Plant activation of insect toxin
    12.
    发明申请
    Plant activation of insect toxin 有权
    植物激活昆虫毒素

    公开(公告)号:US20050166284A1

    公开(公告)日:2005-07-28

    申请号:US11021115

    申请日:2004-12-22

    摘要: Compositions and methods for protecting a plant from an insect pest are provided. In particular, nucleic acid sequences encoding insect protoxins modified to comprise at least one proteolytic activation site that is sensitive to a plant protease or an insect gut protease are provided. Cleavage of the modified protoxin at the proteolytic activation site by a protease produces an active insect toxin. Methods of using the modified insect protoxin nucleic acid sequences and the polypeptides they encode to protect a plant from an insect pest are provided. Particular embodiments of the invention further provide modified insect protoxin compositions and formulations, expression cassettes, and transformed plants, plant cells, and seeds.

    摘要翻译: 提供了保护植物免受害虫害虫的组合物和方法。 特别地,提供了编码昆虫原毒素的核酸序列,其被修饰为包含对植物蛋白酶或昆虫肠道蛋白酶敏感的至少一个蛋白水解活化位点。 通过蛋白酶在蛋白水解活化位点切割修饰的原毒素会产生活性昆虫毒素。 提供了使用修饰的昆虫原毒素核酸序列及其编码的多肽保护植物免受害虫的方法。 本发明的具体实施方案还提供了改良的昆虫原毒素组合物和制剂,表达盒,以及转化的植物,植物细胞和种子。

    Methods and compositions for the expression of a polynucleotide of interest
    20.
    发明申请
    Methods and compositions for the expression of a polynucleotide of interest 审中-公开
    用于表达感兴趣的多核苷酸的方法和组合物

    公开(公告)号:US20070061917A1

    公开(公告)日:2007-03-15

    申请号:US11508045

    申请日:2006-08-22

    摘要: Methods and compositions for expressing a polynucleotide of interest are provided. Compositions comprise an enhancer domain set forth in SEQ ID NO: 1, 10, 15, or 16 and active variants and fragments thereof. Further provided are DNA constructs comprising at least one transcriptional enhancer sequence comprising the nucleotide sequence set forth in SEQ ID NO:1, 10, 15, or 16 or an active variant or fragment thereof, operably linked to a heterologous promoter. Such chimeric transcription regulatory regions can be operably linked any polynucleotide of interest. Further provided are cells, plants, plant parts, and germplasm comprising the DNA construct. Methods of using the chimeric transcriptional regulatory region are also provided. In specific embodiments, methods of expressing a polynucleotide of interest, including for example, sequences that confer tolerance to herbicides, and methods to select a cell having the DNA construct are provided.

    摘要翻译: 提供了表达感兴趣的多核苷酸的方法和组合物。 组合物包含SEQ ID NO:1,10,15或16所示的增强子结构域及其活性变体和片段。 还提供了包含至少一个转录增强子序列的DNA构建体,所述转录增强子序列包含可操作地连接到异源启动子的SEQ ID NO:1,0,15或16所示的核苷酸序列或其活性变体或片段。 这样的嵌合转录调控区域可以可操作地连接任何感兴趣的多核苷酸。 还提供了包含DNA构建体的细胞,植物,植物部分和种质。 还提供了使用嵌合转录调节区的方法。 在具体实施方案中,提供了表达目标多核苷酸的方法,包括例如赋予除草剂耐受性的序列,以及选择具有DNA构建体的细胞的方法。