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公开(公告)号：US20080096252A1
公开(公告)日：2008-04-24
申请号：US11538688
申请日：2006-10-04
摘要： The expression vectors and methods using an E. Coli expression system for the large scale production of IL-29 are described. The vectors utilize the IL-29 coding sequence with specific changes in nucleotides in order to optimize codons and mRNA secondary structure for translation in E. coli. Also included are methods of producing, purifying and pegylating an IL-29 polypeptide.
摘要翻译： 描述了用于大规模生产IL-29的大肠杆菌表达系统的表达载体和方法。 载体利用具有核苷酸特异性变化的IL-29编码序列，以优化用于大肠杆菌翻译的密码子和mRNA二级结构。 还包括产生，纯化和聚乙二醇化IL-29多肽的方法。
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公开(公告)号：US20100003722A1
公开(公告)日：2010-01-07
申请号：US12537935
申请日：2009-08-07
IPC分类号： C12P21/04
摘要： The expression vectors and methods using an E. coli expression system for the large scale production of IL-29 are described. The vectors utilize the IL-29 coding sequence with specific changes in nucleotides in order to optimize codons and mRNA secondary structure for translation in E. coli. Also included are methods of producing, purifying and pegylating an IL-29 polypeptide.
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公开(公告)号：US07759092B2
公开(公告)日：2010-07-20
申请号：US12537935
申请日：2009-08-07
摘要： The expression vectors and methods using an E. coli expression system for the large scale production of IL-29 are described. The vectors utilize the IL-29 coding sequence with specific changes in nucleotides in order to optimize codons and mRNA secondary structure for translation in E. coli. Also included are methods of producing, purifying and pegylating an IL-29 polypeptide.
摘要翻译： 描述了用于大规模生产IL-29的大肠杆菌表达系统的表达载体和方法。 载体利用具有核苷酸特异性变化的IL-29编码序列，以优化用于大肠杆菌翻译的密码子和mRNA二级结构。 还包括产生，纯化和聚乙二醇化IL-29多肽的方法。
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公开(公告)号：US07968315B2
公开(公告)日：2011-06-28
申请号：US12701358
申请日：2010-02-05
摘要： The expression vectors and methods using an E. coli expression system for the large scale production of IL-29 are described. The vectors utilize the IL-29 coding sequence with specific changes in nucleotides in order to optimize codons and mRNA secondary structure for translation in E. coli. Also included are methods of producing, purifying and pegylating an IL-29 polypeptide.
摘要翻译： 描述了用于大规模生产IL-29的大肠杆菌表达系统的表达载体和方法。 载体利用具有核苷酸特异性变化的IL-29编码序列，以优化用于大肠杆菌翻译的密码子和mRNA二级结构。 还包括产生，纯化和聚乙二醇化IL-29多肽的方法。
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公开(公告)号：US20120015403A1
公开(公告)日：2012-01-19
申请号：US13114566
申请日：2011-05-24
IPC分类号： C12P21/02
摘要： The expression vectors and methods using an E. coli expression system for the large scale production of IL-29 are described. The vectors utilize the IL-29 coding sequence with specific changes in nucleotides in order to optimize codons and mRNA secondary structure for translation in E. coli. Also included are methods of producing, purifying and pegylating an IL-29 polypeptide.
摘要翻译： 描述了用于大规模生产IL-29的大肠杆菌表达系统的表达载体和方法。 载体利用具有核苷酸特异性变化的IL-29编码序列，以优化用于大肠杆菌翻译的密码子和mRNA二级结构。 还包括产生，纯化和聚乙二醇化IL-29多肽的方法。
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公开(公告)号：US20120271037A1
公开(公告)日：2012-10-25
申请号：US13488840
申请日：2012-06-05
IPC分类号： C07K1/34
摘要： The expression vectors and methods using an E. coli expression system for the large scale production of IL-29 are described. The vectors utilize the IL-29 coding sequence with specific changes in nucleotides in order to optimize codons and mRNA secondary structure for translation in E. coli. Also included are methods of producing, purifying and pegylating an IL-29 polypeptide.
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公开(公告)号：US08211670B2
公开(公告)日：2012-07-03
申请号：US13114566
申请日：2011-05-24
摘要： The expression vectors and methods using an E. coli expression system for the large scale production of IL-29 are described. The vectors utilize the IL-29 coding sequence with specific changes in nucleotides in order to optimize codons and mRNA secondary structure for translation in E. coli. Also included are methods of producing, purifying and pegylating an IL-29 polypeptide.
摘要翻译： 描述了用于大规模生产IL-29的大肠杆菌表达系统的表达载体和方法。 载体利用具有核苷酸特异性变化的IL-29编码序列，以优化用于大肠杆菌翻译的密码子和mRNA二级结构。 还包括产生，纯化和聚乙二醇化IL-29多肽的方法。
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公开(公告)号：US20100222552A1
公开(公告)日：2010-09-02
申请号：US12701358
申请日：2010-02-05
IPC分类号： C07K14/54
摘要： The expression vectors and methods using an E. coli expression system for the large scale production of IL-29 are described. The vectors utilize the IL-29 coding sequence with specific changes in nucleotides in order to optimize codons and mRNA secondary structure for translation in E. coli. Also included are methods of producing, purifying and pegylating an IL-29 polypeptide.
摘要翻译： 描述了用于大规模生产IL-29的大肠杆菌表达系统的表达载体和方法。 载体利用具有核苷酸特异性变化的IL-29编码序列，以优化用于大肠杆菌翻译的密码子和mRNA二级结构。 还包括产生，纯化和聚乙二醇化IL-29多肽的方法。
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公开(公告)号：US06258559B1
公开(公告)日：2001-07-10
申请号：US09532803
申请日：2000-03-21
申请人： Bruce L. Zamost
发明人： Bruce L. Zamost
IPC分类号： C12P2106
摘要： Methylotrophic yeasts are useful hosts for the production of commercially valuable recombinant proteins. However, the development of large-scale cultures of recombinant methylotrophic yeasts has been hindered by the formation of precipitation in culture media. A new soluble minimal medium overcomes this problem. Moreover, new feeding schemes provide cultures of high biomass, which produce biologically active recombinant protein.
摘要翻译： 甲基营养酵母是生产有价值的重组蛋白质的有用宿主。 然而，重组甲基营养酵母的大规模培养物的发育受到培养基中沉淀的形成的阻碍。 一种新的可溶性基本培养基克服了这个问题。 此外，新的饲养方案提供高生物量的培养物，其产生生物活性重组蛋白。
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10.发明授权Thermostable arabino furanoside produced by Bacillus stearothermophilus NRRL B-18659, Bacillus stearothermophilus NRRL B-18660 and Bacillus stearothermophilus NRRL B-18661 失效由嗜热脂肪芽孢杆菌NRRL B-18659，嗜热脂肪芽孢杆菌NRRL B-18660和嗜热脂肪芽孢杆菌NRRL B-18661产生的热稳定的阿拉伯呋喃糖苷
公开(公告)号：US5491087A
公开(公告)日：1996-02-13
申请号：US418044
申请日：1995-04-06
申请人： Bruce L. Zamost , Dana D. Elm
发明人： Bruce L. Zamost , Dana D. Elm
CPC分类号： C12Y302/01032 , C12N9/2482 , C12Y302/01008 , C12Y302/01037 , C12Y302/01055 , Y10S435/832
摘要： An isolated arabinofuranosidase from Bacillus stearothermophilus NRRL B-18659, Bacillus stearothermophilus NRRL B-18660 and Bacillus stearothermophilus NRRL B-18661 is disclosed. The arabinofuranosidase has a maximum activity at about pH 6.0 and at about 65.degree. C., maintains at least about 50% of its maximum activity at 70.degree. C. and pH 7.0 after 80 minutes, and has an isoelectric point of about 4.4. The arabinfuranosidase can be used in a method of hydrolyzing xylan present in wood pulp at temperatures of at least about 60.degree. C. and a pH of at least about 7.0. The arabinofuranosidase is used along with at least two xylanases and a xylosidase isolated from the above Bacillus stearothermophilus strains.
摘要翻译： 公开了来自嗜热脂肪芽孢杆菌NRRL B-18659，嗜热脂肪芽孢杆菌NRRL B-18660和嗜热脂肪芽孢杆菌NRRL B-18661的分离的阿拉伯呋喃糖苷酶。 阿拉伯呋喃糖苷酶在约pH 6.0和约65℃下具有最大活性，80分钟后在70℃和pH 7.0保持其最大活性的至少约50％，等电点约4.4。 阿拉伯呋喃糖苷酶可用于在至少约60℃的温度和至少约7.0的pH下水解木浆中存在的木聚糖的方法。 阿拉伯呋喃糖苷酶与至少两种木聚糖酶和从上述嗜热脂肪芽孢杆菌菌株分离的木糖苷酶一起使用。
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