公开(公告)号：US06686195B1

公开(公告)日：2004-02-03

申请号：US09937992

申请日：2001-12-13

申请人： Bruno Colin ,  Philippe Cleuziat ,  Patrick Broyer ,  Claude Mabilat ,  Sandra Incardona

发明人： Bruno Colin ,  Philippe Cleuziat ,  Patrick Broyer ,  Claude Mabilat ,  Sandra Incardona

IPC分类号： C12M133

CPC分类号： B01J19/10 ,  B06B3/00 ,  C12M45/02

摘要： An apparatus including at least one sonotrode (2) designed to generate ultrasound of variable power in at least one biological sample (5) containing cells to be lysed, the sample (5) being contained in at least one receptacle (4 or 10) such that the sonotrode (2) is in direct contact with the receptacle(s) (4). Also disclosed is a method for using ultrasound to lyse a biological sample (5) contained in a receptacle (4), which includes placing the receptacle (4) in direct contact with the sonotrode (2), and activating the sonotrode (2) for long enough to lyse the cells in the sample (5) but preserve the DNA and/or RNA molecules released for subsequent operations, e.g. amplification. The invention is particularly applicable in the discipline of molecular biology.

摘要翻译： 一种装置，包括至少一个超声波发生器（2），其被设计成在至少一个含有待裂解细胞的生物样品（5）中产生可变功率的超声波，所述样品（5）包含在至少一个容器（4或10）中， 超声波发生器（2）与容器（4）直接接触。 还公开了一种使用超声波来溶解包含在容器（4）中的生物样品（5）的方法，其包括将容器（4）放置在与超声焊极（2）直接接触的位置，并且激活超声焊极（2）以用于 足以裂解样品（5）中的细胞，但保留释放用于后续操作的DNA和/或RNA分子，例如 放大。 本发明特别适用于分子生物学学科。

公开(公告)号：US5786147A

公开(公告)日：1998-07-28

申请号：US717526

申请日：1996-09-17

申请人： Claude Mabilat ,  Raoult Didier

发明人： Claude Mabilat ,  Raoult Didier

IPC分类号： C12Q1/68 ,  C07H21/02

CPC分类号： C12Q1/689

摘要： The invention relates to a single-stranded oligonucleotide chosen from oligonucleotides having a sequence of at least 12 consecutive nucleotide units which is included in one of the sequences SEQ ID NO: 1 to SEQ ID NO: 52, and from the oligonucleotides complementary to these oligonucleotides, and to the applications of this oligonucleotide for detecting enterobacteria.

摘要翻译： 本发明涉及选自具有至少12个连续核苷酸单元的序列的寡核苷酸的单链寡核苷酸，其包含在SEQ ID NO：1至SEQ ID NO：52中的一个序列中，以及与这些寡核苷酸互补的寡核苷酸 ，以及该寡核苷酸检测肠杆菌的应用。

公开(公告)号：US20050069890A1

公开(公告)日：2005-03-31

申请号：US10500646

申请日：2003-01-10

申请人： Claude Mabilat ,  Sabine Desvarenne ,  Odile Babola ,  Bruno Lacroix ,  Natalia Bello Pigem

发明人： Claude Mabilat ,  Sabine Desvarenne ,  Odile Babola ,  Bruno Lacroix ,  Natalia Bello Pigem

IPC分类号： C12N15/09 ,  C12M1/00 ,  C12Q1/68 ,  C12Q1/6876

CPC分类号： C12Q1/6876 ,  C12Q2600/16

摘要： Disclosed is a method for detecting or identifying the original animal species in a sample likely to contain an ingredient obtained at least from said species. The inventive method is characterized by the following steps: a) a nuclear fraction is obtained from said sample; b) at least one reactant which is specific to the animal species is provided and selected among a group formed by: - the reference sequences SEQ ID numbers 1 to 232, 242 to 261; - the sequences complementing each of the sequences SED ID numbers 1 to 232, 242 to 261, respectively; - the sequences homologous to each of the sequences SEQ ID numbers 1 to 232, 242 to 261 and sequences complementing each of the sequences SED ID numbers 1 to 232, 242 to 261, respectively; c) the nuclear fraction and said reactant are reacted with each other; and d) any signal or information resulting from the specific reaction between said reactant and the nuclear fraction is detected, whereby it can be established if said sample contains said original animal species.

摘要翻译： 公开了一种用于检测或鉴定可能含有至少从所述物种获得的成分的样品中的原始动物物种的方法。 本发明的方法的特征在于以下步骤：a）从所述样品获得核级分; b）至少一种对动物物种具有特异性的反应物，由选自下组形成的基团： - 参考序列SEQ ID号1至232,242至261; - 分别与每个序列SED ID号1至232,242至261互补的序列; - 与SEQ ID号1至232,242至261中的每一个序列同源的序列和分别与每个序列SED ID编号1至232,242至261互补的序列; c）核部分和所述反应物彼此反应; 并且d）检测由所述反应物和核部分之间的特异性反应产生的任何信号或信息，由此如果所述样品包含所述原始动物物种可以确定。

公开(公告)号：US5770362A

公开(公告)日：1998-06-23

申请号：US485180

申请日：1995-06-07

申请人： Jean-Pierre Claverys ,  Claude Mabilat

发明人： Jean-Pierre Claverys ,  Claude Mabilat

IPC分类号： C12Q1/68

CPC分类号： C12Q1/689

摘要： The invention relates to a fragment of the genomic DNA of Strepeococcus pneumoniae, a probe capable of specifically hybridizing with the genomic DNA of Streptococcus pneumoniae, a specific primer for the amplification, by polymerization, of the genomic DNA, a reagent and a method which are used with the probe and, optionally, the primer, for specifically detecting Streptococcus pneumoniae in a biological sample. The probe of the invention is a nucleotide sequence having at least 70% homology with at least a portion of a consensus sequence of the genomic DNA of Streptococcus pneumoniae, this consensus sequence being chosen from the nucleotide sequences SEQ ID NO 2, SEQ ID NO 3, SEQ ID NO 4, which are identified in the description, and their respective complementary sequences.

摘要翻译： 本发明涉及肺炎链球菌的基因组DNA的片段，能够与肺炎链球菌的基因组DNA特异性杂交的探针，用于扩增，聚合的特异性引物，基因组DNA，试剂和方法 与探针一起使用，以及任选的引物用于特异性检测生物样品中的肺炎链球菌。 本发明的探针是与肺炎链球菌的基因组DNA的共有序列的至少一部分具有至少70％同源性的核苷酸序列，该共有序列选自核苷酸序列SEQ ID NO：2，SEQ ID NO： ，SEQ ID NO 4，以及它们各自的互补序列。

公开(公告)号：US5695926A

公开(公告)日：1997-12-09

申请号：US255892

申请日：1994-06-07

申请人： Philippe Cros ,  Patrice Allibert ,  Fran.cedilla.ois Mallet ,  Claude Mabilat ,  Bernard Mandrand

发明人： Philippe Cros ,  Patrice Allibert ,  Fran.cedilla.ois Mallet ,  Claude Mabilat ,  Bernard Mandrand

IPC分类号： C12Q1/68 ,  C12Q1/70

CPC分类号： C12Q1/6813 ,  C12Q1/6834

摘要： A procedure for detecting single-stranded nucleotide sequences in a sample is disclosed wherein a passively fixed capture probe is used in concert with a non-radioactively labelled detection probe in a sandwich hybridization technique.

摘要翻译： 公开了一种用于检测样品中的单链核苷酸序列的方法，其中在夹心杂交技术中使用被动固定的捕获探针与非放射性标记的检测探针一致。

公开(公告)号：US5656432A

公开(公告)日：1997-08-12

申请号：US486137

申请日：1995-06-07

申请人： Jean-Pierre Claverys ,  Claude Mabilat

发明人： Jean-Pierre Claverys ,  Claude Mabilat

IPC分类号： C12Q1/68

CPC分类号： C12Q1/689

摘要： The invention relates to a fragment of the genomic DNA of Streptococcus pneumoniae, a probe capable of specifically hybridizing with the genomic DNA of Streptococcus pneumoniae, a specific primer for the amplification, by polymerization, of the genomic DNA, a reagent and a method which are used with the probe and, optionally, the primer, for specifically detecting Streptococcus pneumoniae in a biological sample. The probe of the invention is a nucleotide sequence having at least 70% homology with at least a portion of a consensus sequence of the genomic DNA of Streptococcus pneumoniae, this consensus sequence being chosen from the nucleotide sequences SEQ ID NO 2, SEQ ID NO 3, SEQ ID NO 4, which are identified in the description, and their respective complementary sequences.

摘要翻译： 本发明涉及肺炎链球菌的基因组DNA片段，能够与肺炎链球菌的基因组DNA进行特异性杂交的探针，用于扩增，通过聚合的特异性引物，基因组DNA，试剂和方法 与探针一起使用，以及任选的底漆，用于特异性检测生物样品中的肺炎链球菌。 本发明的探针是与肺炎链球菌的基因组DNA的共有序列的至少一部分具有至少70％同源性的核苷酸序列，该共有序列选自核苷酸序列SEQ ID NO：2，SEQ ID NO： ，SEQ ID NO 4，以及它们各自的互补序列。