译： 本发明提供由下列化学式（I）表示的化合物。 化合物的互变异构体或立体异构体; 或化合物的盐，互变异构体或立体异构体。 在化学式（I）中，R 1和R 2各自为1族元素或氨基的保护基，并且可以相同或不同，或者，R 1和R 2可以一起形成保护基 氨基。 R3是第1族元素或羟基的保护基。 R4是第1族元素或-PR5R6R7R8（R5，R6，R7和R8分别是第1族元素，单电子对，第16族元素，第17族元素或磷原子的保护基， 可以彼此相同或不同）。 J为氢原子或任意的原子团，A为氢原子，羟基，烷基，芳烷基，烷氧基，吸电子基，亚甲硅烷基或硫醚基，或者 J和A一起可形成连接体。 L是单键或连接体（连接原子或连接原子团），连接体的主链长度（主链原子数）是任意的，L可以含有也可以不含有C，N，O ，S，P和Si，并且可以包含或不包含单键，双键，三键，酰胺键，酯键，二硫键，亚氨基，醚 键，硫醚键和硫酯键。 Z是可以与标记化合物形成肽键的原子团，或是包括标记的原子或原子团。
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16.发明申请NUCLEIC ACID PROBE, METHOD FOR DESIGNING NUCLEIC ACID PROBE, AND METHOD FOR DETECTING TARGET SEQUENCE 有权核酸探针，设计核酸探针的方法和检测目标序列的方法公开(公告)号：US20150203902A1
公开(公告)日：2015-07-23
申请号：US14414806
申请日：2013-07-12
发明人： Yoshihide Hayashizaki , Takeshi Hanami , Takahiro Soma , Yasumasa Kimura , Hajime Kanamori , Yasumasa Mitani
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6818 , C12N2330/30 , C12Q1/6827 , C12Q2525/117 , C12Q2535/131 , C12Q2537/101 , C12Q2563/107 , C12Q2565/1015 , C12Q2565/107
摘要： The present invention provides a nucleic acid probe that can achieve high detection sensitivity and high specificity in mutation detection, mismatch detection, etc. by the PCR method, a method for designing such a nucleic acid probe, and a method for detecting a target sequence. The nucleic acid probe includes a nucleic acid molecule, and the nucleic acid molecule includes a plurality of fluorescent dye moieties that exhibit an excitonic effect. At least two of the fluorescent dye moieties that exhibit an excitonic effect are bound to the same base or two adjacent bases in the nucleic acid molecule with each fluorescent dye moiety being bound via a linker (a linking atom or a linking atomic group). The extension-side end of the nucleic acid molecule is chemically modified, thereby preventing an extension reaction of the nucleic acid molecule.
摘要翻译： 本发明提供了一种核酸探针，其可以通过PCR方法在突变检测，错配检测等方面实现高检测灵敏度和高特异性，设计这种核酸探针的方法和用于检测靶序列的方法。 核酸探针包括核酸分子，并且核酸分子包括表现出激子效应的多个荧光染料部分。 显示出激子效应的至少两个荧光染料部分与核酸分子中的相同碱基或两个相邻碱基结合，每个荧光染料部分通过连接体（连接原子或连接原子团）结合。 化学修饰核酸分子的延伸侧末端，从而防止核酸分子的延伸反应。
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公开(公告)号：US20150152496A1
公开(公告)日：2015-06-04
申请号：US14414324
申请日：2013-08-29
发明人： Yoshihide Hayashizaki , Masayoshi Itoh , Takahiro Arakawa , Kengo Usui , Sotaro Uemura , Yasumasa Mitani
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6876 , C12Q1/682 , C12Q1/6834 , C12Q1/6853 , C12Q2600/158 , C12Q2525/301 , C12Q2565/1015 , C12Q2565/107 , C12Q2565/543
摘要： The present invention is to provide a method for analyzing a target nucleic acid, by which the target nucleic acid can be analyzed rapidly and easily. In order to achieve the above object, the present invention provides a method for analyzing a target nucleic acid in a sample, including the step of: analyzing the target nucleic acid in the sample by bringing the sample into contact with a label and with a primer or probe that can hybridize to the target nucleic acid. The primer or probe is immobilized on a solid phase. The label does not emit light when the primer or probe does not hybridize to the target nucleic acid, whereas the label emits light when the primer or probe has hybridized to the target nucleic acid. The analysis is carried out by detecting the light emitted from the label.
摘要翻译： 本发明提供一种用于分析目标核酸的方法，通过该方法可以快速且容易地分析靶核酸。 为了实现上述目的，本发明提供了一种用于分析样品中的靶核酸的方法，包括以下步骤：通过使样品与标记物接触并用引物分析样品中的靶核酸 或可与靶核酸杂交的探针。 引物或探针固定在固相上。 当引物或探针不与靶核酸杂交时，标记不发光，而当引物或探针与靶核酸杂交时，标记物发光。 通过检测从标签发出的光来进行分析。
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