公开(公告)号：US07951572B2

公开(公告)日：2011-05-31

申请号：US11711036

申请日：2007-02-27

Applicant: Hak-Sung Kim ,  Young-Pil Kim ,  Eunkeu Oh ,  Mi-Young Hong ,  Dohoon Lee ,  Tae Geol Lee ,  Dae Won Moon

Inventor： Hak-Sung Kim ,  Young-Pil Kim ,  Eunkeu Oh ,  Mi-Young Hong ,  Dohoon Lee ,  Tae Geol Lee ,  Dae Won Moon

IPC: C12N9/50 ,  C07K5/00

CPC classification number: G01N33/6848 ,  B82Y15/00 ,  C12Q1/00 ,  C12Q1/37 ,  C12Q1/42 ,  C12Q1/485 ,  G01N33/553 ,  G01N33/588 ,  G01N2500/04 ,  G01N2610/00

Abstract: Disclosed herein is a gold nanoparticle (AuNP)-based peptide chip prepared by forming a monolayer of AuNPs onto a self-assembled monolayer constructed on a solid support, and then immobilizing a peptide on the AuNPs. The AuNPs can effectively amplify the mass signal of the peptide, thus making it possible to measure the mass change of the peptide in a simple and accurate manner. Also, when secondary ion mass spectrometric analysis (spectrum or imaging) is performed on the AuNP-based peptide chip, the activities of enzymes and related inhibitors can be effectively quantified. The disclosed invention enables various enzyme activities to be analyzed rapidly and accurately, and thus can provide an important method for disease diagnosis and new drug development through the elucidation of signaling and interaction mechanisms.

Abstract translation: 本文公开了通过在固体支持物上构建的自组装单层上形成单层AuNP，然后将肽固定在AuNP上而制备的金纳米颗粒（AuNP）基肽芯片。 AuNPs可以有效地扩增肽的质量信号，从而使得可以以简单和准确的方式测量肽的质量变化。 此外，当在基于AuNP的肽芯片上进行二次离子质谱分析（光谱或成像）时，可以有效地量化酶和相关抑制剂的活性。 所公开的发明使得能够快速且准确地分析各种酶活性，因此可以通过阐明信号和相互作用机制来提供疾病诊断和新药开发的重要方法。

公开(公告)号：US20130173170A1

公开(公告)日：2013-07-04

申请号：US13405147

申请日：2012-02-24

Applicant: Tae Geol LEE ,  Dae Hee Hwang ,  So Jeong Yun ,  Ji Won Park

Inventor： Tae Geol LEE ,  Dae Hee Hwang ,  So Jeong Yun ,  Ji Won Park

IPC: G06F19/10

CPC classification number: G16C20/90 ,  G16C20/20 ,  G16C20/70

Abstract: Disclosed is a web-based platform for analyzing large-scale TOF-SIMS data and a method thereof. The web-based platform for analyzing large-scale TOF-SIMS data according to the present invention comprises: a communication unit for providing a connected user terminal with a web page and receiving a file for analyzing the TOF-SIMS data from the user terminal via the provided web page; a processing unit for analyzing the TOF-SIMS data using the received file and providing the user terminal with information created based on a result of the analysis via the communication unit; and a storage unit for storing the information created based on the result of the analysis. Through the platform, the present invention may provide a web-based tool that can be used in an automated analysis method.

Abstract translation: 公开了用于分析大规模TOF-SIMS数据的基于网络的平台及其方法。 用于分析根据本发明的大规模TOF-SIMS数据的基于web的平台包括：通信单元，用于向连接的用户终端提供网页，并且从用户终端接收用于从用户终端分析TOF-SIMS数据的文件， 提供的网页; 处理单元，用于使用接收到的文件分析TOF-SIMS数据，并且经由通信单元向用户终端提供基于分析结果创建的信息; 以及存储单元，用于存储基于分析结果创建的信息。 通过该平台，本发明可提供可用于自动分析方法中的基于网络的工具。

公开(公告)号：US08283117B2

公开(公告)日：2012-10-09

申请号：US12994263

申请日：2008-06-25

Applicant: Tae Geol Lee ,  Dae Won Moon ,  Byong Chul Yoo ,  In Hoo Kim

Inventor： Tae Geol Lee ,  Dae Won Moon ,  Byong Chul Yoo ,  In Hoo Kim

IPC: A61K38/21

CPC classification number: G01N33/6851 ,  G01N33/57446 ,  Y10T436/173845 ,  Y10T436/200833

Abstract: The present invention relates to a disease diagnosis method, a marker screening method, and a marker using a time-of-flight secondary ion mass spectrometry (TOF-SIMS), and more particularly, to a large intestine cancer diagnosis method, a large intestine cancer marker screening method, and a large intestine cancer marker using a time-of-flight secondary ion mass spectrometry (TOF-SIMS). Specifically, the present invention provides a method diagnosing a disease using a pattern of secondary ion mass (m/z) peaks of biological samples measured using a time-of- flight secondary ion mass spectrometry (TOF-SIMS) as a marker, a marker screening method being a reference judging an existence or non-existence of a disease, and a marker configured of specific secondary ion mass peaks.

Abstract translation: 本发明涉及疾病诊断方法，标记筛选方法和使用飞行时间二次离子质谱法（TOF-SIMS）的标记物，更具体地，涉及大肠癌诊断方法，大肠 癌症标志物筛选方法和使用飞行时间二次离子质谱（TOF-SIMS）的大肠癌标记物。 具体地，本发明提供了使用以飞行时间二次离子质谱法（TOF-SIMS）作为标记测定的生物样品的二次离子质量（m / z）峰的图案，标记物，标记 筛选方法是判断疾病的存在或不存在的参考，以及由特定二次离子质量峰构成的标记。