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11.发明申请ADENO-ASSOCIATED VIRUS (AAV) CLADES, SEQUENCES, VECTORS CONTAINING SAME, AND USES THEREFOR 审中-公开ADENO相关病毒（AAV）分类，序列，包含它们的载体及其用途公开(公告)号：US20110236353A1
公开(公告)日：2011-09-29
申请号：US13023918
申请日：2011-02-09
IPC分类号： A61K48/00 , C12N7/00 , C12N15/63 , C12N1/00 , C12N5/10 , C12N7/01 , A61K38/16 , A61P7/00 , A61P11/00
CPC分类号： A61K48/00 , C07K14/005 , C07K14/705 , C12N7/00 , C12N9/644 , C12N15/86 , C12N2750/14121 , C12N2750/14122 , C12N2750/14142 , C12N2750/14143 , C12N2750/14151 , C12N2830/008 , C12N2830/48 , C12N2830/85 , C12Y304/21022
摘要： Sequences of novel adeno-associated virus capsids and vectors and host cells containing these sequences are provided. Also described are methods of using such host cells and vectors in production of rAAV particles. AAV-mediated delivery of therapeutic and immunogenic genes using the vectors of the invention is also provided.
摘要翻译： 提供新的腺相关病毒衣壳和含有这些序列的载体和宿主细胞的序列。 还描述了在生产rAAV颗粒中使用这种宿主细胞和载体的方法。 还提供使用本发明的载体的AAV介导的治疗和免疫原性基因的递送。
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12.发明授权Adeno-associated virus (AAV) clades, sequences, vectors containing same, and uses therefor 有权腺相关病毒（AAV）进化枝，序列，含有其的载体及其用途公开(公告)号：US07906111B2
公开(公告)日：2011-03-15
申请号：US10573600
申请日：2004-09-30
CPC分类号： A61K48/00 , C07K14/005 , C07K14/705 , C12N7/00 , C12N9/644 , C12N15/86 , C12N2750/14121 , C12N2750/14122 , C12N2750/14142 , C12N2750/14143 , C12N2750/14151 , C12N2830/008 , C12N2830/48 , C12N2830/85 , C12Y304/21022
摘要： Sequences of novel adeno-associated virus capsids and vectors and host cells containing these sequences are provided. Also described are methods of using such host cells and vectors in production of rAAV particles. AAV-mediated delivery of therapeutic and immunogenic genes using the vectors of the invention is also provided.
摘要翻译： 提供新的腺相关病毒衣壳和含有这些序列的载体和宿主细胞的序列。 还描述了在生产rAAV颗粒中使用这种宿主细胞和载体的方法。 还提供使用本发明的载体的AAV介导的治疗和免疫原性基因的递送。
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13.发明申请SIMIAN E ADENOVIRUSES SAdV-39, -25.2, -26, -30, -37, AND -38 有权SIMIAN E ADENOVIRUSES SAdV-39，-25.2，-26，-30，-37和-38公开(公告)号：US20100247490A1
公开(公告)日：2010-09-30
申请号：US12744441
申请日：2008-11-24
IPC分类号： A61K35/76 , C12N7/00 , C12N7/01 , C07H21/04 , C12N15/861 , C07K14/075 , A61K38/16 , A61P31/12 , C12N5/02
CPC分类号： C12N15/86 , A61K48/00 , A61K2039/525 , A61K2039/5256 , C07K14/005 , C12N7/00 , C12N2710/10321 , C12N2710/10322 , C12N2710/10343 , C12N2710/10361 , C12N2760/16122
摘要： A recombinant vector comprises simian adenovirus SAdV-39, -25.2, -26, -30, -37, and -38 sequences and a heterologous gene under the control of regulatory sequences. A cell line which expresses simian adenovirus SAdV-39, -25.2, -26, -30, -37, and -383 gene(s) is also disclosed. Methods of using the vectors and cell lines are provided.
摘要翻译： 重组载体包含猿猴腺病毒SAdV-39，-25.2，-26，-30，-37和-38序列，以及在调控序列控制下的异源基因。 还公开了表达猿猴腺病毒SAdV-39，-25.2，-26，-30，-37和-383基因的细胞系。 提供了使用载体和细胞系的方法。
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14.发明授权Simian adenoviruses SAdV-43, -45, -46, -47, -48, -49, and -50 and uses thereof 有权猿猴腺病毒SAdV-43，-45，-46，-47，-48，-49和-50及其用途公开(公告)号：US08940290B2
公开(公告)日：2015-01-27
申请号：US13126557
申请日：2009-10-29
CPC分类号： C12N15/86 , C12N2710/10321 , C12N2710/10322 , C12N2710/10341 , C12N2710/10342 , C12N2710/10343 , C12N2710/10345 , C12N2710/10352 , C12N2810/6018
摘要： A recombinant vector comprises simian adenovirus 43, 45, 46, 47, 48, 49 or 50 sequences and a heterologous gene under the control of regulatory sequences. A cell line which expresses simian adenovirus 43, 45, 46, 47, 48, 49 or 50 gene(s) is also disclosed. Methods of using the vectors and cell lines are provided.
摘要翻译： 重组载体包含猿猴腺病毒43,45,46,47,48,49或50个序列，以及在调控序列控制下的异源基因。 还公开了表达猿腺病毒43,45,46,47,48,49或50个基因的细胞系。 提供了使用载体和细胞系的方法。
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公开(公告)号：US08846031B2
公开(公告)日：2014-09-30
申请号：US13321985
申请日：2010-05-27
IPC分类号： C12N15/861 , C12N7/01 , C07K14/075 , C12N15/33
CPC分类号： C12N15/86 , A61K39/145 , A61K48/00 , A61K2039/5256 , C07K14/005 , C12N7/00 , C12N2710/10043 , C12N2710/10321 , C12N2710/10322 , C12N2710/10343 , C12N2810/6018
摘要： Novel simian adenovirus 41 and two isolates thereof are described. Various uses of these isolates, including construction of a recombinant vector which comprises simian adenovirus 41 sequences and a heterologous gene under the control of regulatory sequences are provided. A cell line which expresses simian adenovirus 41 gene(s) is also disclosed. Methods of using the vectors and cell lines are provided.
摘要翻译： 描述了新型猿猴腺病毒41及其两个分离物。 提供这些分离物的各种用途，包括构建包含猿猴腺病毒41序列和在调控序列控制下的异源基因的重组载体。 还公开了表达猿腺病毒41基因的细胞系。 提供了使用载体和细胞系的方法。
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16.发明申请SIMIAN ADENOVIRUSES SAdV-43, -45, -46, -47, -48, -49, AND -50 AND USES THEREOF 有权SIMIAN ADENOVIRUSES SAdV-43，-45，-46，-47，-48，-49，AND-50及其用途公开(公告)号：US20110223135A1
公开(公告)日：2011-09-15
申请号：US13126557
申请日：2009-10-29
CPC分类号： C12N15/86 , C12N2710/10321 , C12N2710/10322 , C12N2710/10341 , C12N2710/10342 , C12N2710/10343 , C12N2710/10345 , C12N2710/10352 , C12N2810/6018
摘要： A recombinant vector comprises simian adenovirus 43, 45, 46, 47, 48, 49 or 50 sequences and a heterologous gene under the control of regulatory sequences. A cell line which expresses simian adenovirus 43, 45, 46, 47, 48, 49 or 50 gene(s) is also disclosed. Methods of using the vectors and cell lines are provided.
摘要翻译： 重组载体包含猿猴腺病毒43,45,46,47,48,49或50个序列，以及在调控序列控制下的异源基因。 还公开了表达猿腺病毒43,45,46,47,48,49或50个基因的细胞系。 提供了使用载体和细胞系的方法。
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17.发明申请SIMIAN ADENOVIRUSES SADV-36, -42.1, -42.2, AND -44 AND USES THEREOF 有权SIMIAN ADENOVIRUSES SADV-36，-42.1，-42.2和-44及其用途公开(公告)号：US20110008295A1
公开(公告)日：2011-01-13
申请号：US12920648
申请日：2009-03-03
IPC分类号： A61K35/76 , C12N7/00 , C12N7/01 , C07H21/00 , C12N15/63 , C07K14/075 , A61K38/00 , A61P35/00 , A61P37/00
CPC分类号： C12N7/00 , A61K35/761 , A61K39/145 , A61K2039/5256 , A61K2039/57 , C07K14/005 , C12N15/86 , C12N2710/10321 , C12N2710/10322 , C12N2710/10343 , C12N2710/10371 , C12N2760/16122 , C12N2760/16134 , C12N2760/16171
摘要： A recombinant vector comprises simian adenovirus 36, simian adenovirus 42.1, simian adenovirus 42.2 and/or simian adenovirus 44 sequences and a heterologous gene under the control of regulatory sequences. A cell line which expresses one or more simian adenovirus-36, -42.1, -42.2 or -44 gene(s) is also described. Methods of using the vectors and cell lines are provided.
摘要翻译： 重组载体包含猿猴腺病毒36，猿猴腺病毒42.1，猿腺病毒42.2和/或猿腺病毒44序列和在调节序列控制下的异源基因。 还描述了表达一种或多种猿猴腺病毒-36，-42.1，-42.2或-44基因的细胞系。 提供了使用载体和细胞系的方法。
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18.发明申请SIMIAN SUBFAMILY B ADENOVIRUSES SAdV-28, -27, -29, -32, -33, AND -35 AND USES THEREOF 有权SIMIAN SUBFAMILY B腺病毒SAdV-28，-27，-29，-32，-33，AND -35及其用途公开(公告)号：US20100254947A1
公开(公告)日：2010-10-07
申请号：US12744375
申请日：2008-11-24
CPC分类号： A61K35/761 , A61K38/162 , C07K14/005 , C12N7/00 , C12N15/86 , C12N2710/10321 , C12N2710/10322 , C12N2710/10332 , C12N2710/10343 , C12N2710/10351 , C12N2810/6018 , Y02A50/463
摘要： A recombinant vector comprises simian adenovirus 28, simian adenovirus 27, simian adenovirus 32, simian adenovirus 33, and/or simian adenovirus 35 sequences and a heterologous gene under the control of regulatory sequences. A cell line which expresses one or more simian adenovirus-28, -27, -32, -33, or -35 genes is also disclosed. Methods of using the vectors and cell lines are provided.
摘要翻译： 重组载体包含猿猴腺病毒28，猿猴腺病毒27，猿猴腺病毒32，猿腺病毒33和/或猿腺病毒35序列和在调节序列控制下的异源基因。 还公开了表达一种或多种猿猴腺病毒-28,27，-32，-33或-35基因的细胞系。 提供了使用载体和细胞系的方法。
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公开(公告)号：US20090275107A1
公开(公告)日：2009-11-05
申请号：US12226588
申请日：2007-04-27
CPC分类号： C12N7/00 , A61K48/0091 , C07K14/005 , C12N2750/14151
摘要： A method for producing AAV, without requiring cell lysis, is described. The method involves harvesting AAV from the supernatant. For AAV having capsids with a heparin binding site, the method involves modifying the AAV capsids and/or the culture conditions to ablate the binding between the AAV heparin binding site and the cells, thereby allowing the AAV to pass into the supernatant, i.e., media. Thus, the method of the invention provides supernatant containing high yields of AAV which have a higher degree of purity from cell membranes and intracellular materials, as compared to AAV produced using methods using a cell lysis step.
摘要翻译： 描述了不需要细胞裂解的生产AAV的方法。 该方法包括从上清液中收获AAV。 对于具有肝素结合位点的衣壳的AAV，该方法包括修饰AAV衣壳和/或培养条件以消除AAV肝素结合位点与细胞之间的结合，从而允许AAV进入上清液，即培养基 。 因此，与使用细胞裂解步骤的方法产生的AAV相比，本发明的方法提供了含有高产率的AAV的上清液，其具有比细胞膜和细胞内物质更高的纯度。
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