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公开(公告)号：US20180223358A1
公开(公告)日：2018-08-09
申请号：US15862566
申请日：2018-01-04
发明人： Radoje Drmanac , Snezana Drmanac , Handong Li , Xun Xu , Matthew J. Callow , Leon Eckhardt , Naibo Yang , Quan Ding
IPC分类号： C12Q1/6874 , C12Q1/6876
CPC分类号： C12Q1/6874 , A61K47/00 , C07K16/2851 , C07K16/44 , C07K2317/73 , C12Q1/6804 , C12Q1/6869 , C12Q1/6876 , C12Q2525/186
摘要： The invention provides compositions and methods for sequencing nucleic acids and other applications. In sequencing by synthesis, unlabeled reversible terminators are incorporated by a polymerase in each cycle, then labeled after incorporation by binding to the reversible terminator a directly or indirectly labeled antibody or other affinity reagent.
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公开(公告)号：US20190144482A1
公开(公告)日：2019-05-16
申请号：US16094845
申请日：2017-04-21
发明人： Handong Li , Snezana Drmanac , Radoje Drmanac , Xun Xu , Lingling Peng , Scott Gablenz
IPC分类号： C07H15/04 , C07H19/10 , C07H21/04 , C07H19/20 , C12Q1/6869
摘要： Reversibly blocked nucleoside analogues and methods of using such nucleoside analogues for sequencing of nucleic acids are provided.
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公开(公告)号：US10988501B2
公开(公告)日：2021-04-27
申请号：US16094845
申请日：2017-04-21
发明人： Handong Li , Snezana Drmanac , Radoje Drmanac , Xun Xu , Lingling Peng , Scott Gablenz
IPC分类号： C07H19/10 , C07H19/20 , C07H15/04 , C12Q1/6869 , C07H21/04
摘要： Reversibly blocked nucleoside analogues and methods of using such nucleoside analogues for sequencing of nucleic acids are provided.
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公开(公告)号：US20240301486A1
公开(公告)日：2024-09-12
申请号：US18592885
申请日：2024-03-01
IPC分类号： C12Q1/6874 , C07H21/04 , C07K1/04 , C12Q1/6806 , C12Q1/682 , C12Q1/6837 , C12Q1/6869 , G01N15/1404 , G01N15/1434
CPC分类号： C12Q1/6874 , C07H21/04 , C07K1/047 , C12Q1/6806 , C12Q1/682 , C12Q1/6837 , C12Q1/6869 , G01N15/1404 , G01N15/1434 , C12Q2525/151 , C12Q2525/313 , C12Q2531/125 , C12Q2565/513 , Y10S977/778 , Y10S977/789 , Y10S977/792 , Y10S977/88 , Y10S977/882
摘要： Random arrays of single molecules are provided for carrying out large scale analyzes, particularly of biomolecules, such as genomic DNA, cDNAs, proteins, and the like. In one aspect, arrays of the invention comprise concatemers of DNA fragments that are randomly disposed on a regular array of discrete spaced apart regions, such that substantially all such regions contain no more than a single concatemer. Preferably, such regions have areas substantially less than 1 μm2 and have nearest neighbor distances that permit optical resolution of on the order of 109 single molecules per cm2. Many analytical chemistries can be applied to random arrays of the invention, including sequencing by hybridization chemistries, sequencing by synthesis chemistries, SNP detection chemistries, and the like, to greatly expand the scale and potential applications of such techniques.
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公开(公告)号：US20190271037A1
公开(公告)日：2019-09-05
申请号：US16297379
申请日：2019-03-08
发明人： Rongqin Ke , Snezana Drmanac , Radoje Drmanac , Guangyang Cai , Matthew Callow
IPC分类号： C12Q1/6874 , C12Q1/6806
摘要： This application discloses methods of producing a DNA strand for sequencing, as well as genetic constructs, libraries, and arrays using DNA strands produced according to these methods. The application also discloses methods of sequencing using the DNA strands, genetic constructs, libraries, and arrays produced. In certain aspects, DNA being sequenced includes a target sequence and at least one adaptor sequence.
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6.发明申请SIGNAL CONFINEMENT SEQUENCING (SCS) AND NUCLEOTIDE ANALOGUES FOR SIGNAL CONFINEMENT SEQUENCING 审中-公开信号约束测序（SCS）和信号约束测序的核苷酸类似物公开(公告)号：US20160130647A1
公开(公告)日：2016-05-12
申请号：US14921466
申请日：2015-10-23
发明人： Snezana Drmanac , Handong Li , Radoje Drmanac , Eric Harness , Chongjun Xu
IPC分类号： C12Q1/68 , C07H19/10 , C07H19/207
CPC分类号： C12Q1/6874 , C07H19/10 , C07H19/20 , C07H19/207 , C12Q1/6869 , C12Q2525/113 , C12Q2563/131
摘要： Novel fluorescent nucleotide analogues are provided herein. Also provided herein are methods of using the nucleotide analogues in sequencing-by-synthesis and signal confinement methods.
摘要翻译： 本文提供了新的荧光核苷酸类似物。 本文还提供了在合成序列和信号限制方法中使用核苷酸类似物的方法。
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公开(公告)号：US10351909B2
公开(公告)日：2019-07-16
申请号：US15442659
申请日：2017-02-25
IPC分类号： C12Q1/68 , C12Q1/6874 , C12Q1/682 , C12Q1/6837 , C12Q1/6869 , C07H21/04 , C07K1/04 , G01N15/14 , C12Q1/6806
摘要： The invention relates to an automated method for high-throughput DNA sequencing from high density DNA arrays by (a) initiating a first sequencing reaction on a first high density DNA array; and imaging said first high density DNA array using a detector, and (b) initiating a first sequencing reaction on a second high density DNA array; and imaging said second high density DNA array using the detector, wherein the first sequencing reaction in (a) is initiated before the first sequencing reaction in (b) is initiated such that the sequencing reactions in (a) and (b) are staggered. By using asynchronous sequencing reactions and imaging two separate arrays using one detector, imaging can be carried out on one array while sequencing reactions are carried out on one the other, substrate, the other substrate is imaged, reducing the idle time of the imaging system.
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公开(公告)号：US10227647B2
公开(公告)日：2019-03-12
申请号：US15040906
申请日：2016-02-10
发明人： Rongqin Ke , Snezana Drmanac , Radoje Drmanac , Guangyang Cai , Matthew Callow
IPC分类号： C12Q1/68 , C12Q1/6874 , C12Q1/6806
摘要： This application discloses methods of producing a DNA strand for sequencing, as well as genetic constructs, libraries, and arrays using DNA strands produced according to these methods. The application also discloses methods of sequencing using the DNA strands, genetic constructs, libraries, and arrays produced. In certain aspects, DNA being sequenced includes a target sequence and at least one adaptor sequence.
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公开(公告)号：US09944984B2
公开(公告)日：2018-04-17
申请号：US15425791
申请日：2017-02-06
CPC分类号： C12Q1/6874 , C07H21/04 , C07K1/047 , C12Q1/6806 , C12Q1/682 , C12Q1/6837 , C12Q1/6869 , C12Q2525/151 , C12Q2525/313 , C12Q2531/125 , C12Q2565/513 , G01N15/1404 , G01N15/1434 , Y10S977/778 , Y10S977/789 , Y10S977/792 , Y10S977/88 , Y10S977/882 , C12Q2521/307 , C12Q2525/161 , C12Q2535/122 , C12Q2563/179 , C12Q2565/514
摘要： A high density DNA array comprising a patterned surface, said surface comprising a pattern of small DNA binding regions separated by a non-DNA binding surface, wherein the DNA binding regions comprise DNA capture chemistry and the non-DNA binding surface does not have the DNA capture chemistry wherein more than 50% of the DNA binding regions in the array have single informative DNA species.
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公开(公告)号：US09650673B2
公开(公告)日：2017-05-16
申请号：US14714133
申请日：2015-05-15
CPC分类号： C12Q1/6874 , C07H21/04 , C07K1/047 , C12Q1/6806 , C12Q1/682 , C12Q1/6837 , C12Q1/6869 , C12Q2525/151 , C12Q2525/313 , C12Q2531/125 , C12Q2565/513 , G01N15/1404 , G01N15/1434 , Y10S977/778 , Y10S977/789 , Y10S977/792 , Y10S977/88 , Y10S977/882 , C12Q2521/307 , C12Q2525/161 , C12Q2535/122 , C12Q2563/179 , C12Q2565/514
摘要： Random arrays of single molecules are provided for carrying out large scale analyzes, particularly of biomolecules, such as genomic DNA, cDNAs, proteins, and the like. In one aspect, arrays of the invention comprise concatemers of DNA fragments that are randomly disposed on a regular array of discrete spaced apart regions, such that substantially all such regions contain no more than a single concatemer. Preferably, such regions have areas substantially less than 1 μm2 and have nearest neighbor distances that permit optical resolution of on the order of 109 single molecules per cm2. Many analytical chemistries can be applied to random arrays of the invention, including sequencing by hybridization chemistries, sequencing by synthesis chemistries, SNP detection chemistries, and the like, to greatly expand the scale and potential applications of such techniques.
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