公开(公告)号：US20080081340A1

公开(公告)日：2008-04-03

申请号：US11529574

申请日：2006-09-29

申请人： Anil Patwardhan ,  Handong Li ,  Narayan Sundararajan

发明人： Anil Patwardhan ,  Handong Li ,  Narayan Sundararajan

IPC分类号： G01N33/53 ,  G01N33/553 ,  C12P21/06 ,  C12M3/00

CPC分类号： G01N33/68 ,  C12P21/06 ,  G01N33/54373

摘要： The sensitivity of surface enhanced Raman spectroscopy to silver nano-particle/peptide aggregates is increased by prior treatment of the peptides. According to a first type of embodiment, an enzyme such as Glu-C is used for protein(s) digestion based on the enzyme's ability to cleave proteins at a selected location having a negative charge, such as at aspartic acid and glutamic acid. This type of digestion is used to derive a higher proportion of positively charged component peptides sequences as compared to the component peptides sequences obtained by standard tryptic digestion of protein(s). According to a second type of embodiment, methyl-esterification of peptides suppresses the negative charge contributions of portions of the peptides such as aspartic acid, glutamic acid, and the C-terminus. Both types of embodiments result in increased binding affinity of the resulting component sequence peptides with negatively charged nano-particles such as silver nano-particles. According to yet other embodiments, the first and second types of embodiments can be combined for further sensitivity increase.

摘要翻译： 表面增强拉曼光谱对银纳米颗粒/肽聚集体的敏感性通过先前处理肽而增加。 根据第一类型的实施方案，基于酶在具有负电荷的选定位置（例如天冬氨酸和谷氨酸）切割蛋白质的能力，使用诸如Glu-C的酶进行蛋白质消化。 与通过蛋白质的标准胰蛋白酶消化获得的成分肽序列相比，这种类型的消化用于导出更高比例的带正电荷的组分肽序列。 根据第二类型的实施方案，肽的甲基酯化抑制了肽部分的负电荷贡献，例如天冬氨酸，谷氨酸和C末端。 两种类型的实施方案导致所得组分序列肽与带负电荷的纳米颗粒如银纳米颗粒的结合亲和力增加。 根据其他实施例，可以组合第一和第二类型的实施例以进一步提高灵敏度。

公开(公告)号：US20050119415A1

公开(公告)日：2005-06-02

申请号：US10982942

申请日：2004-11-05

申请人： John Dapron ,  William Kappel ,  Handong Li

发明人： John Dapron ,  William Kappel ,  Handong Li

IPC分类号： G01N33/48 ,  C12M1/00 ,  G01N33/543 ,  C08F2/00

CPC分类号： G01N33/54346 ,  G01N33/54366

摘要： A high capacity assay platform capable of binding target molecules includes a substrate and a polymer matrix attached to the substrate. The polymer matrix comprises a plurality of polymer molecules where at least some of the polymer molecules are covalently attached directly to the substrate and at least some of which molecules are crosslinked to other polymer molecules. Some of the polymer molecules have at least one binding ligand covalently attached thereto, and the density of the polymer matrix on the substrate is at least 2 μg/cm2.

摘要翻译： 能够结合靶分子的高容量测定平台包括底物和附着于基底的聚合物基质。 聚合物基质包括多个聚合物分子，其中至少一些聚合物分子直接共价连接到基底上，并且其中至少一些分子与其它聚合物分子交联。 一些聚合物分子具有共价连接到其上的至少一个结合配体，并且基材上的聚合物基质的密度为至少2mug / cm 2。

公开(公告)号：US20050003496A1

公开(公告)日：2005-01-06

申请号：US10745916

申请日：2003-12-23

申请人： Glenn McGall ,  Anthony Barone ,  Handong Li

发明人： Glenn McGall ,  Anthony Barone ,  Handong Li

IPC分类号： C12P17/00 ,  C07

CPC分类号： C12P17/00

摘要： Nucleic acid labeling compounds containing heterocyclic derivatives are disclosed. Methods for making such heterocyclic compounds are also disclosed. The labeling compounds are suitable for enzymatic attachment to a nucleic acid, either terminally or internally, to provide a mechanism of nucleic acid detection.

摘要翻译： 公开了含有杂环衍生物的核酸标记化合物。 还公开了制备这种杂环化合物的方法。 标记化合物适合于在末端或内部酶促附着于核酸，以提供核酸检测机制。

公开(公告)号：US20140178862A1

公开(公告)日：2014-06-26

申请号：US13721488

申请日：2012-12-20

申请人： Xing Su ,  Handong Li

发明人： Xing Su ,  Handong Li

IPC分类号： G01N27/00

CPC分类号： C12Q1/6874 ,  G01N27/3277 ,  C12Q2565/607

摘要： Photoinduced redox current (PIRC) detection for DNA sequencing using integrated transducer arrays is described. For example, a method of determining DNA sequencing using photoinduced redox current (PIRC) includes providing a template having a primer region and a second region of one or more unknown bases. The template is coupled to a transducer array. The method also includes incorporating a tagged nucleotide at the second region of the template. The method also includes exposing the template to a light source. The method also includes electrically detecting, by the transducer, a state of the template. The method also includes removing a photosensitizer tag of the tagged nucleotide.

摘要翻译： 描述了使用集成的换能器阵列的DNA测序的光诱导的氧化还原电流（PIRC）检测。 例如，使用光诱导氧化还原电流（PIRC）测定DNA测序的方法包括提供具有引物区和一个或多个未知碱基的第二区的模板。 模板耦合到换能器阵列。 该方法还包括在模板的第二区域掺入标记的核苷酸。 该方法还包括将模板暴露于光源。 该方法还包括通过换能器电检测模板的状态。 该方法还包括去除标记的核苷酸的光敏剂标签。

公开(公告)号：US20130264669A1

公开(公告)日：2013-10-10

申请号：US13442957

申请日：2012-04-10

申请人： Handong Li ,  Michael Prokesch ,  John Francis Eger

发明人： Handong Li ,  Michael Prokesch ,  John Francis Eger

IPC分类号： H01L27/14 ,  H01L31/18

CPC分类号： H01L31/022408 ,  H01L27/14676 ,  H01L27/14696 ,  H01L31/085 ,  H01L31/1828 ,  Y02E10/543

摘要： A method of making a semiconductor radiation detector wherein the metal layers which serve as the cathode and anode electrodes are recessed from the designated prospective dice lines which define the total upper and lower surface areas for each detector such that the dicing blade will not directly engage the metal during dicing and therefore prevent metal from intruding upon (smearing) the vertical side walls of the detector substrate.

摘要翻译： 一种制造半导体辐射检测器的方法，其中用作阴极和阳极电极的金属层从指定的预期骰子线凹下，其限定每个检测器的总上表面积和下表面积，使得切割刀片不会直接接合 金属，因此防止金属侵入（污染）检测器基板的垂直侧壁。

公开(公告)号：US08003408B2

公开(公告)日：2011-08-23

申请号：US11319747

申请日：2005-12-29

申请人： Jingwu Zhang ,  Narayan Sundararajan ,  Sarah M. Ngola ,  Handong Li

发明人： Jingwu Zhang ,  Narayan Sundararajan ,  Sarah M. Ngola ,  Handong Li

IPC分类号： G01N33/553 ,  G01N33/20 ,  G01N21/00 ,  A61K9/54

CPC分类号： G01N33/54373 ,  B82Y30/00 ,  G01N21/658 ,  G01N33/54346 ,  G01N2021/653 ,  G01N2021/655 ,  G01N2021/656

摘要： A SERS active particle having a metal-containing particle and a cationic coating on the metal-containing particle, wherein the SERS active particle carries a positive charge is disclosed. Also, a SERS active particle having a metal-containing particle and a non-metallic molecule, wherein the metal-containing particle is derivatized with the non-metallic molecule is disclosed. In addition, several methods of modifying the nanoparticles surfaces of a SERS active particle and of improving the interaction between the SERS active particle and an analyte are disclosed.

摘要翻译： 公开了一种SERS活性颗粒，其具有含金属颗粒和阳离子涂层在含金属颗粒上，其中SERS活性颗粒携带正电荷。 另外，公开了含有金属的粒子和非金属分子的SERS活性粒子，其中含金属的粒子被非金属分子衍生化。 另外，公开了改进SERS活性颗粒的纳米颗粒表面和改善SERS活性颗粒与分析物之间的相互作用的几种方法。

公开(公告)号：US07753987B2

公开(公告)日：2010-07-13

申请号：US12067835

申请日：2006-04-11

申请人： Xiaolong Du ,  Zhaoquan Zeng ,  Hongtao Yuan ,  Handong Li ,  Qikun Xue ,  Jinfeng Jia

发明人： Xiaolong Du ,  Zhaoquan Zeng ,  Hongtao Yuan ,  Handong Li ,  Qikun Xue ,  Jinfeng Jia

IPC分类号： C22B9/04 ,  C22B9/05 ,  C22B26/00 ,  C22B30/04

CPC分类号： C22B9/02 ,  C22B9/04 ,  C22B9/14 ,  C22B19/12 ,  C22B19/18 ,  F27B14/04 ,  F27B14/20 ,  Y02P10/234

摘要： The present invention concerns a high vacuum in-situ refining method for high-purity and superhigh-purity materials and the apparatus thereof, characterized in heating the upper part and lower part of crucible separately using double-heating-wires diffusion furnace under vacuum, thereby forming the temperature profile which is high at upper part and low at lower part of crucible, or in reverse during different stages; then heating the crucible in two steps to remove impurities with high saturation vapor pressure and low saturation vapor pressure respectively in efficiency; and obtaining high-purity materials eventually. The whole procedure is isolated from atmosphere, reducing contamination upon stuff remarkably. The present invention could provide products with high-quality and high production capacity, which are stable in performance, therefore is reliable and free from contamination. The present invention is appropriate for manufacture of high-purity and superhigh-purity materials, particularly for manufacture of high-activity high-purity materials.

摘要翻译： 本发明涉及一种用于高纯度和超高纯度材料的高真空原位精制方法及其装置，其特征在于使用双重加热丝扩散炉在真空下分别加热坩埚的上部和下部，由此 形成坩埚上部和下部较高的温度分布，或在不同阶段形成相反的温度分布; 然后分两步加热坩埚以分别以高饱和蒸气压和低饱和蒸气压去除杂质; 并最终获得高纯度材料。 整个过程与大气隔离，减少了污染物的显着。 本发明可以提供具有高质量和高生产能力的产品，性能稳定，因此可靠，无污染。 本发明适用于制造高纯度和超高纯度材料，特别是用于制造高活性高纯度材料。

公开(公告)号：US20080003576A1

公开(公告)日：2008-01-03

申请号：US11477675

申请日：2006-06-30

申请人： Jingwu Zhang ,  Handong Li ,  Narayan Sundararajan ,  Xing Su ,  Lei Sun

发明人： Jingwu Zhang ,  Handong Li ,  Narayan Sundararajan ,  Xing Su ,  Lei Sun

IPC分类号： C12Q1/68 ,  G01N33/555

CPC分类号： G01N33/585 ,  C12Q1/6816 ,  G01N21/658 ,  G01N33/54373 ,  C12Q2563/155 ,  C12Q2565/632

摘要： The embodiments of the invention are directed to a SERS cluster comprising a capture particle that is at least partially surrounded by analyte molecules, wherein both the capture particle and the analyte molecules surrounding the capture particle are at least partially surrounded by enhancer particles, wherein a majority of the analyte molecules are either sandwiched between capture and enhancer particles or located between junctions of the enhancer particles. The embodiments of the invention also relate to methods of manufacturing and detecting the SERS cluster. The embodiments of the invention also relate to a SERS active particle comprising a tag molecule comprising a Raman active compound and a probe or a linker having a specific biochemical binding capability and to a method for detecting of a target molecule using a SERS active particle having a tag molecule comprising a Raman active compound and a probe or a linker.

摘要翻译： 本发明的实施方案涉及包含被分析物分子至少部分包围的捕获颗粒的SERS簇，其中捕获颗粒和捕获颗粒周围的分析物分子至少部分地被增强子颗粒包围，其中多数 分析物分子被夹在捕获和增强剂颗粒之间或位于增强剂颗粒的结之间。 本发明的实施例还涉及制造和检测SERS簇的方法。 本发明的实施方案还涉及包含包含拉曼活性化合物的标签分子和具有特定生物化学结合能力的探针或接头的SERS活性颗粒以及使用具有下列化合物的SERS活性颗粒检测靶分子的方法： 标签分子，其包含拉曼活性化合物和探针或接头。

公开(公告)号：US07291463B2

公开(公告)日：2007-11-06

申请号：US10745916

申请日：2003-12-23

申请人： Glenn H. McGall ,  Anthony D. Barone ,  Handong Li

发明人： Glenn H. McGall ,  Anthony D. Barone ,  Handong Li

IPC分类号： C07H7/06 ,  C07H19/10 ,  C07H21/02 ,  C07H21/04

CPC分类号： C12P17/00

摘要： A method for converting a pseudoisocytidine base having the formula: to a pseudoisocytidine molecule having the formula: is disclosed. Such compounds are useful as nucleic acid labeling compounds.

摘要翻译： 公开了将具有下式的伪异胞苷碱转换成具有下式的假异胞苷分子的方法。 这些化合物可用作核酸标记化合物。

公开(公告)号：US20050118709A1

公开(公告)日：2005-06-02

申请号：US10983034

申请日：2004-11-05

申请人： John Dapron ,  William Kappel ,  Handong Li

发明人： John Dapron ,  William Kappel ,  Handong Li

IPC分类号： G01N33/48 ,  C12M1/00 ,  G01N33/543 ,  A61M36/14

CPC分类号： G01N33/54346 ,  G01N33/54366

摘要： A high capacity assay platform capable of binding target molecules includes a substrate and a polymer matrix attached to the substrate. The polymer matrix comprises a plurality of polymer molecules where at least some of the polymer molecules are covalently attached directly to the substrate and at least some of which molecules are crosslinked to other polymer molecules. Some of the polymer molecules have at least one binding ligand covalently attached thereto, and the density of the polymer matrix on the substrate is at least 2 μg/cm2.