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    Plant activation of insect toxin
    1.
    发明申请
    Plant activation of insect toxin 有权
    植物激活昆虫毒素

    公开(公告)号：US20050166284A1

    公开(公告)日：2005-07-28

    申请号：US11021115

    申请日：2004-12-22

    申请人： Andre Abad ,  Ronald Flannagan ,  Rafael Herrmann ,  Albert Lu ,  Billy McCutchen ,  James Presnail ,  Janet Rice ,  James Wong ,  Cao-Guo Yu

    发明人： Andre Abad ,  Ronald Flannagan ,  Rafael Herrmann ,  Albert Lu ,  Billy McCutchen ,  James Presnail ,  Janet Rice ,  James Wong ,  Cao-Guo Yu

    IPC分类号： A01H1/00 ,  A01H5/00 ,  C07K14/325 ,  C12N9/64 ,  C12N15/82

    CPC分类号： C12N15/8286 ,  C12N9/641 ,  Y02A40/162

    摘要： Compositions and methods for protecting a plant from an insect pest are provided. In particular, nucleic acid sequences encoding insect protoxins modified to comprise at least one proteolytic activation site that is sensitive to a plant protease or an insect gut protease are provided. Cleavage of the modified protoxin at the proteolytic activation site by a protease produces an active insect toxin. Methods of using the modified insect protoxin nucleic acid sequences and the polypeptides they encode to protect a plant from an insect pest are provided. Particular embodiments of the invention further provide modified insect protoxin compositions and formulations, expression cassettes, and transformed plants, plant cells, and seeds.

    摘要翻译： 提供了保护植物免受害虫害虫的组合物和方法。 特别地，提供了编码昆虫原毒素的核酸序列，其被修饰为包含对植物蛋白酶或昆虫肠道蛋白酶敏感的至少一个蛋白水解活化位点。 通过蛋白酶在蛋白水解活化位点切割修饰的原毒素会产生活性昆虫毒素。 提供了使用修饰的昆虫原毒素核酸序列及其编码的多肽保护植物免受害虫的方法。 本发明的具体实施方案还提供了改良的昆虫原毒素组合物和制剂，表达盒，以及转化的植物，植物细胞和种子。

    Genes encoding proteins with pesticidal activity
    3.
    发明申请
    Genes encoding proteins with pesticidal activity 有权
    编码具有杀虫活性的蛋白质的基因

    公开(公告)号：US20050261188A1

    公开(公告)日：2005-11-24

    申请号：US11108389

    申请日：2005-04-18

    申请人： Andre Abad ,  Ronald Flannagan ,  Rafael Herrmann ,  Theodore Kahn ,  Albert Lu ,  Billy McCutchen ,  James Presnail ,  James Wong ,  Cao-Guo Yu

    发明人： Andre Abad ,  Ronald Flannagan ,  Rafael Herrmann ,  Theodore Kahn ,  Albert Lu ,  Billy McCutchen ,  James Presnail ,  James Wong ,  Cao-Guo Yu

    IPC分类号： C07K14/325 ,  C12N15/32 ,  C12N15/82 ,  G06F19/00 ,  A01N63/00

    CPC分类号： C07K14/325 ,  C12N15/8286 ,  Y02A40/162

    摘要： The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding δ-endotoxins having pesticidal activity against insect pests. The invention further provides mutagenized nucleic acids that have been modified to encode pesticidal polypeptides such as endotoxins having improved pesticidal activity and/or altered pest specificity. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins that may be optimized as well as pesticidal compositions, expression cassettes, and transformed microorganisms and plants comprising a nucleic acid of the invention. These compositions find use in methods for controlling pests, especially plant pests.

    摘要翻译： 本发明提供从编码具有杀虫活性的昆虫害虫的δ-内毒素的苏云金芽孢杆菌菌株获得的核酸及其变体和片段。 本发明进一步提供已被修饰以编码杀虫多肽的诱变核酸，例如具有改善的杀虫活性和/或改变的有害生物特异性的内毒素。 本发明的具体实施方案提供编码可能被优化的杀虫蛋白质的分离的核酸，以及包含本发明的核酸的农药组合物，表达盒和转化的微生物和植物。 这些组合物可用于防治害虫，特别是植物害虫的方法。

    Genes encoding proteins with pesticidal activity
    5.
    发明申请
    Genes encoding proteins with pesticidal activity 有权
    编码具有杀虫活性的蛋白质的基因

    公开(公告)号：US20060021096A1

    公开(公告)日：2006-01-26

    申请号：US11224624

    申请日：2005-09-12

    申请人： Andre Abad ,  Hua Dong ,  Rafael Herrmann ,  Albert Lu ,  Billy McCutchen ,  Janet Rice ,  Eric Schepers ,  James Wong

    发明人： Andre Abad ,  Hua Dong ,  Rafael Herrmann ,  Albert Lu ,  Billy McCutchen ,  Janet Rice ,  Eric Schepers ,  James Wong

    IPC分类号： A01H5/00 ,  A01H1/00 ,  C07K14/415

    CPC分类号： C07K14/325 ,  C12N15/8286 ,  Y02A40/162

    摘要： Compositions and methods for protecting a plant from an insect pest are provided. The invention provides mutagenized nucleic acids that have been engineered to encode pesticidal polypeptides having increased resistance to proteolytic degradation by a plant protease. In particular, nucleic acid sequences encoding pesticidal polypeptides modified to comprise a proteolytic protection site that confers resistance to degradation or proteolytic inactivation by a plant protease are provided. Particular embodiments of the invention provide pesticidal polypeptide compositions and formulations, expression cassettes, and transformed plants, plant cells, and seeds. These compositions find use in methods for controlling pests, especially plant pests. Novel plant proteases, sequences encoding these proteases, and methods for their use are also provided.

    摘要翻译： 提供了保护植物免受害虫害虫的组合物和方法。 本发明提供已被工程化以编码具有增加的抗植物蛋白酶对蛋白水解降解的杀虫多肽的诱变的核酸。 特别地，提供了编码杀虫多肽的核酸序列，其被修饰为包含赋予植物蛋白酶降解或蛋白水解灭活抗性的蛋白水解保护位点。 本发明的具体实施方案提供杀虫多肽组合物和制剂，表达盒和转化植物，植物细胞和种子。 这些组合物可用于防治害虫，特别是植物害虫的方法。 还提供了新型植物蛋白酶，编码这些蛋白酶的序列及其使用方法。

    Methods and compositions for designing nucleic acid molecules for polypeptide expression in plants using plant virus codon-bias
    6.
    发明申请
    Methods and compositions for designing nucleic acid molecules for polypeptide expression in plants using plant virus codon-bias 审中-公开
    使用植物病毒密码子偏倚设计植物中多肽表达的核酸分子的方法和组合物

    公开(公告)号：US20060236424A1

    公开(公告)日：2006-10-19

    申请号：US11399028

    申请日：2006-04-05

    申请人： Andre Abad ,  Ronald Flannagan ,  Rafael Herrmann ,  Albert Lu ,  Billy McCutchen ,  Carl Simmons

    发明人： Andre Abad ,  Ronald Flannagan ,  Rafael Herrmann ,  Albert Lu ,  Billy McCutchen ,  Carl Simmons

    IPC分类号： A01H5/00 ,  A01H1/00 ,  C12N15/82 ,  C12N5/04

    CPC分类号： C12N15/8216 ,  C12N15/8286 ,  Y02A40/162

    摘要： The present invention relates to methods of designing nucleic acid molecules for improved expression of the encoded polypeptides in plants. In such methods, codon usage frequencies are biased towards codon usage frequencies of a plant virus, group of plant viruses, or a subset of nucleic acid molecules therefrom. In preferred embodiments, the encoded polypeptide affects the phenotype of the plant. The invention also pertains to nucleic acid molecules encoding insecticidal polypeptides wherein the nucleic acid molecules have been designed to have plant virus codon-biased. The invention also pertains to transgenic plants and progeny thereof with increased expression of insecticidal polypeptides for improved resistance to insects and other pests that are detrimental to plants of agricultural value.

    摘要翻译： 本发明涉及设计核酸分子以改进植物中编码多肽表达的方法。 在这种方法中，密码子使用频率偏向于植物病毒，植物病毒组或其核酸分子子集的密码子使用频率。 在优选的实施方案中，编码的多肽影响植物的表型。 本发明还涉及编码杀虫多肽的核酸分子，其中核酸分子被设计为具有植物病毒密码子偏倚。 本发明还涉及具有增加的杀虫多肽表达的转基因植物及其后代，以改善对对农业价值的植物有害的昆虫和其它害虫的抗性。