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    Methods and apparatus for point-of-care nucleic acid amplification and detection
    3.
    发明授权
    Methods and apparatus for point-of-care nucleic acid amplification and detection 有权
    点对点核酸扩增和检测的方法和装置

    公开(公告)号：US09469871B2

    公开(公告)日：2016-10-18

    申请号：US14262683

    申请日：2014-04-25

    申请人： CORPOROS INC.

    发明人： Jane P. Bearinger ,  Scott Castanon ,  Kenneth J. Michlitsch

    IPC分类号： C12Q1/68 ,  B01L3/00 ,  B01L7/00

    CPC分类号： C12Q1/6844 ,  B01L3/502715 ,  B01L3/502723 ,  B01L3/502738 ,  B01L7/00 ,  B01L2300/0861 ,  B01L2300/18 ,  B01L2400/06 ,  C12Q1/6825 ,  C12Q1/6846 ,  C12Q2547/101 ,  C12Q2563/173 ,  C12Q2565/607 ,  C12Q2527/101 ,  C12Q2563/107 ,  C12Q2565/629

    摘要： Methods and apparatus are provided for point-of-care nucleic acid amplification and detection. One embodiment of the invention comprises a fully integrated, sample-to-answer molecular diagnostic instrument that optionally may be used in a multiplexed fashion to detect multiple target nucleic acid sequences of interest and that optionally may be configured for disposal after one-time use. The instrument preferable utilizes an isothermal nucleic acid amplification technique, such as loop-mediated isothermal amplification (LAMP), to reduce the instrumentation requirements associated with nucleic acid amplification. Detection of target amplification may be achieved, for example, via detection of a color shift or fluorescence in a dye added to the amplification reaction. Such detection may be performed visually by an operator or may be achieved utilizing an imaging technique, e.g., spectrophotometric imaging.

    摘要翻译： 提供了用于点对点核酸扩增和检测的方法和装置。 本发明的一个实施方案包括完全整合的样品至答复分子诊断仪器，其可任选地以多路复用方式使用以检测目标的多个靶核酸序列，并且可任选地配置为在一次使用后进行处置。 该仪器优选使用等温核酸扩增技术，例如环介导的等温扩增（LAMP），以减少与核酸扩增相关的仪器要求。 靶扩增的检测可以例如通过检测加入到扩增反应中的染料中的色移或荧光来实现。 这种检测可以由操作者视觉执行，或者可以使用成像技术（例如分光光度成像）来实现。

    SYSTEM AND METHOD FOR LASER LYSIS
    5.
    发明申请
    SYSTEM AND METHOD FOR LASER LYSIS 审中-公开
    激光雷达系统和方法

    公开(公告)号：US20160237476A1

    公开(公告)日：2016-08-18

    申请号：US15135150

    申请日：2016-04-21

    申请人： ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

    发明人： Deirdre Meldrum ,  Shih-Hui (Joseph) Chao ,  Thai Tran ,  Laimonas Kelbauskas ,  Jeff Houkal ,  Andrew Hatch ,  Weimin Gao ,  David Richardson

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6806 ,  B01L3/0293 ,  B01L3/502761 ,  B01L2200/0668 ,  B01L2300/0816 ,  B01L2300/0829 ,  B01L2300/0864 ,  C12M47/06 ,  C12Q1/6841 ,  C12Q1/6848 ,  C12Q1/686 ,  G01N1/286 ,  G01N2001/2886 ,  C12Q2547/101

    摘要： Systems and methods for in situ laser lysis for analysis of biological tissue (live, fixed, frozen or otherwise preserved) at single cell resolution in 3D. For example, a system and method for lysing individual cells in situ, including the steps of capturing a tissue sample comprising a cellular content, subjecting the tissue sample to a stream of continuous fluid flow, lysing a selected area of the tissue sample with a laser, thereby releasing at least a portion of the cellular content from the tissue sample, recovering at least one target molecule from the cellular content in the stream, and processing at least one target molecule is provided. The system collects cellular contents, performs highly multiplexed (RT-qPCR or RNA-seq), and sequentially (cell-by-cell) reconstructs a 3D spatial map of mRNA expression of the tissue with a large number of genes. A 3D spatial map of the DNA, RNA, and/or proteins can be generated for each cell in the tissue.

    摘要翻译： 用于原位激光裂解的系统和方法，用于分析3D中单细胞分辨率的生物组织（活，固定，冷冻或以其他方式保存）。 例如，用于原位裂解单个细胞的系统和方法，包括以下步骤：捕获包含细胞内容物的组织样品，使组织样品经受连续流体流的流动，用激光裂解组织样品的选定区域 从而从组织样品释放至少一部分细胞含量，从流中的细胞含量回收至少一种靶分子，并且提供至少一种靶分子的加工。 系统收集细胞内容，进行高度多重化（RT-qPCR或RNA-seq），并依次（逐个细胞）重构具有大量基因的组织的mRNA表达的3D空间图。 可以为组织中的每个细胞产生DNA，RNA和/或蛋白质的3D空间图。

    AUTOMATED ISOLATION AND CHEMICAL REACTION(S) OF NUCLEIC ACIDS
    7.
    发明申请
    AUTOMATED ISOLATION AND CHEMICAL REACTION(S) OF NUCLEIC ACIDS 审中-公开
    自动分离和化学反应的核酸

    公开(公告)号：US20160108459A1

    公开(公告)日：2016-04-21

    申请号：US14517759

    申请日：2014-10-17

    申请人： BioChain Institute Inc.

    发明人： Xiaoliang HAN ,  James Jianming WANG ,  Tong LU

    IPC分类号： C12Q1/68 ,  B01L7/00

    CPC分类号： C12Q1/6806 ,  B01L3/50851 ,  G01N35/0098 ,  G01N2035/00564 ,  C12Q2523/125 ,  C12Q2547/101

    摘要： The present teachings relate to methods, kits and devices for performing automated sequential nucleic acid isolation and conversion/purification in a single closed system. In various embodiments, the present teaching enable a user to (i) load a device with test samples, reagents and consumables; (ii) select or program the device for the desired nucleic acid isolation and subsequent chemical treatment and/or conversion reaction(s) without further user intervention; and recovering the isolated and treated and/or converted nucleic acid at the conclusion of the program once the device is activated.

    摘要翻译： 本教导涉及用于在单个封闭系统中执行自动化顺序核酸分离和转化/纯化的方法，试剂盒和装置。 在各种实施例中，本教导使得用户能够（i）加载具有测试样品，试剂和消耗品的装置; （ii）选择或编程器件以进行所需的核酸分离和随后的化学处理和/或转化反应，而无需进一步的用户干预; 并且一旦该装置被激活，就在程序结束时回收分离的和处理的和/或转化的核酸。