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11.发明授权Methods of preparing nutritive media for growth and/or germination of plant embryos 有权制备用于植物胚胎生长和/或萌发的营养培养基的方法公开(公告)号：US08871514B2
公开(公告)日：2014-10-28
申请号：US12874089
申请日：2010-09-01
CPC分类号： C12N5/04
摘要： In one aspect, a method is provided for producing an improved nutritive medium comprising an adsorbent material for culturing plant embryos.
摘要翻译： 一方面，提供了一种用于生产包含用于培养植物胚胎的吸附剂材料的改进的营养培养基的方法。
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公开(公告)号：US20130000189A1
公开(公告)日：2013-01-03
申请号：US13533260
申请日：2012-06-26
IPC分类号： A01C1/06
CPC分类号： A01H4/006
摘要： The present disclosure includes a shoot restraint for use with manufactured seeds comprising. In some embodiments, the shoot restraint includes a substantially porous material having a top surface and a bottom surface. The substantially porous material is formed to define a substantially cylindrical upper portion having an upper diameter; and a substantially cylindrical lower portion having a lower diameter. The upper diameter is larger than the lower diameter. A parabolic cavity extends from the top surface to the bottom surface through the upper portion and at least partially into the lower portion.
摘要翻译： 本公开包括与制造的种子一起使用的拍摄约束，包括。 在一些实施例中，拍摄约束包括具有顶表面和底表面的基本上多孔的材料。 形成基本上多孔的材料以限定具有上部直径的基本上圆柱形的上部; 以及具有较小直径的基本上圆柱形的下部。 上直径大于下直径。 抛物面空腔从上表面延伸到底表面，并且至少部分地进入下部。
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公开(公告)号：US07131234B2
公开(公告)日：2006-11-07
申请号：US10982952
申请日：2004-11-04
申请人： William C. Carlson , Jeffrey E. Hartle , Michael K. McKinnis , Curtis A. Bryan , Patrick Gaillard , Edwin Hirahara , Paul G. Gaddis , Katherine M. Salatas
发明人： William C. Carlson , Jeffrey E. Hartle , Michael K. McKinnis , Curtis A. Bryan , Patrick Gaillard , Edwin Hirahara , Paul G. Gaddis , Katherine M. Salatas
摘要： A combination end seal and cotyledon restraint (20) for an artificial seed (22) is provided. The combination end seal and cotyledon restraint includes a cap portion (30) sized to substantially seal one end of an artificial seed and a cotyledon restraint (32) unitarily formed with the cap portion.
摘要翻译： 提供了一种用于人造种子（22）的组合端封和子叶约束（20）。 组合端部密封和子叶约束包括尺寸适于基本上密封人造种子的一端的盖部分（30）和与盖部分整体形成的子叶约束（32）。
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公开(公告)号：US06119395A
公开(公告)日：2000-09-19
申请号：US16827
申请日：1998-01-30
CPC分类号： A01H4/006
摘要： The present invention provides manufactured seeds and related compositions and methods. The manufactured seeds comprise a unit of a totipotent plant tissue enclosed in a protective manufactured seed coat that includes an orifice sealed by a lid. The lid has a burst strength and architecture (e.g., a dome or nipple) similar to those of analogous structures in a natural botanic seed.
摘要翻译： 本发明提供制造的种子及相关的组合物和方法。 所制造的种子包含一个全部植物组织的单位，封闭在保护性制造的种皮中，其包括通过盖子密封的孔口。 盖子具有类似于天然植物种子类似结构的爆裂强度和结构（例如，圆顶或乳头）。
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公开(公告)号：US5486218A
公开(公告)日：1996-01-23
申请号：US69560
申请日：1993-06-01
摘要： An analog of botanic seed is disclosed which comprises a plant embryo preferably encapsulated, or at least in contact with, a hydrated oxygenated gel. The gel can be oxygenated by passing oxygen gas through a gel solution before curing the gel or by exposing the gel to oxygen gas after curing. The gel is preferably oxygenated by adding to an uncured gel solution a suitably stabilized emulsion of a perfluorocarbon compound or a silicone oil, which compounds are capable of absorbing large amounts of oxygen, and are non-toxic and inert. An analog of botanic seed can further comprise an outer shell at least partially surrounding the gel and embryo, thereby forming a capsule. The outer shell preferably is shaped to aid the radicle of a germinating embryo in protrusively rupturing the capsule, thereby facilitating successful germination and minimizing incidence of seedling malformation. Other shell materials are selected to provide requisite rigidity to the capsule while imparting minimal restriction to successful germination.
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公开(公告)号：US08789311B2
公开(公告)日：2014-07-29
申请号：US12243089
申请日：2008-10-01
CPC分类号： A01H4/006
摘要： A manufactured seed is provided. The manufactured seed includes a seed shell housing a nutritive media and a restraint disposed within the seed shell. The restraint includes a cavity. The manufactured seed also includes an embryo disposed within the cavity and a fill material disposed within the cavity. The manufactured further includes a seal disposed on the seed shell to seal the embryo within the seed shell.
摘要翻译： 提供制造的种子。 制造的种子包括容纳营养介质和设置在种子壳内的约束的种子壳。 约束包括一个空腔。 制造的种子还包括设置在空腔内的胚胎和设置在空腔内的填充材料。 制造的还包括设置在种子壳上以密封种子壳内的胚胎的密封件。
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公开(公告)号：US08466086B2
公开(公告)日：2013-06-18
申请号：US10371612
申请日：2003-02-20
申请人： William C. Carlson , Jeffrey E. Hartle , James A. Grob , Katherine M. Salatas , Mollie K. Heilesen
发明人： William C. Carlson , Jeffrey E. Hartle , James A. Grob , Katherine M. Salatas , Mollie K. Heilesen
CPC分类号： A01H4/006
摘要： Manufactured seeds are disclosed that comprise a unit of totipotent plant tissue and a nutritive medium. The nutritive medium can contain a number of different components selected from the following: a gel solute, charcoal, a carbon source, urea, KNO3, NH4NO3, CuCl2, CuSO4, KI, KH2PO4, CaCl2, MgSO4, Na2EDTA, FeSO4, ferric citrate, MnSO4, MnCl2, H3BO3, ZnSO4, CoCl2, Na2MoO4, (NH4)2MoO4, thiamine, riboflavin, pyridoxine, HCl, Ca-pantothenate, nicotinic acid, biotin, folic acid, and myo-inositol. The nutritive medium can also include any of various protein amino acids, any of various polyamines, any of various oxygen-absorbing compounds, any of various non-protein amino acids, and/or a smoke suspension.
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公开(公告)号：US20090090050A1
公开(公告)日：2009-04-09
申请号：US12243089
申请日：2008-10-01
IPC分类号： A01C1/00
CPC分类号： A01H4/006
摘要： A manufactured seed is provided. The manufactured seed includes a seed shell housing a nutritive media and a restraint disposed within the seed shell. The restraint includes a cavity. The manufactured seed also includes an embryo disposed within the cavity and a fill material disposed within the cavity. The manufactured further includes a seal disposed on the seed shell to seal the embryo within the seed shell.
摘要翻译： 提供制造的种子。 制造的种子包括容纳营养介质和设置在种子壳内的约束的种子壳。 约束包括一个空腔。 制造的种子还包括设置在空腔内的胚胎和设置在空腔内的填充材料。 制造的还包括设置在种子壳上以密封种子壳内的胚胎的密封件。
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公开(公告)号：US07168205B2
公开(公告)日：2007-01-30
申请号：US10011540
申请日：2001-12-05
IPC分类号： A01C1/06
摘要： The present invention relates to an improved coating for manufactured seeds wherein the coating is able to resist cracking at temperatures below about 1° C. The coating usable with the present invention is comprised of a wax impregnated cellulose substrate wherein the wax composition is comprised substantially of paraffin hydrocarbon cellulose substrata having a Gaussian distribution of carbon chain length ranging from 21 carbons per chain to 40 carbons per chain and a maximum number of paraffin hydrocarbon chains having 31 carbons per chain. One aspect of the present invention is that it provides a wax formulation able to resist cracking at low temperatures and also a wax formulation with a desirable viscosity for commercial manufactured seed coat applications.
摘要翻译： 本发明涉及一种用于制造种子的改进的涂层，其中该涂层能够在低于约1℃的温度下抵抗裂纹。本发明可用的涂层由蜡浸渍的纤维素基质组成，其中蜡组合物基本上由 石蜡烃纤维素基质具有碳链长度范围从每链21碳到每链40个碳原子的最高数量的链烷烃烃链，每个链具有31个碳的链烷烃链长度。 本发明的一个方面是提供一种能够在低温下耐开裂的蜡配方，还提供了商业制造的种皮涂层应用所需粘度的蜡制剂。
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公开(公告)号：US5666762A
公开(公告)日：1997-09-16
申请号：US487600
申请日：1995-06-07
摘要： The present invention provides manufactured seeds comprising totipotent plant tissue and a hydrated gel, preferably surrounded by a protective seed coat. The cotyledons of the germinating totipotent plant tissue can be enclosed in a shoot restraint that is resistant to penetration by the growing cotyledons. The manufactured seeds can be prepared for long-term storage, e.g., by sparging the hydrated gel with a respiration-limiting gas before, during, or after assembly of the manufactured seed. The seed is then preferably stored in an atmosphere containing levels of a respiration-limiting gas sufficient to keep respiration of the totipotent plant tissue at reduced levels.
摘要翻译： 本发明提供了包含全能植物组织和水合凝胶的制造种子，优选被保护性种皮围绕。 发芽的全能植物组织的子叶可以封闭在能够被生长的子叶渗透的枝条约束条中。 制备的种子可以制备用于长期储存，例如通过在制造的种子组装之前，期间或之后用呼吸限制气体喷射水合凝胶。 然后优选将种子储存在含有足以保持全能植物组织呼吸降低水平的呼吸限制气体含量的气氛中。
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