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公开(公告)号：US20080081371A1
公开(公告)日：2008-04-03
申请号：US11856570
申请日：2007-09-17
Applicant: James A. Grob , Stephanie A. Brusig
Inventor： James A. Grob , Stephanie A. Brusig
IPC: C12N5/02
Abstract: In one aspect, the present invention provides methods of producing conifer cotyledonary somatic embryos from pre-cotyledonary embryos. The methods of this aspect of the invention include the step of (a) dispensing a plurality of pre-cotyledonary embryos onto a porous material horizontally disposed over a non-porous surface in a volume of sterile dilution medium sufficient to submerge at least the surface of the porous material, thereby uniformly dispersing the pre-cotyledonary embryos; (b) removing the sterile dilution medium from the non-absorbent porous material, thereby trapping the uniformly dispersed pre-cotyledonary embryos on the porous material; and (c) contacting the pre-cotyledonary embryos trapped on the porous material with development medium for a period of time sufficient to produce conifer cotyledonary somatic embryos.
Abstract translation: 一方面，本发明提供了从子叶前胚胎产生针叶子子叶体细胞胚的方法。 本发明的这个方面的方法包括以下步骤：（a）将多个子叶前胚胎分配到水平设置在无菌表面上的多孔材料的无菌稀释培养基的体积中，足以浸没至少 多孔材料，从而均匀地分散子叶前胚; （b）从非吸收性多孔材料中除去无菌稀释介质，从而将均匀分散的子叶前胚胎捕获在多孔材料上; 和（c）将捕获在多孔材料上的前子叶胚胎与显影培养基接触足以产生针叶子子叶体细胞胚的时间。
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公开(公告)号：US08466086B2
公开(公告)日：2013-06-18
申请号：US10371612
申请日：2003-02-20
Applicant: William C. Carlson , Jeffrey E. Hartle , James A. Grob , Katherine M. Salatas , Mollie K. Heilesen
Inventor： William C. Carlson , Jeffrey E. Hartle , James A. Grob , Katherine M. Salatas , Mollie K. Heilesen
CPC classification number: A01H4/006
Abstract: Manufactured seeds are disclosed that comprise a unit of totipotent plant tissue and a nutritive medium. The nutritive medium can contain a number of different components selected from the following: a gel solute, charcoal, a carbon source, urea, KNO3, NH4NO3, CuCl2, CuSO4, KI, KH2PO4, CaCl2, MgSO4, Na2EDTA, FeSO4, ferric citrate, MnSO4, MnCl2, H3BO3, ZnSO4, CoCl2, Na2MoO4, (NH4)2MoO4, thiamine, riboflavin, pyridoxine, HCl, Ca-pantothenate, nicotinic acid, biotin, folic acid, and myo-inositol. The nutritive medium can also include any of various protein amino acids, any of various polyamines, any of various oxygen-absorbing compounds, any of various non-protein amino acids, and/or a smoke suspension.
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公开(公告)号：US08061079B2
公开(公告)日：2011-11-22
申请号：US11171157
申请日：2005-06-29
Applicant: William C. Carlson , Christine A. Dean , James A. Grob , George E. Herold , Erin Wallich
Inventor： William C. Carlson , Christine A. Dean , James A. Grob , George E. Herold , Erin Wallich
IPC: A01H3/00
Abstract: A method for selecting conifer trees is disclosed. This method relates to the early measurement of tree characteristics for selecting trees most likely to exhibit certain traits as they mature. This method can be used for implementing silvicultural treatments, thinning or further breeding.
Abstract translation: 公开了一种选择针叶树的方法。 这种方法涉及树木特征的早期测量，用于选择最有可能在成熟时表现出某些特征的树木。 该方法可用于实施造林处理，变薄或进一步育种。
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Patent Agency Ranking
公开(公告)号：US07785884B2
公开(公告)日：2010-08-31
申请号：US11856570
申请日：2007-09-17
Applicant: James A Grob , Stephanie A Brusig
Inventor： James A Grob , Stephanie A Brusig
IPC: C12N5/00
Abstract: In one aspect, the present invention provides methods of producing conifer cotyledonary somatic embryos from pre-cotyledonary embryos. The methods of this aspect of the invention include the step of (a) dispensing a plurality of pre-cotyledonary embryos onto a porous material horizontally disposed over a non-porous surface in a volume of sterile dilution medium sufficient to submerge at least the surface of the porous material, thereby uniformly dispersing the pre-cotyledonary embryos; (b) removing the sterile dilution medium from the non-absorbent porous material, thereby trapping the uniformly dispersed pre-cotyledonary embryos on the porous material; and (c) contacting the pre-cotyledonary embryos trapped on the porous material with development medium for a period of time sufficient to produce conifer cotyledonary somatic embryos.
Abstract translation: 一方面，本发明提供了从子叶前胚胎产生针叶子子叶体细胞胚的方法。 本发明的这个方面的方法包括以下步骤：（a）将多个子叶前胚胎分配到水平设置在无菌表面上的多孔材料的无菌稀释培养基的体积中，足以浸没至少 多孔材料，从而均匀地分散子叶前胚; （b）从非吸收性多孔材料中除去无菌稀释介质，从而将均匀分散的子叶前胚胎捕获在多孔材料上; 和（c）将捕获在多孔材料上的前子叶胚胎与显影培养基接触足以产生针叶子子叶体细胞胚的时间。
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5.发明申请Methods for increasing germination vigor by early singulation of conifer somatic embryos 有权通过早期分选针叶树体细胞胚增加萌发活力的方法公开(公告)号：US20090007303A1
公开(公告)日：2009-01-01
申请号：US12164291
申请日：2008-06-30
Applicant: Roger Timmis , James A. Grob , Pramod K. Gupta , Susan D. Rayfield
Inventor： Roger Timmis , James A. Grob , Pramod K. Gupta , Susan D. Rayfield
IPC: A01H5/00
Abstract: In one aspect, a method is provided for increasing germination vigor of conifer somatic embryos produced in vitro. The method comprises (a) singulating a plurality of individual immature conifer somatic embryos from a first culture of embryos after a first incubation period in a first development media; and (b) contacting the plurality of singulated immature conifer somatic embryos with a second development media for a second incubation period.
Abstract translation: 在一个方面，提供了一种用于增加在体外产生的针叶树体细胞胚的发芽势的方法。 该方法包括：（a）在第一显影培养基中的第一次孵育期后，从第一胚胎培养物中分离多个单个未成熟针叶体细胞胚; 和（b）使多个单切的未成熟针叶树体细胞胚与第二培养基接触第二潜伏期。
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公开(公告)号：US09053353B2
公开(公告)日：2015-06-09
申请号：US11833807
申请日：2007-08-03
Applicant: Roger Timmis , Mitchell R. Toland , Timnit Ghermay , William C. Carlson , James A. Grob
Inventor： Roger Timmis , Mitchell R. Toland , Timnit Ghermay , William C. Carlson , James A. Grob
CPC classification number: G06K9/00147 , A01C1/00 , A01H1/04 , A01H4/00 , B07C2501/009 , C12N15/8207
Abstract: The present invention is directed towards methods for the classification of plant embryos by the application of one or more classification algorithms to analyze digitized images and absorption, transmittance, or reflectance spectra. The methods are generally applicable and emphasize the importance of acquiring and using as much image and absorption, transmittance, or reflectance spectral information as possible, based on objective criteria. The present invention allows automated selection of embryos most suitable for further culture and rejection of those seen as less suitable.
Abstract translation: 本发明涉及通过应用一种或多种分类算法来分析数字化图像和吸收，透射率或反射光谱来分类植物胚胎的方法。 这些方法通常是适用的，并强调基于客观标准获取和使用尽可能多的图像和吸收，透射率或反射光谱信息的重要性。 本发明允许自动选择最适合进一步培养和排除那些被认为不太适合的胚胎。
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7.发明授权Methods for increasing germination vigor by early singulation of conifer somatic embryos 有权通过早期分选针叶树体细胞胚增加萌发活力的方法公开(公告)号：US07964404B2
公开(公告)日：2011-06-21
申请号：US12164291
申请日：2008-06-30
Applicant: Roger Timmis , James A Grob , Pramod K Gupta , Susan D Rayfield
Inventor： Roger Timmis , James A Grob , Pramod K Gupta , Susan D Rayfield
Abstract: In one aspect, a method is provided for increasing germination vigor of conifer somatic embryos produced in vitro. The method comprises (a) singulating a plurality of individual immature conifer somatic embryos from a first culture of embryos after a first incubation period in a first development media; and (b) contacting the plurality of singulated immature conifer somatic embryos with a second development media for a second incubation period.
Abstract translation: 在一个方面，提供了一种用于增加在体外产生的针叶树体细胞胚的发芽势的方法。 该方法包括：（a）在第一显影培养基中的第一次孵育期后，从第一胚胎培养物中分离多个单个未成熟针叶体细胞胚; 和（b）使多个单切的未成熟针叶树体细胞胚与第二培养基接触第二潜伏期。
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公开(公告)号：US06119395A
公开(公告)日：2000-09-19
申请号：US16827
申请日：1998-01-30
Applicant: Jeffrey E. Hartle , William C. Carlson , James A. Grob
Inventor： Jeffrey E. Hartle , William C. Carlson , James A. Grob
CPC classification number: A01H4/006
Abstract: The present invention provides manufactured seeds and related compositions and methods. The manufactured seeds comprise a unit of a totipotent plant tissue enclosed in a protective manufactured seed coat that includes an orifice sealed by a lid. The lid has a burst strength and architecture (e.g., a dome or nipple) similar to those of analogous structures in a natural botanic seed.
Abstract translation: 本发明提供制造的种子及相关的组合物和方法。 所制造的种子包含一个全部植物组织的单位，封闭在保护性制造的种皮中，其包括通过盖子密封的孔口。 盖子具有类似于天然植物种子类似结构的爆裂强度和结构（例如，圆顶或乳头）。
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