公开(公告)号：US08921526B2

公开(公告)日：2014-12-30

申请号：US14206296

申请日：2014-03-12

Applicant: AbbVie Inc.

Inventor： Chris M. Chumsae ,  Zehra Kaymakcalan

IPC: C07K16/00 ,  C07K16/18 ,  A61K39/395 ,  C07K16/24

CPC classification number: C07K16/241 ,  C07K2317/40 ,  C07K2317/565 ,  C07K2317/94 ,  Y10S514/863

Abstract: The present invention is directed to modified antibodies, including anti-TNFα antibodies, in which C-terminal amino acids of heavy chain sequences are modified from a native sequence of proline-glycine-lysine (“PGK”) to one that includes a proline positioned between the glycine and lysine, resulting in a C-terminal sequence of proline-glycine-proline-lycine (“PGPK”). The invention further provides methods of producing and using such antibodies.

Abstract translation: 本发明涉及包含抗TNFα抗体的修饰抗体，其中重链序列的C末端氨基酸从脯氨酸 - 甘氨酸 - 赖氨酸天然序列（“PGK”）修饰为包含位于脯氨酸 - 甘氨酸 - 赖氨酸 在甘氨酸和赖氨酸之间产生脯氨酸 - 甘氨酸 - 脯氨酸 - 脯氨酸（“PGPK”）的C-末端序列。 本发明还提供了生产和使用这些抗体的方法。

公开(公告)号：US09612247B2

公开(公告)日：2017-04-04

申请号：US14161552

申请日：2014-01-22

Applicant: AbbVie, Inc.

Inventor： Chris M. Chumsae ,  Czeslaw H. Radziejewskia ,  Anton Manuilov

IPC: G01N33/68 ,  C07K16/22 ,  C07K16/28 ,  C07K16/32 ,  C07K16/46 ,  C07K16/24 ,  C07K16/42

CPC classification number: G01N33/6854 ,  C07K16/22 ,  C07K16/244 ,  C07K16/32 ,  C07K16/4291 ,  C07K16/468 ,  C07K2317/94 ,  G01N33/6815

Abstract: Provided are methods for measuring the inherent stability of intrachain disulphide-containing domains (e.g., antibody variable domains) and for optimizing the positioning of intrachain disulphide-containing domains within a protein (e.g., a multispecific binding protein, e.g., a DVD-Ig). Also provided are methods of making multispecific binding proteins (e.g., DVD-Ig molecules) comprising two or more antibody variable domains in which the antibody variable domains are optimally positioned within the multispecific binding proteins to enhance stability of the multispecific binding protein. Multispecific binding proteins optimized using the methods disclosed herein are also provided.

公开(公告)号：US20160185848A1

公开(公告)日：2016-06-30

申请号：US14795838

申请日：2015-07-09

Applicant: ABBVIE INC.

Inventor： Patrick Hossler ,  Sean McDermott ,  Christopher Racicot ,  Joseph G. Matuck ,  Keith Cochran ,  Chris M. Chumsae

IPC: C07K16/24 ,  C12P21/00

CPC classification number: C07K16/241 ,  C07K16/00 ,  C07K2317/14 ,  C07K2317/24 ,  C07K2317/41 ,  C12N5/0037 ,  C12N2500/34 ,  C12N2510/02 ,  C12P21/005

Abstract: The present invention relates to the field of protein production, and in particular to methods and compositions for modulating glycosylation of recombinant proteins expressed in host cells.

Abstract translation: 本发明涉及蛋白质生产领域，特别涉及用于调节在宿主细胞中表达的重组蛋白质糖基化的方法和组合物。

公开(公告)号：US20150132320A1

公开(公告)日：2015-05-14

申请号：US14570489

申请日：2014-12-15

Applicant: AbbVie, Inc.

Inventor： Chris M. Chumsae ,  Zehra Kaymakcalan

IPC: C07K16/24

CPC classification number: C07K16/241 ,  C07K2317/40 ,  C07K2317/565 ,  C07K2317/94 ,  Y10S514/863

Abstract: The present invention is directed to modified antibodies, including anti-TNFα antibodies, in which C-terminal amino acids of heavy chain sequences are modified from a native sequence of proline-glycine-lysine (“PGK”) to one that includes a proline positioned between the glycine and lysine, resulting in a C-terminal sequence of proline-glycine-proline-lycine (“PGPK”). The invention further provides methods of producing and using such antibodies.

Abstract translation: 本发明涉及包含抗TNFα抗体的修饰抗体，其中重链序列的C末端氨基酸从脯氨酸 - 甘氨酸 - 赖氨酸天然序列（“PGK”）修饰为包含位于脯氨酸 - 甘氨酸 - 赖氨酸 在甘氨酸和赖氨酸之间产生脯氨酸 - 甘氨酸 - 脯氨酸 - 脯氨酸（“PGPK”）的C-末端序列。 本发明还提供了生产和使用这些抗体的方法。

公开(公告)号：US20140271626A1

公开(公告)日：2014-09-18

申请号：US14206296

申请日：2014-03-12

Applicant: AbbVie Inc.

Inventor： Chris M. Chumsae ,  Zehra Kaymakcalan

IPC: C07K16/24

CPC classification number: C07K16/241 ,  C07K2317/40 ,  C07K2317/565 ,  C07K2317/94 ,  Y10S514/863

Abstract: The present invention is directed to modified antibodies, including anti-TNFα antibodies, in which C-terminal amino acids of heavy chain sequences are modified from a native sequence of proline-glycine-lysine (“PGK”) to one that includes a proline positioned between the glycine and lysine, resulting in a C-terminal sequence of proline-glycine-proline-lycine (“PGPK”). The invention further provides methods of producing and using such antibodies.

Abstract translation: 本发明涉及包含抗TNFα抗体的修饰抗体，其中重链序列的C末端氨基酸从脯氨酸 - 甘氨酸 - 赖氨酸天然序列（“PGK”）修饰为包含位于脯氨酸 - 甘氨酸 - 赖氨酸 在甘氨酸和赖氨酸之间产生脯氨酸 - 甘氨酸 - 脯氨酸 - 脯氨酸（“PGPK”）的C-末端序列。 本发明还提供了生产和使用这些抗体的方法。