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公开(公告)号：US20120271037A1
公开(公告)日：2012-10-25
申请号：US13488840
申请日：2012-06-05
IPC分类号： C07K1/34
摘要： The expression vectors and methods using an E. coli expression system for the large scale production of IL-29 are described. The vectors utilize the IL-29 coding sequence with specific changes in nucleotides in order to optimize codons and mRNA secondary structure for translation in E. coli. Also included are methods of producing, purifying and pegylating an IL-29 polypeptide.
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公开(公告)号：US08211670B2
公开(公告)日：2012-07-03
申请号：US13114566
申请日：2011-05-24
摘要： The expression vectors and methods using an E. coli expression system for the large scale production of IL-29 are described. The vectors utilize the IL-29 coding sequence with specific changes in nucleotides in order to optimize codons and mRNA secondary structure for translation in E. coli. Also included are methods of producing, purifying and pegylating an IL-29 polypeptide.
摘要翻译： 描述了用于大规模生产IL-29的大肠杆菌表达系统的表达载体和方法。 载体利用具有核苷酸特异性变化的IL-29编码序列，以优化用于大肠杆菌翻译的密码子和mRNA二级结构。 还包括产生，纯化和聚乙二醇化IL-29多肽的方法。
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公开(公告)号：US20100222552A1
公开(公告)日：2010-09-02
申请号：US12701358
申请日：2010-02-05
IPC分类号： C07K14/54
摘要： The expression vectors and methods using an E. coli expression system for the large scale production of IL-29 are described. The vectors utilize the IL-29 coding sequence with specific changes in nucleotides in order to optimize codons and mRNA secondary structure for translation in E. coli. Also included are methods of producing, purifying and pegylating an IL-29 polypeptide.
摘要翻译： 描述了用于大规模生产IL-29的大肠杆菌表达系统的表达载体和方法。 载体利用具有核苷酸特异性变化的IL-29编码序列，以优化用于大肠杆菌翻译的密码子和mRNA二级结构。 还包括产生，纯化和聚乙二醇化IL-29多肽的方法。
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公开(公告)号：US07745176B2
公开(公告)日：2010-06-29
申请号：US11539036
申请日：2006-10-05
申请人： Bruce L. Zamost , Douglas C. Covert
发明人： Bruce L. Zamost , Douglas C. Covert
CPC分类号： C07K14/54
摘要： The expression vectors and methods using an E. coli expression system for the large scale production of IL-21 are described. The vectors utilize the IL-21 coding sequence with specific changes in nucleotides in order to optimize codons and mRNA secondary structure for translation in E. coli. Using the expression vectors, the IL-21 gene was produced in E. coli to a level of greater than 1 g/L in fed batch fermentation. Also included are OmpT deficient E. coli strains transformed with an IL-21 expression vector.
摘要翻译： 描述了用于大规模生产IL-21的大肠杆菌表达系统的表达载体和方法。 载体利用具有核苷酸特异性变化的IL-21编码序列，以优化用于大肠杆菌翻译的密码子和mRNA二级结构。 使用表达载体，在补料分批发酵中，在大肠杆菌中产生IL-21基因至大于1g / L的水平。 还包括用IL-21表达载体转化的OmpT缺陷型大肠杆菌菌株。
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公开(公告)号：US20100003722A1
公开(公告)日：2010-01-07
申请号：US12537935
申请日：2009-08-07
IPC分类号： C12P21/04
摘要： The expression vectors and methods using an E. coli expression system for the large scale production of IL-29 are described. The vectors utilize the IL-29 coding sequence with specific changes in nucleotides in order to optimize codons and mRNA secondary structure for translation in E. coli. Also included are methods of producing, purifying and pegylating an IL-29 polypeptide.
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