公开(公告)号：US20240279377A1

公开(公告)日：2024-08-22

申请号：US18554059

申请日：2022-04-13

Applicant: BGI Shenzhen Co., Ltd.

Inventor： Hongduan Huang ,  Handong Li

IPC: C08F257/02

CPC classification number: C08F257/02

Abstract: Techniques are described for preparing monodisperse polymer beads, such as using monosized seed particles, where the process of growing, polymerizing, or swelling the beads during preparation occurs at low, ambient, or room temperature. A variety of schemes are disclosed for growing, polymerizing, or swelling that avoid thermal initiation-based swelling. For example, photo polymerization, metal-free atom transfer radical polymerization, and redox polymerization schemes are disclosed. Additional features may be implemented in the monodisperse polymer beads, such as controlling bead density, controlling porous character, and inclusion of various chemical functionalities, including protected functionalities that can be activated or de-protected after preparation of the monodisperse polymer beads, such as during subsequent use.

公开(公告)号：US20210040556A1

公开(公告)日：2021-02-11

申请号：US16987092

申请日：2020-08-06

Applicant: BGI Shenzhen Co., Ltd.

Inventor： Yan Chen ,  Handong Li

IPC: C12Q1/6874 ,  C07D401/12 ,  G01N21/76

Abstract: Acridinium ester-containing compounds are provided herein. Also provided herein are chemiluminescence-based one-color sequencing methods and methods of using the acridinium ester-containing compounds in chemiluminescence-based one-color sequencing.

公开(公告)号：US20180223358A1

公开(公告)日：2018-08-09

申请号：US15862566

申请日：2018-01-04

Applicant: Complete Genomics, Inc. ,  BGI Shenzhen

Inventor： Radoje Drmanac ,  Snezana Drmanac ,  Handong Li ,  Xun Xu ,  Matthew J. Callow ,  Leon Eckhardt ,  Naibo Yang ,  Quan Ding

IPC: C12Q1/6874 ,  C12Q1/6876

CPC classification number: C12Q1/6874 ,  A61K47/00 ,  C07K16/2851 ,  C07K16/44 ,  C07K2317/73 ,  C12Q1/6804 ,  C12Q1/6869 ,  C12Q1/6876 ,  C12Q2525/186

Abstract: The invention provides compositions and methods for sequencing nucleic acids and other applications. In sequencing by synthesis, unlabeled reversible terminators are incorporated by a polymerase in each cycle, then labeled after incorporation by binding to the reversible terminator a directly or indirectly labeled antibody or other affinity reagent.

公开(公告)号：US20220162693A1

公开(公告)日：2022-05-26

申请号：US17291962

申请日：2019-11-11

Applicant: MGI Tech Co., Ltd. ,  BGI Shenzhen

Inventor： Snezana Drmanac ,  Handong Li ,  Matthew J. Callow ,  Leon Eckhardt ,  Scott Gablenz ,  Radoje Drmanac ,  Ping Zhou

IPC: C12Q1/6874 ,  C12Q1/6876

Abstract: The invention provides compositions and methods for sequencing nucleic acids and other applications. In sequencing by synthesis, unlabeled reversible terminators are incorporated by a polymerase in each cycle, then labeled after incorporation by binding to the reversible terminator a directly or indirectly labeled antibody or other affinity reagent.

公开(公告)号：US20210254150A1

公开(公告)日：2021-08-19

申请号：US17084482

申请日：2020-10-29

Applicant: MGI Tech Co., Ltd. ,  BGI Shenzhen

Inventor： Radoje Drmanac ,  Snezana Drmanac ,  Handong Li ,  Xun Xu ,  Matthew J. Callow ,  Leon Eckhardt ,  Naibo Yang

IPC: C12Q1/6874 ,  C12Q1/6876 ,  C07K16/44 ,  C12Q1/6804 ,  A61K47/00 ,  C12Q1/6869 ,  C07K16/28

Abstract: The invention provides compositions and methods for sequencing nucleic acids and other applications. In sequencing by synthesis, unlabeled reversible terminators are incorporated by a polymerase in each cycle, then labeled after incorporation by binding to the reversible terminator a directly or indirectly labeled antibody or other affinity reagent.

公开(公告)号：US10851410B2

公开(公告)日：2020-12-01

申请号：US15862566

申请日：2018-01-04

Applicant: MGI Tech Co., Ltd. ,  BGI Shenzhen

Inventor： Radoje Drmanac ,  Snezana Drmanac ,  Handong Li ,  Xun Xu ,  Matthew J. Callow ,  Leon Eckhardt ,  Naibo Yang

IPC: C12P19/34 ,  C12Q1/6874 ,  C12Q1/6876 ,  C07K16/44 ,  C12Q1/6804 ,  A61K47/00 ,  C12Q1/6869 ,  C07K16/28

Abstract: The invention provides compositions and methods for sequencing nucleic acids and other applications. In sequencing by synthesis, unlabeled reversible terminators are incorporated by a polymerase in each cycle, then labeled after incorporation by binding to the reversible terminator a directly or indirectly labeled antibody or other affinity reagent.