公开(公告)号：US09499616B2

公开(公告)日：2016-11-22

申请号：US14923966

申请日：2015-10-27

Applicant: AbbVie Inc.

Inventor： Kartik Subramanian ,  Mayda Perez Thiele ,  Xiaobei Zeng ,  Chee Furng Wong ,  Zehra Kaymakcalan ,  Ying Jing ,  Christopher M. Chumsae

IPC: C12P21/08 ,  C07K16/24 ,  C12P21/00

CPC classification number: C07K16/241 ,  A61K2039/505 ,  C07K2317/14 ,  C07K2317/21 ,  C07K2317/56 ,  C07K2317/565 ,  C12P21/005

Abstract: The instant invention relates to modulated lysine variant species compositions comprising a protein, e.g., an antibody, or antigen-binding portion thereof, and methods, e.g., cell culture and/or protein purification methods, for producing such modulated lysine variant species compositions. Methods for using such compositions to treat a disorder, e.g., a disorder in which TNFα is detrimental, are also provided.

公开(公告)号：US09266949B2

公开(公告)日：2016-02-23

申请号：US14575691

申请日：2014-12-18

Applicant: AbbVie, Inc.

Inventor： Natarajan Ramasubramanyan ,  Lihua Yang ,  Matthew Omon Herigstad ,  Hong Yang ,  Kartik Subramanian ,  Xiaobei Zeng ,  Diane D. Dong ,  Wen Chung Lim ,  Kathreen A. Gifford ,  Zehra Kaymakcalan ,  Christopher Chumsae

IPC: C12P21/00 ,  C07K16/24 ,  C07K16/00 ,  A61K39/00

CPC classification number: C07K16/241 ,  A61K39/39591 ,  A61K47/183 ,  A61K47/26 ,  A61K2039/505 ,  C07K16/00 ,  C07K2317/10 ,  C07K2317/14 ,  C07K2317/21 ,  C07K2317/41 ,  C07K2317/76

Abstract: The instant invention relates to low acidic species (AR) compositions comprising a protein, e.g., an antibody, or antigen-binding portion thereof, and methods, e.g., cell culture and/or protein purification methods, for producing such low AR compositions. Methods for using such compositions to treat a disorder, e.g., a disorder in which TNFα is detrimental, are also provided.

公开(公告)号：US09200070B2

公开(公告)日：2015-12-01

申请号：US14714113

申请日：2015-05-15

Applicant: AbbVie, Inc.

Inventor： Natarajan Ramasubramanyan ,  Lihua Yang ,  Matthew Omon Herigstad ,  Hong Yang ,  Kartik Subramanian ,  Xiaobei Zeng ,  Diane D. Dong ,  Wen Chung Lim ,  Kathreen A. Gifford ,  Zehra Kaymakcalan ,  Christopher Chumsae

IPC: A61K39/00 ,  C07K16/24

CPC classification number: C07K16/241 ,  A61K39/39591 ,  A61K47/183 ,  A61K47/26 ,  A61K2039/505 ,  C07K16/00 ,  C07K2317/10 ,  C07K2317/14 ,  C07K2317/21 ,  C07K2317/41 ,  C07K2317/76

Abstract: The instant invention relates to low acidic species (AR) compositions comprising a protein, e.g., an antibody, or antigen-binding portion thereof, and methods, e.g., cell culture and/or protein purification methods, for producing such low AR compositions. Methods for using such compositions to treat a disorder, e.g., a disorder in which TNFα is detrimental, are also provided.

公开(公告)号：US09181337B2

公开(公告)日：2015-11-10

申请号：US14077988

申请日：2013-11-12

Applicant: AbbVie, Inc.

Inventor： Kartik Subramanian ,  Mayda Perez Thiele ,  Xiaobei Zeng ,  Chee Furng Wong ,  Zehra Kaymakcalan ,  Ying Jing ,  Christopher Chumsae

IPC: A61K39/395 ,  C07K16/24

CPC classification number: C07K16/241 ,  A61K2039/505 ,  C07K2317/14 ,  C07K2317/21 ,  C07K2317/56 ,  C07K2317/565 ,  C12P21/005

Abstract: The instant invention relates to modulated lysine variant species compositions comprising a protein, e.g., an antibody, or antigen-binding portion thereof, and methods, e.g., cell culture and/or protein purification methods, for producing such modulated lysine variant species compositions. Methods for using such compositions to treat a disorder, e.g., a disorder in which TNFα is detrimental, are also provided.

Abstract translation: 本发明涉及包含蛋白质例如抗体或其抗原结合部分的调节的赖氨酸变体种组合物，以及用于产生这种经调节的赖氨酸变体种组合物的方法，例如细胞培养和/或蛋白质纯化方法。 还提供了使用这种组合物治疗病症（例如其中TNFα有害的病症）的方法。

公开(公告)号：US08921526B2

公开(公告)日：2014-12-30

申请号：US14206296

申请日：2014-03-12

Applicant: AbbVie Inc.

Inventor： Chris M. Chumsae ,  Zehra Kaymakcalan

IPC: C07K16/00 ,  C07K16/18 ,  A61K39/395 ,  C07K16/24

CPC classification number: C07K16/241 ,  C07K2317/40 ,  C07K2317/565 ,  C07K2317/94 ,  Y10S514/863

Abstract: The present invention is directed to modified antibodies, including anti-TNFα antibodies, in which C-terminal amino acids of heavy chain sequences are modified from a native sequence of proline-glycine-lysine (“PGK”) to one that includes a proline positioned between the glycine and lysine, resulting in a C-terminal sequence of proline-glycine-proline-lycine (“PGPK”). The invention further provides methods of producing and using such antibodies.

Abstract translation: 本发明涉及包含抗TNFα抗体的修饰抗体，其中重链序列的C末端氨基酸从脯氨酸 - 甘氨酸 - 赖氨酸天然序列（“PGK”）修饰为包含位于脯氨酸 - 甘氨酸 - 赖氨酸 在甘氨酸和赖氨酸之间产生脯氨酸 - 甘氨酸 - 脯氨酸 - 脯氨酸（“PGPK”）的C-末端序列。 本发明还提供了生产和使用这些抗体的方法。