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公开(公告)号：US06369298B1
公开(公告)日：2002-04-09
申请号：US09056418
申请日：1998-04-07
Applicant: Tishu Cai , Dorothy A. Pierce , Laura A. Tagliani , Zuo-Yu Zhao
Inventor： Tishu Cai , Dorothy A. Pierce , Laura A. Tagliani , Zuo-Yu Zhao
IPC: A01H100
CPC classification number: C12N15/8205
Abstract: Methods and compositions for the efficient transformation of sorghum is provided. The method involves infection with Agrobacterium, particularly those comprising a super-binary vector. In this manner, any gene of interest can be introduced into the sorghum plant. The transformed gene will be flanked by at least one T-DNA border and present in the transformed sorghum in low copy number. Transformed sorghum, cells, tissues, plants, and seed are also provided. The invention encompasses regenerated, fertile sorghum plants, transgenic seeds produced therefrom, T1 and subsequent generations.
Abstract translation: 提供了高效转化高粱的方法和组成。 该方法涉及用农杆菌感染，特别是包含超二元载体的那些。 以这种方式，可以将任何感兴趣的基因引入高粱植物中。 转化的基因将侧接至少一个T-DNA边界并以低拷贝数存在于转化的高粱中。 还提供了转化的高粱，细胞，组织，植物和种子。 本发明包括再生的，可育的高粱植物，由其产生的转基因种子，T1和后代。
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公开(公告)号：US06175058B1
公开(公告)日：2001-01-16
申请号：US09263128
申请日：1999-03-05
Applicant: Christopher L. Baszczynski , Benjamin A. Bowen , Bruce J. Drummond , William J. Gordon-Kamm , David J. Peterson , Gary A. Sandahl , Laura A. Tagliani , Zuo-Yu Zhao , Grace marie St. Clair
Inventor： Christopher L. Baszczynski , Benjamin A. Bowen , Bruce J. Drummond , William J. Gordon-Kamm , David J. Peterson , Gary A. Sandahl , Laura A. Tagliani , Zuo-Yu Zhao , Grace marie St. Clair
IPC: A01H100
CPC classification number: C12N9/00 , C12N15/8213 , C12N15/8289
Abstract: A nucleic acid sequence effectively expressing FLP recombinase in monocot plants, particularly in maize. Stable, transformed maize plants harboring a gene encoding FLP or harboring FRT nucleic acid sequences enable efficient site-directed recombination of nucleic acid sequences in a monocot's genome.
Abstract translation: 在单子叶植物中，特别是在玉米中有效表达FLP重组酶的核酸序列。 含有编码FLP或携带FRT核酸序列的基因的稳定的转化的玉米植物能够在单子叶植物的基因组中有效地进行位点定向重组核酸序列。
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公开(公告)号：US5929301A
公开(公告)日：1999-07-27
申请号：US972258
申请日：1997-11-18
Applicant: Christopher L. Baszcynski , Benjamin A. Bowen , Bruce J. Drummond , William J. Gordon-Kamm , David J. Peterson , Gary A. Sandahl , Laura A. Tagliani , Zuo-Yu Zhao
Inventor： Christopher L. Baszcynski , Benjamin A. Bowen , Bruce J. Drummond , William J. Gordon-Kamm , David J. Peterson , Gary A. Sandahl , Laura A. Tagliani , Zuo-Yu Zhao
CPC classification number: C12N9/00 , C12N15/8213 , C12N15/8289
Abstract: A nucleic acid sequence effectively expressing FLP recombinase in monocot plants, particularly in maize. Stable, transformed maize plants harboring a gene encoding FLP or harboring FRT nucleic acid sequences enable efficient site-directed recombination of nucleic acid sequences in a monocot's genome.
Abstract translation: 在单子叶植物中，特别是在玉米中有效表达FLP重组酶的核酸序列。 含有编码FLP或携带FRT核酸序列的基因的稳定的转化的玉米植物能够在单子叶植物的基因组中有效地进行位点定向重组核酸序列。
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14.发明授权公开(公告)号：US08389807B2
公开(公告)日：2013-03-05
申请号：US12649673
申请日：2009-12-30
Applicant: Zuo-Yu Zhao , Xinli E. Wu , Myoeng-Je Cho , Deping Xu
Inventor： Zuo-Yu Zhao , Xinli E. Wu , Myoeng-Je Cho , Deping Xu
CPC classification number: C12N15/8209 , A01H1/04 , C12N15/8201 , C12N15/8205
Abstract: A method for increasing efficiency of germplasm screening for transformability may include providing a plurality of lines of plant target tissue to be transformed, characterizing each of the lines to provide characterization data, the characterization data comprises DNA or nucleic acid delivery technique response data and tissue culture response data, eliminating one or more of the plurality of lines based on the characterization data without performing transformation of the plurality of lines, such that a subset of the plurality of lines remains, and performing transformation experiments on the subset of the plurality of lines. The method may also include selecting a DNA or nucleic acid delivery technique protocol and a tissue culture protocol prior to the characterization.
Abstract translation: 提高种质筛选效率的方法可包括提供要转化的多条植物靶组织，表征各条线以提供特征数据，表征数据包括DNA或核酸递送技术应答数据和组织培养 响应数据，基于所述表征数据消除所述多行中的一个或多个，而不执行所述多条线的变换，使得保留所述多条线的子集，并对所述多条线的所述子集执行变换实验。 该方法还可以包括在表征之前选择DNA或核酸递送技术方案和组织培养方案。
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公开(公告)号：US08030560B2
公开(公告)日：2011-10-04
申请号：US12509538
申请日：2009-07-27
Applicant: Zuo-Yu Zhao , Ning Wang
Inventor： Zuo-Yu Zhao , Ning Wang
CPC classification number: A01H5/10
Abstract: A novel double haploid maize line designated PHWVZ and seed, plants and plant parts thereof. Methods for producing a maize plant that comprise crossing double haploid maize line PHWVZ with another maize plant. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into PHWVZ through backcross conversion and/or transformation, and to the maize seed, plant and plant part produced thereby. Hybrid maize seed, plant or plant part produced by crossing the double haploid line PHWVZ or a trait conversion of PHWVZ with another maize line. Inbred maize lines derived from double haploid maize line PHWVZ, methods for producing other inbred maize lines derived from double haploid maize line PHWVZ and the inbred maize lines and their parts derived by the use of those method.
Abstract translation: 一种新型双倍体玉米品系，命名为PHWVZ及其种子，植物和植物部分。 用于生产玉米植物的方法，其包括用另一种玉米植物杂交双倍体单倍体玉米品系PHWVZ。 用于生产含有其遗传物质的玉米植物的方法通过回交转化和/或转化渗透到PHWVZ中的一种或多种性状，以及由此产生的玉米种子，植物和植物部分。 杂交玉米种子，植物或植物部分通过双重单倍体系PHWVZ或PHWVZ与另一个玉米品系的转化而产生。 来自双倍体单倍体玉米品系PHWVZ的近交玉米品系，由双倍体单倍体玉米品系PHWVZ和近交玉米品系及其部分产生的其他近交玉米品系的生产方法。
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Patent Agency Ranking
16.发明申请公开(公告)号：US20110160087A1
公开(公告)日：2011-06-30
申请号：US12649673
申请日：2009-12-30
Applicant: ZUO-YU ZHAO , XINLI E. WU , MYOENG-JE CHO , DEPING XU
Inventor： ZUO-YU ZHAO , XINLI E. WU , MYOENG-JE CHO , DEPING XU
IPC: C40B30/06
CPC classification number: C12N15/8209 , A01H1/04 , C12N15/8201 , C12N15/8205
Abstract: A method for increasing efficiency of germplasm screening for transformability may include providing a plurality of lines of plant target tissue to be transformed, characterizing each of the lines to provide characterization data, the characterization data comprises DNA or nucleic acid delivery technique response data and tissue culture response data, eliminating one or more of the plurality of lines based on the characterization data without performing transformation of the plurality of lines, such that a subset of the plurality of lines remains, and performing transformation experiments on the subset of the plurality of lines. The method may also include selecting a DNA or nucleic acid delivery technique protocol and a tissue culture protocol prior to the characterization.
Abstract translation: 提高种质筛选效率的方法可包括提供要转化的多条植物靶组织，表征各条线以提供特征数据，表征数据包括DNA或核酸递送技术应答数据和组织培养 响应数据，基于所述表征数据消除所述多行中的一个或多个，而不执行所述多条线的变换，使得保留所述多条线的子集，并对所述多条线的所述子集执行变换实验。 该方法还可以包括在表征之前选择DNA或核酸递送技术方案和组织培养方案。
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公开(公告)号：US20090300789A1
公开(公告)日：2009-12-03
申请号：US12534507
申请日：2009-08-03
Applicant: Ning Wang , Lynn L. Heetland , William E. Solawetz , Zuo-Yu Zhao
Inventor： Ning Wang , Lynn L. Heetland , William E. Solawetz , Zuo-Yu Zhao
CPC classification number: A01H5/10
Abstract: A novel inbred maize line designated PHWWE and seed, plants and plant parts thereof. Methods for producing a maize plant that comprise crossing inbred maize line PHWWE with another maize plant. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into PHWWE through backcross conversion and/or transformation, and to the maize seed, plant and plant part produced thereby. Hybrid maize seed, plant or plant part produced by crossing the inbred line PHWWE or a trait conversion of PHWWE with another maize line. Inbred maize lines derived from maize line PHWWE, methods for producing other inbred maize lines derived from inbred maize line PHWWE and the inbred maize lines and their parts derived by the use of those methods.
Abstract translation: 一种新型近交玉米品系被指定为PHWWE及其种子，植物和植物部分。 用于生产玉米植物的方法，其包括用另一玉米植物杂交近交玉米品系PHWWE。 用于生产含有其遗传物质的玉米植物的方法通过回交转化和/或转化渗透到PHWWE中的一种或多种性状，以及由此产生的玉米种子，植物和植物部分的方法。 杂交玉米种子，植物或植物部分通过交叉自交系PHWWE或PHWWE与另一种玉米品系的转化而产生。 衍生自玉米系PHWWE的近交玉米品系，由近交玉米系PHWWE生产其他近交玉米品系的方法以及通过使用这些方法得到的近交玉米品系及其成分。
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公开(公告)号：US20090293141A1
公开(公告)日：2009-11-26
申请号：US12534487
申请日：2009-08-03
Applicant: Ning Wang , Michael D. Miller , Lynn L. Heetland , William E. Solawetz , Zuo-Yu Zhao
Inventor： Ning Wang , Michael D. Miller , Lynn L. Heetland , William E. Solawetz , Zuo-Yu Zhao
CPC classification number: A01H5/10
Abstract: A novel double haploid maize line designated PHWWD and seed, plants and plant parts thereof. Methods for producing a maize plant that comprise crossing double haploid maize line PHWWD with another maize plant. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into PHWWD through backcross conversion and/or transformation, and to the maize seed, plant and plant part produced thereby. Hybrid maize seed, plant or plant part produced by crossing the double haploid line PHWWD or a trait conversion of PHWWD with another maize line. Inbred maize lines derived from double haploid maize line PHWWD, methods for producing other inbred maize lines derived from double haploid maize line PHWWD and the inbred maize lines and their parts derived by the use of those methods.
Abstract translation: 一种新颖的双倍体单倍体玉米系命名为PHWWD及其种子，植物和植物部分。 用于生产玉米植物的方法，其包括用另一种玉米植物杂交双倍体单倍体玉米品系PHWWD。 用于生产含有其遗传物质的玉米植物的方法通过回交转化和/或转化渗透到PHWWD中的一种或多种性状，以及由此生产的玉米种子，植物和植物部分。 杂交玉米种子，植物或植物部分通过双重单倍体系PHWWD或PHWWD与另一个玉米品系的转化而产生。 来自双倍体单倍体玉米品系PHWWD的近交玉米品系，由双倍体单倍体玉米品系PHWWD生产其他近交玉米品系的方法，以及通过使用这些方法得到的近交玉米品系及其成分。
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公开(公告)号：US20080216198A1
公开(公告)日：2008-09-04
申请号：US12054556
申请日：2008-03-25
Applicant: Zuo-Yu Zhao , Dennis L. Bidney , Evan D. Elsing , Michael D. Miller , Xinli E. Wu , William J. Gordon-Kamm
Inventor： Zuo-Yu Zhao , Dennis L. Bidney , Evan D. Elsing , Michael D. Miller , Xinli E. Wu , William J. Gordon-Kamm
CPC classification number: C12N15/8241 , A01H5/10 , C12N15/8201 , C12N15/829
Abstract: Methods for producing homozygous plants, seeds, and plant cells are provided. Also provided are methods of transformation.
Abstract translation: 提供了用于产生纯合植物，种子和植物细胞的方法。 还提供了转化的方法。
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公开(公告)号：US20080216191A1
公开(公告)日：2008-09-04
申请号：US11532921
申请日：2006-09-19
Applicant: Joanne E. Barton , Sheila E. Maddock , Xinli E. Wu , Zuo-Yu Zhao , Mark E. Williams , Tanveer Hussain , William J. Gordon-Kamm
Inventor： Joanne E. Barton , Sheila E. Maddock , Xinli E. Wu , Zuo-Yu Zhao , Mark E. Williams , Tanveer Hussain , William J. Gordon-Kamm
CPC classification number: A01H1/08
Abstract: Methods for producing doubled haploid corn (Zea mays) plants, seeds, and plant cells are provided.
Abstract translation: 提供了生产双倍单倍体玉米（Zea mays）植物，种子和植物细胞的方法。
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