公开(公告)号：US12123052B2

公开(公告)日：2024-10-22

申请号：US17130394

申请日：2020-12-22

Applicant: Cepheid

Inventor： Joseph H. Smith ,  David H. Persing ,  Alan Wortman ,  Ronald Chang ,  David Swenson

IPC: C12Q1/686 ,  B01L3/00 ,  B01L7/00 ,  C12Q1/6848 ,  C12Q1/6851 ,  C12Q1/689

CPC classification number: C12Q1/686 ,  B01L3/502738 ,  B01L7/52 ,  C12Q1/6848 ,  C12Q1/6851 ,  C12Q1/689 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2400/0644 ,  C12Q1/6848 ,  C12Q2565/629 ,  C12Q2547/101 ,  C12Q2537/149 ,  C12Q1/6848 ,  C12Q2565/629 ,  C12Q2565/101 ,  C12Q2527/107 ,  C12Q1/6851 ,  C12Q2565/629 ,  C12Q2537/149 ,  C12Q2537/143 ,  C12Q1/686 ,  C12Q2565/629 ,  C12Q2537/149 ,  C12Q2537/143

Abstract: The invention provides methods and apparatus for carrying out multiple amplification reactions in a single reaction chamber by successive cycles of loading reaction mixture, amplifying, and removing spent reaction mixture in a fluidly closed reaction system. In particular, the present invention allows amplification of a plurality of target polynucleotides from a single sample by carrying out under closed-loop control successive amplifications of different target polynucleotides from different portions of the sample.

公开(公告)号：US20210285025A1

公开(公告)日：2021-09-16

申请号：US17130394

申请日：2020-12-22

Applicant: Cepheid

Inventor： Joseph H. Smith ,  David H. Persing ,  Alan Wortman ,  Ronald Chang ,  David Swenson

IPC: C12Q1/686 ,  C12Q1/6851 ,  C12Q1/6848 ,  B01L3/00 ,  B01L7/00 ,  C12Q1/689

Abstract: The invention provides methods and apparatus for carrying out multiple amplification reactions in a single reaction chamber by successive cycles of loading reaction mixture, amplifying, and removing spent reaction mixture in a fluidly closed reaction system. In particular, the present invention allows amplification of a plurality of target polynucleotides from a single sample by carrying out under closed-loop control successive amplifications of different target polynucleotides from different portions of the sample.

公开(公告)号：US10907202B2

公开(公告)日：2021-02-02

申请号：US15849125

申请日：2017-12-20

Applicant: Cepheid

Inventor： Joseph H. Smith ,  David H. Persing ,  Alan Wortman ,  Ronald Chang ,  David Swenson

IPC: C12Q1/686 ,  C12Q1/6851 ,  C12Q1/6848 ,  B01L3/00 ,  B01L7/00 ,  C12Q1/689

Abstract: The invention provides methods and apparatus for carrying out multiple amplification reactions in a single reaction chamber by successive cycles of loading reaction mixture, amplifying, and removing spent reaction mixture in a fluidly closed reaction system. In particular, the present invention allows amplification of a plurality of target polynucleotides from a single sample by carrying out under closed-loop control successive amplifications of different target polynucleotides from different portions of the sample.

公开(公告)号：US09873909B2

公开(公告)日：2018-01-23

申请号：US14553622

申请日：2014-11-25

Applicant: Cepheid

Inventor： Joseph H. Smith ,  David H. Persing ,  Alan Wortman ,  Ronald Chang ,  David Swenson

IPC: C12P19/34 ,  C12Q1/68 ,  B01L3/00 ,  B01L7/00

CPC classification number: C12Q1/686 ,  B01L3/502738 ,  B01L7/52 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2400/0644 ,  C12Q1/6848 ,  C12Q1/6851 ,  C12Q1/689 ,  C12Q2565/629 ,  C12Q2547/101 ,  C12Q2537/149 ,  C12Q2537/143 ,  C12Q2565/101 ,  C12Q2527/107

Abstract: The invention provides methods and apparatus for carrying out multiple amplification reactions in a single reaction chamber by successive cycles of loading reaction mixture, amplifying, and removing spent reaction mixture in a fluidly closed reaction system. In particular, the present invention allows amplification of a plurality of target polynucleotides from a single sample by carrying out under closed-loop control successive amplifications of different target polynucleotides from different portions of the sample.

公开(公告)号：US20180187243A1

公开(公告)日：2018-07-05

申请号：US15849125

申请日：2017-12-20

Applicant: Cepheid

Inventor： Joseph H. Smith ,  David H. Persing ,  Alan Wortman ,  Ronald Chang ,  David Swenson

IPC: C12Q1/686 ,  B01L7/00 ,  C12Q1/689 ,  C12Q1/6848 ,  B01L3/00 ,  C12Q1/6851

CPC classification number: C12Q1/686 ,  B01L3/502738 ,  B01L7/52 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2400/0644 ,  C12Q1/6848 ,  C12Q1/6851 ,  C12Q1/689 ,  C12Q2565/629 ,  C12Q2547/101 ,  C12Q2537/149 ,  C12Q2537/143 ,  C12Q2565/101 ,  C12Q2527/107

Abstract: The invention provides methods and apparatus for carrying out multiple amplification reactions in a single reaction chamber by successive cycles of loading reaction mixture, amplifying, and removing spent reaction mixture in a fluidly closed reaction system. In particular, the present invention allows amplification of a plurality of target polynucleotides from a single sample by carrying out under closed-loop control successive amplifications of different target polynucleotides from different portions of the sample.

公开(公告)号：US20150176057A1

公开(公告)日：2015-06-25

申请号：US14553622

申请日：2014-11-25

Applicant: Cepheid

Inventor： Joseph H. Smith ,  David H. Persing ,  Alan Wortman ,  Ronald Chang ,  David Swenson

IPC: C12Q1/68 ,  B01L3/00 ,  B01L7/00

CPC classification number: C12Q1/686 ,  B01L3/502738 ,  B01L7/52 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2400/0644 ,  C12Q1/6848 ,  C12Q1/6851 ,  C12Q1/689 ,  C12Q2565/629 ,  C12Q2547/101 ,  C12Q2537/149 ,  C12Q2537/143 ,  C12Q2565/101 ,  C12Q2527/107

Abstract: The invention provides methods and apparatus for carrying out multiple amplification reactions in a single reaction chamber by successive cycles of loading reaction mixture, amplifying, and removing spent reaction mixture in a fluidly closed reaction system. In particular, the present invention allows amplification of a plurality of target polynucleotides from a single sample by carrying out under closed-loop control successive amplifications of different target polynucleotides from different portions of the sample.

Abstract translation: 本发明提供了在单个反应室中进行多次扩增反应的方法和装置，其通过连续循环的负载反应混合物，在流体封闭的反应体系中放大和除去废反应混合物。 特别地，本发明允许通过在闭环控制下从样品的不同部分连续扩增不同靶多核苷酸而从单个样品扩增多个靶多核苷酸。