公开(公告)号：US12195493B2

公开(公告)日：2025-01-14

申请号：US17208831

申请日：2021-03-22

Applicant: MGI Tech Co., Ltd. ,  BGI Shenzhen

Inventor： Handong Li ,  Snezana Drmanac ,  Radoje Drmanac ,  Xun Xu ,  Lingling Peng ,  Scott Gablenz

IPC: C07H15/04 ,  C07H19/10 ,  C07H19/20 ,  C07H21/04 ,  C12Q1/6869

Abstract: Reversibly blocked nucleoside analogues and methods of using such nucleoside analogues for sequencing of nucleic acids are provided.

公开(公告)号：US20240117425A1

公开(公告)日：2024-04-11

申请号：US18462652

申请日：2023-09-07

Applicant: MGI Tech Co., Ltd. ,  BGI Shenzhen

Inventor： Radoje Drmanac ,  Snezana Drmanac ,  Handong Li ,  Xun Xu ,  Matthew J. Callow ,  Leon Eckhardt ,  Naibo Yang

IPC: C12Q1/6874 ,  A61K47/00 ,  C07K16/28 ,  C07K16/44 ,  C12Q1/6804 ,  C12Q1/6869 ,  C12Q1/6876

CPC classification number: C12Q1/6874 ,  A61K47/00 ,  C07K16/2851 ,  C07K16/44 ,  C12Q1/6804 ,  C12Q1/6869 ,  C12Q1/6876 ,  C07K2317/73

Abstract: The invention provides compositions and methods for sequencing nucleic acids and other applications. In sequencing by synthesis, unlabeled reversible terminators are incorporated by a polymerase in each cycle, then labeled after incorporation by binding to the reversible terminator a directly or indirectly labeled antibody or other affinity reagent.

公开(公告)号：US11788138B2

公开(公告)日：2023-10-17

申请号：US17084482

申请日：2020-10-29

Applicant: MGI Tech Co., Ltd. ,  BGI Shenzhen

Inventor： Radoje Drmanac ,  Snezana Drmanac ,  Handong Li ,  Xun Xu ,  Matthew J. Callow ,  Leon Eckhardt ,  Naibo Yang

IPC: C12P19/34 ,  C12Q1/6874 ,  C12Q1/6876 ,  C07K16/44 ,  C12Q1/6804 ,  A61K47/00 ,  C12Q1/6869 ,  C07K16/28

CPC classification number: C12Q1/6874 ,  A61K47/00 ,  C07K16/2851 ,  C07K16/44 ,  C12Q1/6804 ,  C12Q1/6869 ,  C12Q1/6876 ,  C07K2317/73 ,  C12Q1/6804 ,  C12Q2525/186 ,  C12Q1/6869 ,  C12Q2525/186

Abstract: The invention provides compositions and methods for sequencing nucleic acids and other applications. In sequencing by synthesis, unlabeled reversible terminators are incorporated by a polymerase in each cycle, then labeled after incorporation by binding to the reversible terminator a directly or indirectly labeled antibody or other affinity reagent.

公开(公告)号：US20210206794A1

公开(公告)日：2021-07-08

申请号：US17208831

申请日：2021-03-22

Applicant: MGI Tech Co., Ltd. ,  BGI Shenzhen

Inventor： Handong Li ,  Snezana Drmanac ,  Radoje Drmanac ,  Xun Xu ,  Lingling Peng ,  Scott Gablenz

IPC: C07H15/04 ,  C07H19/10 ,  C07H19/20 ,  C12Q1/6869 ,  C07H21/04

Abstract: Reversibly blocked nucleoside analogues and methods of using such nucleoside analogues for sequencing of nucleic acids are provided.

公开(公告)号：US20220162693A1

公开(公告)日：2022-05-26

申请号：US17291962

申请日：2019-11-11

Applicant: MGI Tech Co., Ltd. ,  BGI Shenzhen

Inventor： Snezana Drmanac ,  Handong Li ,  Matthew J. Callow ,  Leon Eckhardt ,  Scott Gablenz ,  Radoje Drmanac ,  Ping Zhou

IPC: C12Q1/6874 ,  C12Q1/6876

Abstract: The invention provides compositions and methods for sequencing nucleic acids and other applications. In sequencing by synthesis, unlabeled reversible terminators are incorporated by a polymerase in each cycle, then labeled after incorporation by binding to the reversible terminator a directly or indirectly labeled antibody or other affinity reagent.

公开(公告)号：US20210254150A1

公开(公告)日：2021-08-19

申请号：US17084482

申请日：2020-10-29

Applicant: MGI Tech Co., Ltd. ,  BGI Shenzhen

Inventor： Radoje Drmanac ,  Snezana Drmanac ,  Handong Li ,  Xun Xu ,  Matthew J. Callow ,  Leon Eckhardt ,  Naibo Yang

IPC: C12Q1/6874 ,  C12Q1/6876 ,  C07K16/44 ,  C12Q1/6804 ,  A61K47/00 ,  C12Q1/6869 ,  C07K16/28

Abstract: The invention provides compositions and methods for sequencing nucleic acids and other applications. In sequencing by synthesis, unlabeled reversible terminators are incorporated by a polymerase in each cycle, then labeled after incorporation by binding to the reversible terminator a directly or indirectly labeled antibody or other affinity reagent.

公开(公告)号：US10851410B2

公开(公告)日：2020-12-01

申请号：US15862566

申请日：2018-01-04

Applicant: MGI Tech Co., Ltd. ,  BGI Shenzhen

Inventor： Radoje Drmanac ,  Snezana Drmanac ,  Handong Li ,  Xun Xu ,  Matthew J. Callow ,  Leon Eckhardt ,  Naibo Yang

IPC: C12P19/34 ,  C12Q1/6874 ,  C12Q1/6876 ,  C07K16/44 ,  C12Q1/6804 ,  A61K47/00 ,  C12Q1/6869 ,  C07K16/28

Abstract: The invention provides compositions and methods for sequencing nucleic acids and other applications. In sequencing by synthesis, unlabeled reversible terminators are incorporated by a polymerase in each cycle, then labeled after incorporation by binding to the reversible terminator a directly or indirectly labeled antibody or other affinity reagent.

公开(公告)号：US20230295696A1

公开(公告)日：2023-09-21

申请号：US18006375

申请日：2020-07-29

Applicant: MGI Tech Co., Ltd.

Inventor： Jay Shafto ,  Radoje Drmanac ,  Snezana Drmanac ,  Chongjun Xu ,  Meihua Gong ,  Ping Wang ,  Xiaojuan Long ,  Wei Zhao ,  Huan Luo ,  Hui Jiang ,  Jian Liu

IPC: C12Q1/6834 ,  C12Q1/686 ,  C40B50/18

CPC classification number: C12Q1/6834 ,  C12Q1/686 ,  C40B50/18 ,  C12Q2600/16

Abstract: Provided are a method for loading a nucleic acid molecule, for example nucleic acid nanoball (e.g., DNA nanoball (DNB)), on a solid support, and a kit for the method.

公开(公告)号：US20240240174A1

公开(公告)日：2024-07-18

申请号：US18575985

申请日：2022-07-22

Applicant: MGI Tech Co., Ltd.

Inventor： Radoje T. Drmanac ,  Brock A. Peters ,  Andrei Alexeev ,  Snezana Drmanac ,  Amulya Nanisetti

IPC: C12N15/10

CPC classification number: C12N15/1065 ,  C12N15/1068

Abstract: This application relates to methods and compositions for preparing a library of polynucleotides for sequencing comprises in a single reaction mixture. The method comprises contacting a double-stranded target nucleic acid with one or more nicking agents to produce overlapping nucleic acid fragments separated by staggered single-stranded breaks; and contacting a partially double-stranded first adapter with at least one of the nucleic acid fragments in the presence of a ligase, thereby ligating the 5′ terminus of the double-stranded region of the first adapter to the 3′ terminus of the at least one of the nucleic acid fragments using a DNA ligase via 3′ branch ligation. The first adapter comprises (i) a double-stranded blunt end having a 5′ terminus and a 3′ terminus and (ii) a single-stranded region comprising a barcode.

公开(公告)号：US20240043924A1

公开(公告)日：2024-02-08

申请号：US18361020

申请日：2023-07-28

Applicant: MGI Tech Co., Ltd.

Inventor： Radoje T. Drmanac ,  Brock A. Peters ,  Snezana Drmanac

IPC: C12Q1/6874

CPC classification number: C12Q1/6874

Abstract: Disclosed herein are DNA sequencing methods involving applying a long DNA molecule to an indexed array comprising an array of transfer sites (TS). Each TS comprises a substrate and a source of clonal barcodes (SCB) attached to or situated on the substrate. Each SCB comprises many copies of a unique transferable barcode sequence, and the unique transferable barcode sequence associated with each TS is known. The unique transferable barcode sequence is transferred from the SCB portion of the TS to a location on the long DNA molecule. DNA fragments comprising the barcode sequences are recovered from the array and sequenced. Sequence reads from these fragments are assembled based on the relative positions of the TS on the array.