公开(公告)号：US20210290769A1

公开(公告)日：2021-09-23

申请号：US17264519

申请日：2019-08-01

Applicant: Carnegie Mellon University

Inventor： Alan J. Russell ,  Hironobu Murata ,  Sheiliza Carmali ,  Krzysztof Matyjaszewski

IPC: A61K47/58 ,  A61K38/47

Abstract: Materials and methods for targeted and controlled addition of polymers to proteins are provided herein. For example, the methods provided herein can include contacting a protein with (i) an inactive controlled radical polymerization (CRP) initiator blocker that can reacts with amino groups on the protein but cannot serve as an initiator for polymer addition, and (ii) an active CRP initiator that reacts with amino groups on the protein and also includes a group through which a polymer can be coupled to the initiator.

公开(公告)号：US10947525B2

公开(公告)日：2021-03-16

申请号：US16556665

申请日：2019-08-30

Applicant: Carnegie Mellon University

Inventor： Alan J. Russell ,  Hironobu Murata ,  Chad S. Cummings ,  Richard R. Koepsel

IPC: C12N9/96 ,  A61K38/46 ,  C12N9/16 ,  A61K47/58 ,  C12N11/08 ,  A61K38/48 ,  C12N9/76

Abstract: Embodiments of the invention provide at least one polymer covalently conjugated to an esterase. The at least one polymer includes a plurality of oxime functional groups.

公开(公告)号：US20200369716A1

公开(公告)日：2020-11-26

申请号：US16635343

申请日：2018-08-01

Applicant: Carnegie Mellon University

Inventor： Hironobu Murata ,  Alan J. Russell

IPC: C07K1/04 ,  C07K1/107 ,  C12N11/087 ,  C08B37/00

Abstract: Materials and methods for generating protein-polymer conjugates on solid supports are provided herein. The methods can include, for example, reversibly immobilizing a protein on a solid support, modifying the protein by adding polymer subunits, and releasing the protein-polymer conjugate from the solid support.

公开(公告)号：US20230079313A1

公开(公告)日：2023-03-16

申请号：US17942952

申请日：2022-09-12

Applicant: Carnegie Mellon University

Inventor： Krzysztof Matyjaszewski ,  Alan Enciso Barros ,  Liye Fu ,  Alan J. Russell

IPC: C08F4/10 ,  C08F2/16 ,  C08K5/1545 ,  C08F4/26 ,  C12P7/00

Abstract: Materials and methods for conducting an atom transfer radical polymerization in the presence of oxygen by interlocking enzymatic activities are provided herein.

公开(公告)号：US20220275137A1

公开(公告)日：2022-09-01

申请号：US17631985

申请日：2020-07-31

Applicant: Carnegie Mellon University

Inventor： Alan J. Russell ,  Stefanie L. Baker

IPC: C08F293/00 ,  C08F4/40

Abstract: Materials and methods for protein purification, and particularly for protein purification by ammonium sulfate precipitation, are provided herein.

公开(公告)号：US20220081329A1

公开(公告)日：2022-03-17

申请号：US17425519

申请日：2020-01-27

Applicant: Carnegie Mellon University

Inventor： Alan J. Russell ,  Philip R. LeDuc ,  Adam Wood

IPC: C02F1/461 ,  C02F1/46 ,  C02F1/469

Abstract: Provided herein are highly porous electrode structures made from natural materials and retaining natural architecture, which are highly permeable and find use in capacitive deionization desalination systems and methods. Also provided herein are methods of making the electrodes and desalination systems, as well as methods of desalinating water.

公开(公告)号：US20210388337A1

公开(公告)日：2021-12-16

申请号：US17284837

申请日：2019-10-11

Applicant: Carnegie Mellon University

Inventor： Hironobu Murata ,  Alan J. Russell

IPC: C12N9/96 ,  C12N11/087 ,  A61K47/62 ,  A61K47/60 ,  A61K47/58

Abstract: This document relates to materials and methods for using controlled radical polymerization (e.g., atom transfer radical polymerization) to generate protein-polymer conjugates in which two or more polymer molecules are attached to individual initiator molecules on the protein.

公开(公告)号：US20190382749A1

公开(公告)日：2019-12-19

申请号：US16556739

申请日：2019-08-30

Applicant: Carnegie Mellon University

Inventor： Alan J. Russell ,  Hironobu Murata ,  Chad S. Cummings ,  Richard R. Koepsel

IPC: C12N9/96 ,  A61K38/46 ,  A61K38/48 ,  C12N9/16 ,  C12N9/76 ,  A61K47/58

Abstract: Embodiments of the invention provide at least one polymer covalently conjugated to an esterase. The at least one polymer includes a plurality of oxime functional groups.

公开(公告)号：US20180051271A1

公开(公告)日：2018-02-22

申请号：US15549852

申请日：2016-02-10

Applicant: CARNEGIE MELLON UNIVERSITY

Inventor： Alan J. Russell ,  Hironobu Murata

IPC: C12N11/08 ,  C12N9/18 ,  C12N9/50 ,  C08L33/10 ,  C08L33/26

CPC classification number: C12N11/08 ,  C08L33/10 ,  C08L33/26 ,  C12N9/00 ,  C12N9/18 ,  C12N9/50

Abstract: An enzyme-polymer conjugate shows increased activity and molecular dissolution in non-aqueous solvents enabling enzyme mediated catalysis in non-aqueous solutions. The inventions described in this specification relate to enzyme-polymer conjugates, organic solutions comprising enzyme-polymer conjugates, methods of dissolving enzyme-polymer conjugates in organic solvents, and methods of using enzyme-polymer conjugates, for example, in catalysis applications. The inventions described in this specification address the problems of insolubility and decreased activity of enzymes in non-aqueous solutions comprising, for example, organic solvents or ionic liquids.

公开(公告)号：US20160244741A1

公开(公告)日：2016-08-25

申请号：US15026093

申请日：2014-10-03

Applicant: CARNEGIE MELLON UNIVERSITY

Inventor： Alan J. Russell ,  Hironobu Murata ,  Chad Cummings ,  Richard R. Koepsel

IPC: C12N9/96 ,  A61K38/48 ,  A61K47/48 ,  C12N9/76 ,  C12N9/16 ,  A61K38/46

CPC classification number: C12N9/96 ,  A61K38/465 ,  A61K38/4826 ,  A61K47/58 ,  C12N9/16 ,  C12N9/6427 ,  C12Y301/01007 ,  C12Y304/21001

Abstract: Embodiments of the invention provide at least one polymer covalently conjugated to an esterase. The at least one polymer includes a plurality of oxime functional groups.

Abstract translation: 本发明的实施方案提供至少一种与酯酶共价缀合的聚合物。 所述至少一种聚合物包括多个肟官能团。