公开(公告)号：US06624145B1

公开(公告)日：2003-09-23

申请号：US09547621

申请日：2000-04-12

Applicant: Kenneth E. Narva ,  H. Ernest Schnepf ,  Mark Knuth ,  Michael R. Pollard ,  Guy A. Cardineau ,  George E. Schwab ,  Tracy Ellis Michaels

Inventor： Kenneth E. Narva ,  H. Ernest Schnepf ,  Mark Knuth ,  Michael R. Pollard ,  Guy A. Cardineau ,  George E. Schwab ,  Tracy Ellis Michaels

IPC: A01N3718

CPC classification number: C07K14/325 ,  C12N2799/026

Abstract: The subject invention concerns new classes of pesticidal toxins and the polynucleotide sequences which encoded these toxins. Also described are novel pesticidal isolates of Bacillus thuringiensis.

公开(公告)号：US6071511A

公开(公告)日：2000-06-06

申请号：US100437

申请日：1998-06-19

Applicant: Jewel M. Payne ,  Tracy Ellis Michaels ,  Gregory A. Bradfisch ,  Judy Muller-Cohn ,  Jenny Fu

Inventor： Jewel M. Payne ,  Tracy Ellis Michaels ,  Gregory A. Bradfisch ,  Judy Muller-Cohn ,  Jenny Fu

IPC: A01N63/00 ,  C12N5/04 ,  C12N5/10

CPC classification number: A01N63/00

Abstract: The subject invention concerns Bacillus thuringiensis microbes with activity against select coleopteran pests, e.g., Diabrotica sp., Hypera sp., and various flea beetles. For example, the B.t. isolates of the invention are active against alfalfa weevils (AW, Hypera brunneipennis), rape flea beetles (RFB, Phyllotreta cruciferae), and corn rootworms (CRW, Diabrotica undecimpunctata undecimpunctata). Thus, these microbes can be used to control these pests. Further, genes encoding toxins active against these pests can be isolated from the B.t. isolates and used to transform other microbes. The transformed microbes then can be used to control susceptible coleopteran pests. In preferred embodiments, a gene from PS140E2 is used to transform plants so that the transformed plants are resistant to flea beetles of the genus Phyllotreta.

Abstract translation: 本发明涉及具有针对选择性鞘翅目害虫的活性的苏云金芽孢杆菌微生物，例如Diabrotica sp。，Hypera sp。和各种跳蚤甲虫。 例如，B.t. 本发明的分离株对苜蓿象鼻虫（AW，Hypera brunneipennis），强奸跳蚤（RFB，十字花科植物）和玉米根虫（CRW，Diabrotica undecimpunctata undecimpunctata）具有活性。 因此，这些微生物可用于控制这些害虫。 此外，编码对这些害虫有活性的毒素的基因可以从B.t. 分离并用于转化其他微生物。 然后转化的微生物可用于控制易感的鞘翅目害虫。 在优选的实施方案中，使用来自PS140E2的基因来转化植物，使得转化的植物对Phyllotreta属的跳蚤甲虫具有抗性。

公开(公告)号：US07355003B2

公开(公告)日：2008-04-08

申请号：US11037780

申请日：2005-01-18

Applicant: Kenneth E. Narva ,  H. Ernest Schnepf ,  Mark Knuth ,  Michael R. Pollard ,  Guy A. Cardineau ,  George E. Schwab ,  Tracy Ellis Michaels

Inventor： Kenneth E. Narva ,  H. Ernest Schnepf ,  Mark Knuth ,  Michael R. Pollard ,  Guy A. Cardineau ,  George E. Schwab ,  Tracy Ellis Michaels

IPC: C07K14/325 ,  A01N63/02 ,  C12N15/32

CPC classification number: C12R1/075 ,  C07K14/325

Abstract: The subject invention concerns new classes of pesticidally active proteins and the polynucleotide sequences which encode these proteins. More specifically, in preferred embodiments, pesticidal proteins of approximately 40-50 kDa and of approximately 10-15 kDa are used for controlling corn rootworms. Also described are novel pesticidal isolates of Bacillus thuringiensis.

Abstract translation: 本发明涉及新类型的杀虫活性蛋白质和编码这些蛋白质的多核苷酸序列。 更具体地，在优选的实施方案中，使用约40-50kDa和约10-15kDa的杀虫蛋白来控制玉米根虫。 还描述了苏云金芽孢杆菌的新型杀虫分离物。

公开(公告)号：US06900371B2

公开(公告)日：2005-05-31

申请号：US10099278

申请日：2002-03-15

Applicant: Kenneth E. Narva ,  H. Ernest Schnepf ,  Mark Knuth ,  Michael R. Pollard ,  Guy A. Cardineau ,  George E. Schwab ,  Tracy Ellis Michaels ,  Stacey Finstad Lee ,  Paula Burmeister ,  Joanna Dojillo

Inventor： Kenneth E. Narva ,  H. Ernest Schnepf ,  Mark Knuth ,  Michael R. Pollard ,  Guy A. Cardineau ,  George E. Schwab ,  Tracy Ellis Michaels ,  Stacey Finstad Lee ,  Paula Burmeister ,  Joanna Dojillo

IPC: C12N15/09 ,  A01H1/00 ,  A01H5/00 ,  A01H5/10 ,  A01N37/18 ,  A01N63/00 ,  A01N63/02 ,  C07H21/04 ,  C07K14/325 ,  C12N1/15 ,  C12N1/19 ,  C12N1/20 ,  C12N1/21 ,  C12N5/04 ,  C12N5/10 ,  C12N9/00 ,  C12N15/32 ,  C12N15/62 ,  C12N15/82 ,  C12P21/02 ,  C12R1/07 ,  C12R1/39

CPC classification number: C12R1/075 ,  C07K14/325

Abstract: The subject invention concerns new classes of pesticidally active proteins and the polynucleotide sequences which encode these proteins. More specifically, in preferred embodiments, pesticidal proteins of approximately 40-50 kDa and of approximately 10-15 kDa are used for controlling corn rootworms. Also described are novel pesticidal isolates of Bacillus thuringiensis.

公开(公告)号：US06372480B1

公开(公告)日：2002-04-16

申请号：US09378088

申请日：1999-08-20

Applicant: Kenneth E. Narva ,  H. Ernest Schnepf ,  Mark Knuth ,  Michael R. Pollard ,  Guy A. Cardineau ,  George E. Schwab ,  Tracy Ellis Michaels ,  Stacey Finstad Lee ,  Paula Burmeister ,  Joanna Dojillo

Inventor： Kenneth E. Narva ,  H. Ernest Schnepf ,  Mark Knuth ,  Michael R. Pollard ,  Guy A. Cardineau ,  George E. Schwab ,  Tracy Ellis Michaels ,  Stacey Finstad Lee ,  Paula Burmeister ,  Joanna Dojillo

IPC: C07K14325

CPC classification number: C12R1/075 ,  C07K14/325

Abstract: The subject invention concerns new classes of pesticidally active proteins and the polynucleotide sequences which encode these proteins. More specifically, in preferred embodiments, pesticidal proteins of approximately 40-50 kDa and of approximately 10-15 kDa are used for controlling corn rootworms. Also described are novel pesticidal isolates of Bacillus thuringiensis.

Abstract translation: 本发明涉及新类型的杀虫活性蛋白质和编码这些蛋白质的多核苷酸序列。 更具体地，在优选的实施方案中，使用约40-50kDa和约10-15kDa的杀虫蛋白来控制玉米根虫。 还描述了苏云金芽孢杆菌的新型杀虫分离物。

公开(公告)号：US6150165A

公开(公告)日：2000-11-21

申请号：US224025

申请日：1998-12-31

Applicant: Jewel Payne ,  Kenneth E. Narva ,  Kendrick Akira Uyeda ,  Christine Julie Stalder ,  Tracy Ellis Michaels

Inventor： Jewel Payne ,  Kenneth E. Narva ,  Kendrick Akira Uyeda ,  Christine Julie Stalder ,  Tracy Ellis Michaels

IPC: C12N15/09 ,  A01N63/00 ,  A61K38/00 ,  C07K14/325 ,  C12N15/32 ,  C12P21/02 ,  C12R1/07 ,  C12R1/19 ,  C12N5/04 ,  C07H21/04 ,  C12N1/20 ,  C12N15/00

CPC classification number: A01N63/00 ,  C07K14/325 ,  C12R1/075 ,  A61K38/00

Abstract: Disclosed and claimed are approximately 130 kDa toxins produced by Bacillus thuringiensis PS201T6, which can be used to control pests. Further, claimed are novel genes encoding these .delta.-endotoxins, which can be expressed in other hosts. Expression of the .delta.-endotoxins in such hosts results in the control of susceptible insect pests in the environment of such hosts results in the control of susceptible insect pests in the environment of such hosts.

Abstract translation: 公开并要求的是由苏云金芽孢杆菌PS201T6产生的大约130kDa的毒素，其可以用于防治害虫。 此外，要求的是编码这些δ-内毒素的新基因，其可以在其他宿主中表达。 这种宿主中δ-内毒素的表达导致对这种宿主环境中的易感昆虫害虫的控制导致对这种宿主环境中的易感虫害的控制。