公开(公告)号：US20180187243A1

公开(公告)日：2018-07-05

申请号：US15849125

申请日：2017-12-20

申请人： Cepheid

发明人： Joseph H. Smith ,  David H. Persing ,  Alan Wortman ,  Ronald Chang ,  David Swenson

IPC分类号： C12Q1/686 ,  B01L7/00 ,  C12Q1/689 ,  C12Q1/6848 ,  B01L3/00 ,  C12Q1/6851

CPC分类号： C12Q1/686 ,  B01L3/502738 ,  B01L7/52 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2400/0644 ,  C12Q1/6848 ,  C12Q1/6851 ,  C12Q1/689 ,  C12Q2565/629 ,  C12Q2547/101 ,  C12Q2537/149 ,  C12Q2537/143 ,  C12Q2565/101 ,  C12Q2527/107

摘要： The invention provides methods and apparatus for carrying out multiple amplification reactions in a single reaction chamber by successive cycles of loading reaction mixture, amplifying, and removing spent reaction mixture in a fluidly closed reaction system. In particular, the present invention allows amplification of a plurality of target polynucleotides from a single sample by carrying out under closed-loop control successive amplifications of different target polynucleotides from different portions of the sample.

公开(公告)号：US12123052B2

公开(公告)日：2024-10-22

申请号：US17130394

申请日：2020-12-22

申请人： Cepheid

发明人： Joseph H. Smith ,  David H. Persing ,  Alan Wortman ,  Ronald Chang ,  David Swenson

IPC分类号： C12Q1/686 ,  B01L3/00 ,  B01L7/00 ,  C12Q1/6848 ,  C12Q1/6851 ,  C12Q1/689

CPC分类号： C12Q1/686 ,  B01L3/502738 ,  B01L7/52 ,  C12Q1/6848 ,  C12Q1/6851 ,  C12Q1/689 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2400/0644 ,  C12Q1/6848 ,  C12Q2565/629 ,  C12Q2547/101 ,  C12Q2537/149 ,  C12Q1/6848 ,  C12Q2565/629 ,  C12Q2565/101 ,  C12Q2527/107 ,  C12Q1/6851 ,  C12Q2565/629 ,  C12Q2537/149 ,  C12Q2537/143 ,  C12Q1/686 ,  C12Q2565/629 ,  C12Q2537/149 ,  C12Q2537/143

摘要： The invention provides methods and apparatus for carrying out multiple amplification reactions in a single reaction chamber by successive cycles of loading reaction mixture, amplifying, and removing spent reaction mixture in a fluidly closed reaction system. In particular, the present invention allows amplification of a plurality of target polynucleotides from a single sample by carrying out under closed-loop control successive amplifications of different target polynucleotides from different portions of the sample.

公开(公告)号：US20210285025A1

公开(公告)日：2021-09-16

申请号：US17130394

申请日：2020-12-22

申请人： Cepheid

发明人： Joseph H. Smith ,  David H. Persing ,  Alan Wortman ,  Ronald Chang ,  David Swenson

IPC分类号： C12Q1/686 ,  C12Q1/6851 ,  C12Q1/6848 ,  B01L3/00 ,  B01L7/00 ,  C12Q1/689

摘要： The invention provides methods and apparatus for carrying out multiple amplification reactions in a single reaction chamber by successive cycles of loading reaction mixture, amplifying, and removing spent reaction mixture in a fluidly closed reaction system. In particular, the present invention allows amplification of a plurality of target polynucleotides from a single sample by carrying out under closed-loop control successive amplifications of different target polynucleotides from different portions of the sample.

公开(公告)号：US10907202B2

公开(公告)日：2021-02-02

申请号：US15849125

申请日：2017-12-20

申请人： Cepheid

发明人： Joseph H. Smith ,  David H. Persing ,  Alan Wortman ,  Ronald Chang ,  David Swenson

IPC分类号： C12Q1/686 ,  C12Q1/6851 ,  C12Q1/6848 ,  B01L3/00 ,  B01L7/00 ,  C12Q1/689

摘要： The invention provides methods and apparatus for carrying out multiple amplification reactions in a single reaction chamber by successive cycles of loading reaction mixture, amplifying, and removing spent reaction mixture in a fluidly closed reaction system. In particular, the present invention allows amplification of a plurality of target polynucleotides from a single sample by carrying out under closed-loop control successive amplifications of different target polynucleotides from different portions of the sample.

公开(公告)号：US20150376711A1

公开(公告)日：2015-12-31

申请号：US14721098

申请日：2015-05-26

申请人： Cepheid

发明人： Bernard Michot ,  Olivier Delfour ,  David H. Persing

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6886 ,  C12Q2600/158 ,  C12Q2600/178

摘要： Methods of lung cancer in a sample from a patient are provided. Methods of detecting changes in expression of one or more target RNAs associated with lung cancer are also provided. Compositions and kits are also provided.

摘要翻译： 提供了来自患者的样品中的肺癌的方法。 还提供了检测与肺癌相关的一种或多种靶RNA的表达变化的方法。 还提供了组合和成套工具。

公开(公告)号：US11299739B2

公开(公告)日：2022-04-12

申请号：US16305858

申请日：2017-06-06

申请人： Cepheid

发明人： Sergey G. Lokhov ,  Alexander A. Gall ,  Vera Baraznenok ,  Ekaterina V. Viazovkina ,  Irina Shishkina ,  David H. Persing

IPC分类号： C12Q1/6853 ,  C12Q1/686 ,  C12N15/115

摘要： The present disclosure relates to aptamers for temperature-dependent reversible inhibition of thermostable polymerase activity in order to improve sensitivity and specificity of various reactions and assays involving hot start polynucleotide synthesis. Methods for use of the aptamers and related compositions and kits are also provided.

公开(公告)号：US09873909B2

公开(公告)日：2018-01-23

申请号：US14553622

申请日：2014-11-25

申请人： Cepheid

发明人： Joseph H. Smith ,  David H. Persing ,  Alan Wortman ,  Ronald Chang ,  David Swenson

IPC分类号： C12P19/34 ,  C12Q1/68 ,  B01L3/00 ,  B01L7/00

CPC分类号： C12Q1/686 ,  B01L3/502738 ,  B01L7/52 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2400/0644 ,  C12Q1/6848 ,  C12Q1/6851 ,  C12Q1/689 ,  C12Q2565/629 ,  C12Q2547/101 ,  C12Q2537/149 ,  C12Q2537/143 ,  C12Q2565/101 ,  C12Q2527/107

摘要： The invention provides methods and apparatus for carrying out multiple amplification reactions in a single reaction chamber by successive cycles of loading reaction mixture, amplifying, and removing spent reaction mixture in a fluidly closed reaction system. In particular, the present invention allows amplification of a plurality of target polynucleotides from a single sample by carrying out under closed-loop control successive amplifications of different target polynucleotides from different portions of the sample.

公开(公告)号：US20140200153A1

公开(公告)日：2014-07-17

申请号：US14079741

申请日：2013-11-14

申请人： Cepheid

发明人： Bernard Michot ,  Olivier Delfour ,  David H. Persing

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6886 ,  C12Q2600/158 ,  C12Q2600/178

摘要： Methods of lung cancer in a sample from a patient are provided. Methods of detecting changes in expression of one or more target RNAs associated with lung cancer are also provided. Compositions and kits are also provided.

摘要翻译： 提供了来自患者的样品中的肺癌的方法。 还提供了检测与肺癌相关的一种或多种靶RNA的表达变化的方法。 还提供了组合和成套工具。

公开(公告)号：US20190316132A1

公开(公告)日：2019-10-17

申请号：US16305858

申请日：2017-06-06

申请人： Cepheid

发明人： Sergey G. Lokhov ,  Alexander A. Gall ,  Vera Baraznenok ,  Ekaterina V. Viazovkina ,  Irina Shishkina ,  David H. Persing

IPC分类号： C12N15/115 ,  C12Q1/6853 ,  C12Q1/686

摘要： The present disclosure relates to aptamers for temperature-dependent reversible inhibition of thermostable polymerase activity in order to improve sensitivity and specificity of various reactions and assays involving hot start polynucleotide synthesis. Methods for use of the aptamers and related compositions and kits are also provided.

公开(公告)号：US20150051103A1

公开(公告)日：2015-02-19

申请号：US14445417

申请日：2014-07-29

申请人： Cepheid

发明人： Cecilia Svanholm Barrie ,  Olivier Delfour ,  David H. Persing

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6883 ,  C12Q1/6886 ,  C12Q2600/118 ,  C12Q2600/16 ,  C12Q2600/178

摘要： Methods of detecting cervical dysplasia, such as cervical dysplasia likely to progress to carcinoma in a sample of human cervical cells, are provided. Methods of detecting changes in expression of one or more microRNAs or mRNAs associated with cervical dysplasia or cervical cancer are also provided. Compositions and kits are also provided.

摘要翻译： 提供宫颈发育不良的检测方法，例如可能在人宫颈细胞样本中进展为宫颈发育异常的方法。 还提供了检测与子宫颈发育不良或宫颈癌相关的一种或多种微小RNA或mRNA的表达变化的方法。 还提供了组合和成套工具。