公开(公告)号：US5510262A

公开(公告)日：1996-04-23

申请号：US153204

申请日：1993-11-16

Applicant: Gregory Stephanopoulos ,  Rahul Singhvi ,  Seujeung Park ,  Maria Flytzani-Stephanopoulos ,  Mark A. Applegate

Inventor： Gregory Stephanopoulos ,  Rahul Singhvi ,  Seujeung Park ,  Maria Flytzani-Stephanopoulos ,  Mark A. Applegate

IPC: C12M1/04 ,  C12M1/40 ,  C12M3/06 ,  C12N5/00 ,  C12M3/00

CPC classification number: C12M25/18 ,  C12M23/24 ,  C12M29/04 ,  C12M29/06 ,  C12N5/0068

Abstract: An apparatus and method are disclosed for culturing cells by flowing a medium through a packed bed of biocompatible macroporous ceramic particles disposed in a cell-culture reactor. In one embodiment, oxygen and other nutrients in the medium are transported significantly by convective flow through pores of the biocompatible macroporous ceramic particles to cells disposed within the pores. Oxygen can be introduced to the cell-culture reactor by sparging oxygen gas into the cell-culture reactor. Oxygen content in the medium can also be increased by increasing the solubility of oxygen in the medium, such as by adding perfluorocarbon or other oxygen carriers to the medium. The oxygen capacity of the reactor can be further increased by sparging oxygen-containing gas into the medium at a plurality of points in the packed bed, or by introducing oxygen gas to the medium through a solid-phase oxygen gas-permeable membrane disposed in the packed bed. Alternatively, an oxygen-containing gas can be directed through an oxygen-permeable conduit disposed within at least one passage of a biocompatible macroporous support.

Abstract translation: 公开了一种用于通过使介质流过细胞培养反应器中的生物相容性大孔陶瓷颗粒的填充床来培养细胞的装置和方法。 在一个实施方案中，培养基中的氧和其他营养物质通过生物相容性大孔陶瓷颗粒的孔对流流动被显着转运到设置在孔内的细胞中。 可以通过将氧气喷射到细胞培养反应器中来将氧气引入细胞培养反应器。 培养基中的氧含量也可以通过增加介质中氧的溶解度来增加，例如通过向培养基中加入全氟化碳或其它氧载体。 通过在填充床中的多个点处将含氧气体喷射到介质中，或者通过将氧气引入到介质中，通过设置在所述填充床中的固相氧气可渗透膜，可以进一步增加反应器的氧气容量 包装床 或者，可以将含氧气体引导通过设置在生物相容性大孔载体的至少一个通道内的透氧导管。