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    TARGETED DELIVERY OF REAGENTS TO SPOTS ON A PLANAR SUPPORT THROUGH PATTERNED TRANSFER SHEETS
    11.
    发明申请
    TARGETED DELIVERY OF REAGENTS TO SPOTS ON A PLANAR SUPPORT THROUGH PATTERNED TRANSFER SHEETS 有权
    通过图案转让表对平面图支持的试剂进行指定的交付

    公开(公告)号：US20140027284A1

    公开(公告)日：2014-01-30

    申请号：US13950590

    申请日：2013-07-25

    Applicant: Bio-Rad Laboratories, Inc.

    Inventor： Clayton T. McKee ,  William Strong

    IPC: G01N27/447

    CPC classification number: G01N27/44739 ,  G01N27/44747 ,  G01N33/54306 ,  G01N33/54393

    Abstract: Molecular species that are immobilized in discrete locations on a planar support such as protein bands on a gel or a blotting membrane or species applied in dots or spots on a membrane are reacted with binding reagents that are applied through a porous hydrophilic transfer sheet placed over the planar support, the sheet having at least one region that is laterally bordered by a barrier with the binding reagent retained within the bordered region. The bordered region is placed directly over an area on the planar support where the molecular species are expected to reside if they are present on the support. The binding reagent is then delivered into the support to contact the species. Targeted delivery of the binding reagent is thus achieved with improved efficiency.

    Abstract translation: 固定在平面载体上的离散位置上的分子物质如凝胶或印迹膜上的蛋白质条带或涂在膜上的点或点上的物质与通过多孔亲水转移片施加的结合试剂反应， 所述片材具有至少一个区域，所述至少一个区域被阻挡物侧向界定，所述粘合剂保留在所述边界区域内。 如果分子种类存在于支持体上，则边界区域直接放置在平面支撑体上的分子种类预期驻留的区域上。 然后将结合试剂输送到载体中以接触物种。 因此，以提高的效率实现了结合试剂的靶向递送。

    Protein quantitation device
    12.
    发明授权
    Protein quantitation device 有权

    公开(公告)号：US10948403B2

    公开(公告)日：2021-03-16

    申请号：US16151732

    申请日：2018-10-04

    Applicant: Bio-Rad Laboratories, Inc.

    Inventor： Evan Thrush ,  Steven Swihart ,  William Strong ,  Trey Marlowe ,  Li Lu

    IPC: G01N21/31 ,  G01N21/3577 ,  G01N21/3563

    Abstract: Systems for protein quantitation using a Fabry-Perot interferometer. In one arrangement, a quantitation device includes an infrared source, a sample holder, and a Fabry-Perot interferometer positioned to receive infrared radiation from the source passing through a sample on the sample holder. A band pass optical filter sets the working range of the interferometer, and radiation exiting the interferometer falls on a detector that produces a signal indicating the intensity of the received radiation. A controller causes the interferometer to be tuned to a number of different resonance wavelengths and receives the intensity signals, for determination of an absorbance spectrum.

    Dip-stick western blot
    14.
    发明授权
    Dip-stick western blot 有权

    公开(公告)号：US10852273B2

    公开(公告)日：2020-12-01

    申请号：US16138860

    申请日：2018-09-21

    Applicant: Bio-Rad Laboratories, Inc.

    Inventor： William Strong

    IPC: G01N27/447 ,  G01N33/543 ,  G01N30/90

    Abstract: Methods, kits, and systems are provided for separating, immobilizing, and/or detecting analytes of one or more samples using dipsticks. A ‘dipstick’ is an object that can be embedded and subsequently removed from a separation medium, and to which analytes can be immobilized while the object is embedded in the separation medium. Examples of separation media include an electrophoresis gel of any format and a stationary phase for column chromatography. Embodiments of the present methods include applying a sample to a separation medium; separating analytes of the sample in the separation medium along a separation axis; immobilizing the analytes on a dipstick embedded in the separation medium; removing the dipstick from the separation medium; and detecting the analytes immobilized on the removed dipstick.

    AUTOMATED BLOTTING USING SLIDING DEVICES
    17.
    发明申请
    AUTOMATED BLOTTING USING SLIDING DEVICES 有权
    使用滑动装置的自动吹瓶

    公开(公告)号：US20150253251A1

    公开(公告)日：2015-09-10

    申请号：US14642487

    申请日：2015-03-09

    Applicant: Bio-Rad Laboratories, Inc.

    Inventor： Clayton T. McKee ,  William Strong

    IPC: G01N21/76 ,  G01N21/03

    CPC classification number: B01L3/502738 ,  B01L2200/0605 ,  B01L2200/0642 ,  B01L2300/045 ,  B01L2300/047 ,  B01L2300/0816 ,  B01L2300/0874 ,  B01L2400/0415 ,  B01L2400/0418 ,  B01L2400/0421 ,  B01L2400/0487 ,  B01L2400/065 ,  C12Q1/6806 ,  G01N27/44717 ,  G01N27/44739 ,  G01N33/54366

    Abstract: Devices, systems, methods, and kits are provided for performing separation, immobilization, blotting, and/or detection of analytes from biological samples. In some embodiments, the devices are constructed from two solid substrates with surfaces in contact. The devices include a plurality of channels formed from indentations in these surfaces. The indentations can be aligned with each other across the interface between the substrates, and realigned by shifting or sliding one substrate relative to the other. In some embodiments, the devices are constructed from three layers of a solid substrate. A separation channel in the middle layer of the device is first used for analyte separation. The middle layer can then be slid relative the top and/or bottom layer, thereby aligning the separation channel with a blotting membrane. Analytes can then be transferred to the membrane using electrodes in the top and bottom layers.

    Abstract translation: 提供了设备，系统，方法和试剂盒，用于进行生物样品中分析物的分离，固定，印迹和/或检测。 在一些实施例中，装置由具有接触表面的两个固体基板构成。 这些装置包括从这些表面中的凹陷形成的多个通道。 凹陷可以在基板之间的界面上彼此对齐，并且通过相对于另一个移动或滑动一个基板来重新对准。 在一些实施例中，装置由三层固体基板构成。 器件中间层的分离通道首先用于分析物分离。 然后中间层可以相对于顶层和/或底层滑动，从而使分离通道与印迹膜对准。 然后可以使用顶层和底层中的电极将分析物转移到膜。

    MINI-GEL COMB
    18.
    发明申请
    MINI-GEL COMB 审中-公开
    迷你凝胶

    公开(公告)号：US20150153306A1

    公开(公告)日：2015-06-04

    申请号：US14558541

    申请日：2014-12-02

    Applicant: Bio-Rad Laboratories, Inc.

    Inventor： William Strong

    IPC: G01N27/447

    CPC classification number: G01N27/44704

    Abstract: Molds for casting and retaining electrophoresis gel strips are provided, along with methods, kits, and systems for performing electrophoresis, electroelution, and/or electroblotting using these molds.

    Abstract translation: 提供了用于铸造和保留电泳胶条的模具以及使用这些模具进行电泳，电洗脱和/或电印迹的方法，试剂盒和系统。

    Protein quantitation device
    20.
    发明授权
    Protein quantitation device 有权

    公开(公告)号：US11435285B2

    公开(公告)日：2022-09-06

    申请号：US17199312

    申请日：2021-03-11

    Applicant: Bio-Rad Laboratories, Inc.

    Inventor： Evan Thrush ,  Steven Swihart ,  William Strong ,  Trey Marlowe ,  Li Lu

    IPC: G01N21/31 ,  G01N21/3577 ,  G01N21/3563

    Abstract: Systems for protein quantitation using a Fabry-Perot interferometer. In one arrangement, a quantitation device includes an infrared source, a sample holder, and a Fabry-Perot interferometer positioned to receive infrared radiation from the source passing through a sample on the sample holder. A band pass optical filter sets the working range of the interferometer, and radiation exiting the interferometer falls on a detector that produces a signal indicating the intensity of the received radiation. A controller causes the interferometer to be tuned to a number of different resonance wavelengths and receives the intensity signals, for determination of an absorbance spectrum.

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