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    EXPONENTIAL BASE-3 AND GREATER NUCLEIC ACID AMPLIFICATION WITH REDUCED AMPLIFICATION TIME
    11.
    发明申请
    EXPONENTIAL BASE-3 AND GREATER NUCLEIC ACID AMPLIFICATION WITH REDUCED AMPLIFICATION TIME 有权

    公开(公告)号：US20230096557A1

    公开(公告)日：2023-03-30

    申请号：US17737880

    申请日：2022-05-05

    Applicant: Cepheid

    Inventor： Russell Higuchi

    IPC: C12N15/11 ,  C12Q1/6876 ,  C12P19/34

    Abstract: Described herein are methods and compositions that provide highly efficient nucleic acid amplification. In some embodiments, this allows a 3-fold or greater increase of amplification product for each amplification cycle and therefore increased sensitivity and speed over conventional PCR. Modified bases can be employed in primers to provide this base-3 or greater amplification with satisfactory PCR cycle times, which are improved, as compared to those observed in the absence of modified bases.

    INTEGRATED PURIFICATION AND MEASUREMENT OF DNA METHYLATION AND CO-MEASUREMENT OF MUTATIONS AND/OR MRNA EXPRESSION LEVELS IN AN AUTOMATED REACTION CARTRIDGE
    12.
    发明申请
    INTEGRATED PURIFICATION AND MEASUREMENT OF DNA METHYLATION AND CO-MEASUREMENT OF MUTATIONS AND/OR MRNA EXPRESSION LEVELS IN AN AUTOMATED REACTION CARTRIDGE 有权

    公开(公告)号：US20220226809A1

    公开(公告)日：2022-07-21

    申请号：US17586645

    申请日：2022-01-27

    Applicant: Cepheid

    Inventor： Edwin Wei-Lung Lai ,  Andrew Kohlway ,  Reuel Van Atta ,  Russell Higuchi ,  Alexander A. Gall ,  Kriszten Kocmond

    IPC: B01L3/00 ,  B01L7/00 ,  C12Q1/6827 ,  C12N15/10 ,  C12Q1/6853 ,  C12Q1/686

    Abstract: Methods of determining methylation of DNA are provided. In one illustrative, but non-limiting embodiment the method comprises i) contacting a biological sample comprising a nucleic acid to a first matrix material comprising a first column or filter where said matrix material binds and/or filters nucleic acids in said sample and thereby purifies the DNA; ii) eluting the bound DNA from the first matrix material and denaturing the DNA to produce eluted denatured DNA; iii) heating the eluted DNA in the presence of bisulfite ions to produce a deaminated nucleic acid; iv) contacting said deaminated nucleic acid to a second matrix material comprising a second column to bind said deaminated nucleic acid to said second matrix material; v) desulphonating the bound deaminated nucleic acid and/or simultaneously eluting and desulphonating the nucleic acid by contacting the deaminated nucleic acid with an alkaline solution to produce a bisulfite converted nucleic acid; vi) eluting said bisulfite converted nucleic acid from said second matrix material; and vii) performing methylation specific PCR and/or nucleic acid sequencing, and/or high resolution melting analysis (HRM) on said bisulfite-converted nucleic acid to determine the methylation of said nucleic acid, wherein at least steps iv) through vi) are performed in a single reaction cartridge.

    Methods of Detecting Bladder Cancer
    13.
    发明申请
    Methods of Detecting Bladder Cancer 审中-公开

    公开(公告)号：US20190382847A1

    公开(公告)日：2019-12-19

    申请号：US16411485

    申请日：2019-05-14

    Applicant: Cepheid

    Inventor： Leena McCann ,  Stacey Ellen Wallace ,  Edwin Wei-Lung Lai ,  Russell Higuchi

    IPC: C12Q1/6886

    Abstract: Compositions and methods for detecting bladder cancer are provided. In some embodiments, methods of detecting low grade bladder cancer are provided. In some embodiments, methods of monitoring recurrence of bladder cancer are provided. In some embodiments, the methods comprise detecting androgen receptor (AR) and/or uroplakin 1B (UPK1B).

    Integrated purification and measurement of DNA methylation and co-measurement of mutations and/or MRNA expression levels in an automated reaction cartridge
    16.
    发明授权
    Integrated purification and measurement of DNA methylation and co-measurement of mutations and/or MRNA expression levels in an automated reaction cartridge 有权

    公开(公告)号：US11603555B2

    公开(公告)日：2023-03-14

    申请号：US15182394

    申请日：2016-06-14

    Applicant: Cepheid

    Inventor： Edwin W. Lai ,  Andrew Kohlway ,  Reuel Vanatta ,  Russell Higuchi ,  Alexander Gall ,  Kriszten Kocmond

    IPC: C12Q1/6806 ,  C12Q1/6827 ,  C12Q1/6886 ,  C12N15/10 ,  B01L7/00

    Abstract: In various embodiments methods of determining methylation of DNA are provided. In one illustrative, but non-limiting embodiment the method comprises i) contacting a biological sample comprising a nucleic acid to a first matrix material comprising a first column or filter where said matrix material binds and/or filters nucleic acids in said sample and thereby purifies the DNA; ii) eluting the bound DNA from the first matrix material and denaturing the DNA to produce eluted denatured DNA; iii) heating the eluted DNA in the presence of bisulfite ions to produce a deaminated nucleic acid; iv) contacting said deaminated nucleic acid to a second matrix material comprising a second column to bind said deaminated nucleic acid to said second matrix material; v) desulfonating the bound deaminated nucleic acid and/or simultaneously eluting and desulfonating the nucleic acid by contacting the deaminated nucleic acid with an alkaline solution to produce a bisulfite converted nucleic acid; vi) eluting said bisulfite converted nucleic acid from said second matrix material; and vii) performing methylation specific PCR and/or nucleic acid sequencing, and/or high resolution melting analysis (HRM) on said bisulfite-converted nucleic acid to determine the methylation of said nucleic acid, wherein at least steps iv) through vi) are performed in a single reaction cartridge.

    METHODS OF DETECTING BLADDER CANCER
    17.
    发明申请
    METHODS OF DETECTING BLADDER CANCER 审中-公开
    检测乳腺癌的方法

    公开(公告)号：US20150133325A1

    公开(公告)日：2015-05-14

    申请号：US14394352

    申请日：2013-04-19

    Applicant: Cepheid

    Inventor： Russell Higuchi ,  Stacey Ellen Wallace ,  Edwin Wei-Lung Lai

    IPC: C12Q1/68

    CPC classification number: C12Q1/6886 ,  C12Q2600/158 ,  C12Q2600/16 ,  C12Q2600/166

    Abstract: Compositions and methods for detecting bladder cancer are provided. In some embodiments, methods of monitoring recurrence of bladder cancer are provided. In some embodiments, the methods comprise detecting a set of markers consisting of CRH, IGF2, KRT20, and ANXA10.

    Abstract translation: 提供了检测膀胱癌的组合物和方法。 在一些实施方案中，提供了监测膀胱癌复发的方法。 在一些实施方案中，所述方法包括检测由CRH，IGF2，KRT20和ANXA10组成的一组标志物。

    METHODS OF DETECTING BLADDER CANCER
    18.
    发明申请
    METHODS OF DETECTING BLADDER CANCER 审中-公开
    检测乳腺癌的方法

    公开(公告)号：US20140309127A1

    公开(公告)日：2014-10-16

    申请号：US14199292

    申请日：2014-03-06

    Applicant: Cepheid

    Inventor： Leena McCann ,  Stacey Ellen Wallace ,  Edwin Wei-Lung Lai ,  Russell Higuchi

    IPC: C12Q1/68

    CPC classification number: C12Q1/6886 ,  C12Q2600/106 ,  C12Q2600/158

    Abstract: Compositions and methods for detecting bladder cancer are provided. In some embodiments, methods of detecting low grade bladder cancer are provided. In some embodiments, methods of monitoring recurrence of bladder cancer are provided. In some embodiments, the methods comprise detecting androgen receptor (AR) and/or uroplakin 1B (UPK1B).

    Abstract translation: 提供了检测膀胱癌的组合物和方法。 在一些实施方案中，提供了检测低级膀胱癌的方法。 在一些实施方案中，提供了监测膀胱癌复发的方法。 在一些实施方案中，所述方法包括检测雄激素受体（AR）和/或乌普拉汀1B（UPK1B）。

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