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    Apparatus for the large scale growth and packaging of cell suspensions and three-dimensional tissue cultures
    3.
    发明授权
    Apparatus for the large scale growth and packaging of cell suspensions and three-dimensional tissue cultures 失效
    用于大规模生长和包装细胞悬浮液和三维组织培养物的装置

    公开(公告)号：US5763267A

    公开(公告)日：1998-06-09

    申请号：US632972

    申请日：1996-04-16

    Applicant: Christine Kurjan ,  Mark A. Applegate ,  James H. Flatt ,  Dawn Orton Applegate ,  Nicole Bloom ,  Mark Baumgartner

    Inventor： Christine Kurjan ,  Mark A. Applegate ,  James H. Flatt ,  Dawn Orton Applegate ,  Nicole Bloom ,  Mark Baumgartner

    IPC: C12N1/32 ,  C12C1/00 ,  C12M1/32 ,  C12M3/00 ,  C12N1/22 ,  C12N5/00 ,  C12N5/06 ,  C12M1/22

    CPC classification number: C12M23/26 ,  C12M23/34 ,  C12M23/40 ,  C12M45/22

    Abstract: An apparatus for the large scale culturing and packaging of cell suspensions, three dimensional tissue, and other biological systems is disclosed. The apparatus includes a plurality of flexible or semi-flexible treatment chambers comprising one or more individual culture pockets, a plurality of rigid spacers, an inlet fluid manifold, an outlet fluid manifold, a fluid reservoir, and a means for transporting fluid within the system. During treatment, liquid media is transported from the fluid reservoir to the inlet manifold, which will in turn evenly distribute the media to each of the connected treatment chambers and internal culture pockets. An outlet fluid manifold is also provided to ensure that each treatment chamber is evenly filled and to ensure that any air bubbles formed during treatment are removed from the treatment chambers. The treatment chambers are flexible or semi-flexible so as to provide for easy end-user handling during rinsing and application of the cultured transplants. Due to the flexibility of the treatment chambers, rigid spacers are also provided which ensure even fluid distribution within the chambers during treatment.

    Abstract translation: 公开了用于大规模培养和包装细胞悬浮液，三维组织和其他生物系统的装置。 该装置包括多个柔性或半柔性治疗室，其包括一个或多个单个培养袋，多个刚性隔离物，入口流体歧管，出口流体歧管，流体储存器和用于在系统内输送流体的装置 。 在处理期间，液体介质从流体储存器输送到入口歧管，这将进而将介质均匀地分配到每个连接的处理室和内部培养袋中。 还提供出口流体歧管以确保每个处理室均匀地填充并且确保处理期间形成的任何气泡从处理室中移除。 处理室是柔性的或半柔性的，以便在冲洗和应用培养移植物期间提供容易的最终使用者处理。 由于处理室的灵活性，还提供了刚性间隔件，其确保处理期间室内均匀的流体分布。

    Cell-culturing apparatus and method employing a macroporous support
    4.
    发明授权
    Cell-culturing apparatus and method employing a macroporous support 失效
    细胞培养装置和使用大孔支持物的方法

    公开(公告)号：US5262320A

    公开(公告)日：1993-11-16

    申请号：US921920

    申请日：1992-07-29

    Applicant: Gregory Stephanopoulos ,  Rahul Singhvi ,  Seujeung Park ,  Maria Flytzani-Stephanopoulos ,  Mark A. Applegate

    Inventor： Gregory Stephanopoulos ,  Rahul Singhvi ,  Seujeung Park ,  Maria Flytzani-Stephanopoulos ,  Mark A. Applegate

    IPC: C12M1/04 ,  C12M1/40 ,  C12M3/06 ,  C12N5/00 ,  A01N1/02 ,  C12M3/00

    CPC classification number: C12M25/18 ,  C12M23/24 ,  C12M29/04 ,  C12M29/06 ,  C12N5/0068

    Abstract: An apparatus and method are disclosed for culturing cells by flowing a medium through a packed bed of biocompatible macroporous ceramic particles disposed in a cell-culture reactor. In one embodiment, oxygen and other nutrients in the medium are transported significantly by convective flow through pores of the biocompatible macroporous ceramic particles to cells disposed within the pores. Oxygen can be introduced to the cell-culture reactor by sparging oxygen gas into the cell-culture reactor. Oxygen content in the medium can also be increased by increasing the solubility of oxygen in the medium, such as by adding perfluorocarbon or other oxygen carriers to the medium. The oxygen capacity of the reactor can be further increased by sparging oxygen-containing gas into the medium at a plurality of points in the packed bed, or by introducing oxygen gas to the medium through a solid-phase oxygen gas-permeable membrane disposed in the packed bed. Alternatively, an oxygen-containing gas can be directed through an oxygen-permeable conduit disposed within at least one passage of a biocompatible macroporous support.

    Abstract translation: 公开了一种用于通过使介质流过细胞培养反应器中的生物相容性大孔陶瓷颗粒的填充床来培养细胞的装置和方法。 在一个实施方案中，培养基中的氧和其他营养物质通过生物相容性大孔陶瓷颗粒的孔对流流动被显着转运到设置在孔内的细胞中。 可以通过将氧气喷射到细胞培养反应器中来将氧气引入细胞培养反应器。 培养基中的氧含量也可以通过增加介质中氧的溶解度来增加，例如通过向培养基中加入全氟化碳或其它氧载体。 通过在填充床中的多个点处将含氧气体喷射到介质中，或者通过将氧气引入到介质中，通过设置在所述填充床中的固相氧气可渗透膜，可以进一步增加反应器的氧气容量 包装床 或者，可以将含氧气体引导通过设置在生物相容性大孔载体的至少一个通道内的透氧导管。

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