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公开(公告)号：US07207139B2
公开(公告)日：2007-04-24
申请号：US10981438
申请日：2004-11-03
IPC分类号： A01C1/06
CPC分类号： B65G47/91 , A01C11/00 , B65G2201/02 , B65G2201/0202 , B65G2201/0229
摘要： A vacuum pick-up device 20 includes a device body 22 having a pick-up tip opening 78 to which vacuum pressure is supplied. The vacuum pick-up device 20 further includes a mechanical release assisting device 36 that assists in the release of a grasped object contemporaneously with or slightly subsequent to the cessation of vacuum pressure. A manifold 38 may be included to efficiently and effectively supply vacuum pressure to the device body 22 from a vacuum pressure source.
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公开(公告)号：US07131234B2
公开(公告)日：2006-11-07
申请号：US10982952
申请日：2004-11-04
申请人： William C. Carlson , Jeffrey E. Hartle , Michael K. McKinnis , Curtis A. Bryan , Patrick Gaillard , Edwin Hirahara , Paul G. Gaddis , Katherine M. Salatas
发明人： William C. Carlson , Jeffrey E. Hartle , Michael K. McKinnis , Curtis A. Bryan , Patrick Gaillard , Edwin Hirahara , Paul G. Gaddis , Katherine M. Salatas
摘要： A combination end seal and cotyledon restraint (20) for an artificial seed (22) is provided. The combination end seal and cotyledon restraint includes a cap portion (30) sized to substantially seal one end of an artificial seed and a cotyledon restraint (32) unitarily formed with the cap portion.
摘要翻译： 提供了一种用于人造种子（22）的组合端封和子叶约束（20）。 组合端部密封和子叶约束包括尺寸适于基本上密封人造种子的一端的盖部分（30）和与盖部分整体形成的子叶约束（32）。
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公开(公告)号：US5666762A
公开(公告)日：1997-09-16
申请号：US487600
申请日：1995-06-07
摘要： The present invention provides manufactured seeds comprising totipotent plant tissue and a hydrated gel, preferably surrounded by a protective seed coat. The cotyledons of the germinating totipotent plant tissue can be enclosed in a shoot restraint that is resistant to penetration by the growing cotyledons. The manufactured seeds can be prepared for long-term storage, e.g., by sparging the hydrated gel with a respiration-limiting gas before, during, or after assembly of the manufactured seed. The seed is then preferably stored in an atmosphere containing levels of a respiration-limiting gas sufficient to keep respiration of the totipotent plant tissue at reduced levels.
摘要翻译： 本发明提供了包含全能植物组织和水合凝胶的制造种子，优选被保护性种皮围绕。 发芽的全能植物组织的子叶可以封闭在能够被生长的子叶渗透的枝条约束条中。 制备的种子可以制备用于长期储存，例如通过在制造的种子组装之前，期间或之后用呼吸限制气体喷射水合凝胶。 然后优选将种子储存在含有足以保持全能植物组织呼吸降低水平的呼吸限制气体含量的气氛中。
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公开(公告)号：US5701699A
公开(公告)日：1997-12-30
申请号：US485986
申请日：1995-06-07
摘要： The present invention provides manufactured seeds and related compositions and methods. The manufactured seeds comprise a unit of a totipotent plant tissue, preferably in contact with a hydrated gel. Preferably, the shoot of the germinating embryo is enclosed in a shoot restraint that is resistant to penetration by the growing shoot. The manufactured seed may be at least partially surrounded by a protective manufactured seed coat. The gel or the seed coat can include various additives, such as nutrients, antibiotics, or plant growth regulators.
摘要翻译： 本发明提供制造的种子及相关的组合物和方法。 制造的种子包括全能植物组织的单位，优选与水合凝胶接触。 优选地，发芽胚芽的芽被封闭在能够通过生长的枝条穿透的枝条约束条中。 制造的种子可以至少部分地被保护性制成的种皮包围。 凝胶或种皮可以包括各种添加剂，例如营养物，抗生素或植物生长调节剂。
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公开(公告)号：US5236469A
公开(公告)日：1993-08-17
申请号：US604656
申请日：1990-10-26
摘要： An analog of botanic seed is disclosed which comprises a plant embryo preferably encapsulated, or at least in contact with, a hydrated oxygenated gel. The gel can be oxygenated by passing oxygen gas through a gel solution before curing the gel or by exposing the gel to oxygen gas after curing. The gel is preferably oxygenated by adding to an uncured gel solution a suitably stabilized emulsion of a perfluorocarbon compound or a silicon oil, which compounds are capable of absorbing large amounts of oxygen, and are non-toxic and inert. An analog of botanic see can further comprise an outer shell at least partially surrounding the gel and embryo, thereby forming a capsule. The outer shell preferably is shaped to aid the radicle of a germinating embryo in protrusively rupturing the capsule, thereby facilitating successful germination and minimizing incidence of seedling malformation. Other shell materials are selected to provide requisite rigidity to the capsule while imparting minimal restriction to successful germination.
摘要翻译： 公开了植物种子的类似物，其包含优选包封或至少与水合氧化凝胶接触的植物胚。 在固化凝胶之前，通过使氧气通过凝胶溶液，或者在固化后将凝胶暴露于氧气，可以使凝胶氧化。 优选通过向未固化的凝胶溶液加入适当稳定的全氟化碳化合物或硅油的乳液，该化合物能够吸收大量的氧，并且是无毒的和惰性的，该凝胶是优选的。 植物学类似物可以进一步包含至少部分地围绕凝胶和胚胎的外壳，从而形成胶囊。 外壳优选地被成形为辅助发芽胚胎的胚根突出地破裂胶囊，从而有助于成功发芽并使幼苗畸形的发生率最小化。 选择其它壳材料以为胶囊提供必要的刚性，同时最小限度地限制成功发芽。
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公开(公告)号：US5284765A
公开(公告)日：1994-02-08
申请号：US865390
申请日：1992-04-08
CPC分类号： A01H4/00
摘要： The invention is a method of ordering plant embryos so that a specified end of the embryo is always oriented in a predetermined direction. The embryos are preferably first fairly rapidly desiccated to about 15% moisture content. They are then suspended in a benign liquid flotation medium having a density in the range of about 1.059-1.104 g/cm.sup.3. The density must be adjusted empirically so that a predominant number of viable embryos will float and nonviable embryos will sink. In at least the case of conifer somatic embryos, they will float with the end bearing the latent cotyledons upward. After sufficient separation time in the flotation medium the oriented embryos are swept by a flowing liquid stream into a conduit. They enter cotyledon end first and are then carried to a delivery point without losing that orientation. Here they are separated from the transporting medium. The embryos, still positioned cotyledon end first, may then be picked up by robotic or other means for further processing, such as insertion into an artificial seed.
摘要翻译： 本发明是一种排序植物胚胎的方法，使得胚胎的指定末端总是沿预定方向取向。 胚胎优选首先相当快速地干燥至约15％的水分含量。 然后将它们悬浮在密度在约1.059-1.104g / cm3范围内的良性液体浮选介质中。 密度必须经验性地调整，使得主要数量的活胚可以漂浮，而不可行胚胎将下沉。 至少在针叶树体细胞胚胎的情况下，它们将浮起，其末端将潜在子叶向上。 在浮选介质中足够的分离时间后，定向胚胎被流动的液体流扫入管道中。 它们首先进入子叶，然后携带到一个传送点，而不会失去该方向。 这里它们与运输介质分离。 然后可以通过机器人或其他手段拾取仍然位于子叶的胚胎，用于进一步加工，例如插入人造种子中。
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7.发明授权Methods of preparing nutritive media for growth and/or germination of plant embryos 有权制备用于植物胚胎生长和/或萌发的营养培养基的方法公开(公告)号：US08871514B2
公开(公告)日：2014-10-28
申请号：US12874089
申请日：2010-09-01
CPC分类号： C12N5/04
摘要： In one aspect, a method is provided for producing an improved nutritive medium comprising an adsorbent material for culturing plant embryos.
摘要翻译： 一方面，提供了一种用于生产包含用于培养植物胚胎的吸附剂材料的改进的营养培养基的方法。
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公开(公告)号：US20130000189A1
公开(公告)日：2013-01-03
申请号：US13533260
申请日：2012-06-26
IPC分类号： A01C1/06
CPC分类号： A01H4/006
摘要： The present disclosure includes a shoot restraint for use with manufactured seeds comprising. In some embodiments, the shoot restraint includes a substantially porous material having a top surface and a bottom surface. The substantially porous material is formed to define a substantially cylindrical upper portion having an upper diameter; and a substantially cylindrical lower portion having a lower diameter. The upper diameter is larger than the lower diameter. A parabolic cavity extends from the top surface to the bottom surface through the upper portion and at least partially into the lower portion.
摘要翻译： 本公开包括与制造的种子一起使用的拍摄约束，包括。 在一些实施例中，拍摄约束包括具有顶表面和底表面的基本上多孔的材料。 形成基本上多孔的材料以限定具有上部直径的基本上圆柱形的上部; 以及具有较小直径的基本上圆柱形的下部。 上直径大于下直径。 抛物面空腔从上表面延伸到底表面，并且至少部分地进入下部。
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公开(公告)号：US06119395A
公开(公告)日：2000-09-19
申请号：US16827
申请日：1998-01-30
CPC分类号： A01H4/006
摘要： The present invention provides manufactured seeds and related compositions and methods. The manufactured seeds comprise a unit of a totipotent plant tissue enclosed in a protective manufactured seed coat that includes an orifice sealed by a lid. The lid has a burst strength and architecture (e.g., a dome or nipple) similar to those of analogous structures in a natural botanic seed.
摘要翻译： 本发明提供制造的种子及相关的组合物和方法。 所制造的种子包含一个全部植物组织的单位，封闭在保护性制造的种皮中，其包括通过盖子密封的孔口。 盖子具有类似于天然植物种子类似结构的爆裂强度和结构（例如，圆顶或乳头）。
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公开(公告)号：US5486218A
公开(公告)日：1996-01-23
申请号：US69560
申请日：1993-06-01
摘要： An analog of botanic seed is disclosed which comprises a plant embryo preferably encapsulated, or at least in contact with, a hydrated oxygenated gel. The gel can be oxygenated by passing oxygen gas through a gel solution before curing the gel or by exposing the gel to oxygen gas after curing. The gel is preferably oxygenated by adding to an uncured gel solution a suitably stabilized emulsion of a perfluorocarbon compound or a silicone oil, which compounds are capable of absorbing large amounts of oxygen, and are non-toxic and inert. An analog of botanic seed can further comprise an outer shell at least partially surrounding the gel and embryo, thereby forming a capsule. The outer shell preferably is shaped to aid the radicle of a germinating embryo in protrusively rupturing the capsule, thereby facilitating successful germination and minimizing incidence of seedling malformation. Other shell materials are selected to provide requisite rigidity to the capsule while imparting minimal restriction to successful germination.
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