公开(公告)号：US20220389427A1

公开(公告)日：2022-12-08

申请号：US17687473

申请日：2022-03-04

Applicant: Cepheid

Inventor： Sergey G. Lokhov ,  Alexander A. Gall ,  Vera Baraznenok ,  Ekaterina V. Viazovkina ,  Irina Shishkina ,  David H. Persing

IPC: C12N15/115 ,  C12Q1/6853 ,  C12Q1/686

Abstract: The present disclosure relates to aptamers for temperature-dependent reversible inhibition of thermostable polymerase activity in order to improve sensitivity and specificity of various reactions and assays involving hot start polynucleotide synthesis. Methods for use of the aptamers and related compositions and kits are also provided.

公开(公告)号：US10000808B2

公开(公告)日：2018-06-19

申请号：US14445417

申请日：2014-07-29

Applicant: Cepheid

Inventor： Cecilia Svanholm Barrie ,  Olivier Delfour ,  David H. Persing

IPC: C12Q1/68

CPC classification number: C12Q1/6883 ,  C12Q1/6886 ,  C12Q2600/118 ,  C12Q2600/16 ,  C12Q2600/178

Abstract: Methods of detecting cervical dysplasia, such as cervical dysplasia likely to progress to carcinoma in a sample of human cervical cells, are provided. Methods of detecting changes in expression of one or more microRNAs or mRNAs associated with cervical dysplasia or cervical cancer are also provided. Compositions and kits are also provided.

公开(公告)号：US20150176057A1

公开(公告)日：2015-06-25

申请号：US14553622

申请日：2014-11-25

Applicant: Cepheid

Inventor： Joseph H. Smith ,  David H. Persing ,  Alan Wortman ,  Ronald Chang ,  David Swenson

IPC: C12Q1/68 ,  B01L3/00 ,  B01L7/00

CPC classification number: C12Q1/686 ,  B01L3/502738 ,  B01L7/52 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2400/0644 ,  C12Q1/6848 ,  C12Q1/6851 ,  C12Q1/689 ,  C12Q2565/629 ,  C12Q2547/101 ,  C12Q2537/149 ,  C12Q2537/143 ,  C12Q2565/101 ,  C12Q2527/107

Abstract: The invention provides methods and apparatus for carrying out multiple amplification reactions in a single reaction chamber by successive cycles of loading reaction mixture, amplifying, and removing spent reaction mixture in a fluidly closed reaction system. In particular, the present invention allows amplification of a plurality of target polynucleotides from a single sample by carrying out under closed-loop control successive amplifications of different target polynucleotides from different portions of the sample.

Abstract translation: 本发明提供了在单个反应室中进行多次扩增反应的方法和装置，其通过连续循环的负载反应混合物，在流体封闭的反应体系中放大和除去废反应混合物。 特别地，本发明允许通过在闭环控制下从样品的不同部分连续扩增不同靶多核苷酸而从单个样品扩增多个靶多核苷酸。