公开(公告)号：US20160237452A1

公开(公告)日：2016-08-18

申请号：US15047456

申请日：2016-02-18

Applicant: Dow AgroSciences LLC

Inventor： Thomas Meade ,  Kenneth Narva ,  Nicholas P. Storer ,  Joel J. Sheets ,  Aaron T. Woosley ,  Stephanie L. Burton

IPC: C12N15/82 ,  A01N63/02

CPC classification number: C12N15/8286 ,  A01N63/02 ,  C07K14/325 ,  Y02A40/162

Abstract: The subject invention includes methods and plants for controlling European corn borer, said plants comprising a Cry1Ab insecticidal protein and a DIG-3 insecticidal protein to delay or prevent development of resistance by the insect.

公开(公告)号：US20150335017A1

公开(公告)日：2015-11-26

申请号：US14410762

申请日：2013-06-25

Applicant: DOW AGROSCIENCES LLC

Inventor： Nathan Bard ,  Gregory A. Bradfisch ,  Yunxing Cory Cui ,  James E. Dripps ,  Thomas Hoffman ,  Dayakar Pareddy ,  Dawn M. Parkhurst ,  Sandra G. Toledo ,  Barry Wiggins ,  Ning Zhou ,  Aaron T. Woosley

IPC: A01N43/16 ,  C12N15/82 ,  A01H5/10

CPC classification number: A01N43/16 ,  A01H5/10 ,  C12N15/8274 ,  C12N15/8286 ,  Y02A40/162

Abstract: Soybean event 9582.816.15.1 comprises genes encoding Cry1F, Cry1Ac (synpro), and PAT, affording insect resistance and herbicide tolerance to soybean crops containing the event, and enabling methods for crop protection and protection of stored products. Embodiments of the present disclosure relate to a new insect resistant and herbicide tolerant transgenic soybean transformation event, designated soybean event pDAB9582.816.15.1, comprising cry1F v3 (cry1F), cry1Ac synpro (cry1Ac) and pat v6 (pat), as described herein, inserted into a specific site within the genome of a soybean cell. Representative soybean seed has been deposited with American Type Culture Collection (ATCC) with the Accession No. ATCC Deposit No. PTA-12588. The DNA of soybean plants containing this event includes the junction/flanking sequences described herein that characterize the location of the inserted DNA within the soybean genome.

Abstract translation: 大豆事件9582.816.15.1包含编码Cry1F，Cry1Ac（synpro）和PAT的基因，为含有该事件的大豆作物提供抗昆虫抗性和除草剂耐受性，以及对作物保护和保护储存产品的有效方法。 本公开的实施方案涉及如本文所述的新的耐昆虫抗性和除草剂耐受转基因大豆转化事件，称为大豆事件pDAB9582.816.15.1，包含cry1F v3（cry1F），cry1Ac synpro（cry1Ac）和pat v6（pat） 插入大豆细胞基因组内的特定位点。 代表性的大豆种子已经存入具有登录号ATCC保藏号PTA-12588的美国典型培养物保藏中心（ATCC）。 含有该事件的大豆植物的DNA包括本文所述的连接/侧翼序列，其表征插入的DNA在大豆基因组内的位置。

公开(公告)号：US20140298536A1

公开(公告)日：2014-10-02

申请号：US14105231

申请日：2013-12-13

Applicant: DOW AGROSCIENCES LLC

Inventor： Kenneth Narva ,  Huarong Li ,  Chaoxian Geng ,  Ignacio M. Larrinua ,  Monica B. Olson ,  Navin Elango ,  Matthew J. Henry ,  Aaron T. Woosley ,  Murugesan Rangasamy

IPC: C12N15/113 ,  C12N15/82

CPC classification number: C12N15/113 ,  C12N15/8286 ,  C12N2310/14 ,  Y02A40/162

Abstract: This disclosure concerns nucleic acid molecules and methods of use thereof for control of coleopteran pests through RNA interference-mediated inhibition of target coding and transcribed non-coding sequences in coleopteran pests. The disclosure also concerns methods for making transgenic plants that express nucleic acid molecules useful for the control of coleopteran pests, and the plant cells and plants obtained thereby.

Abstract translation: 本公开涉及核酸分子及其用于通过RNA干扰介导的鞘内鳞翅目害虫中目标编码和转录的非编码序列的抑制来控制鞘翅目害虫的方法。 本公开还涉及用于制备表达可用于控制鞘翅目害虫的核酸分子的转基因植物以及由此获得的植物细胞和植物的方法。

公开(公告)号：US20210277413A1

公开(公告)日：2021-09-09

申请号：US15770379

申请日：2016-10-20

Applicant: Dow AgroSciences LLC

Inventor： Kenneth E. Narva ,  Huarong Li ,  Chaoxian Geng ,  Ignacio Mario Larrinua ,  Navin Elango ,  Aaron T. Woosley ,  Monica B. Olson ,  Matthew J. Henry ,  Murugesan Rangasamy ,  Kanika Arora ,  Premchand Gandra ,  Sarah E. Worden

IPC: C12N15/82 ,  C07K14/435

Abstract: This disclosure concerns nucleic acid molecules and methods of use thereof for control of coleopteran pests through RNA interference-mediated inhibition of target coding and transcribed non-coding sequences in coleopteran pests. The disclosure also concerns methods for making transgenic plants that express nucleic acid molecules useful for the control of coleopteran pests, and the plant cells and plants obtained thereby.

公开(公告)号：US20200224214A1

公开(公告)日：2020-07-16

申请号：US15757997

申请日：2015-10-07

Applicant: Dow AgroSciences LLC

Inventor： Kenneth E. Narva ,  Huarong Li ,  Chaoxian Geng ,  Navin Elango ,  Marrhew J, Henry ,  Murugesan Rangasamy ,  Aaron T. Woosley ,  Kanika Arora ,  Premchand Gandra ,  Sarah E. Worden ,  Elane Fishilevich

IPC: C12N15/82 ,  C07K14/435

Abstract: This disclosure concerns nucleic acid molecules and methods of use thereof for control of insect pests through RNA interference-mediated inhibition of target coding and transcribed non-coding sequences in insect pests, including coleopteran and/or hemipteran pests. The disclosure also concerns methods for making transgenic plants that express nucleic acid molecules useful for the control of insect pests, and the plant cells and plants obtained thereby.

公开(公告)号：US10119149B2

公开(公告)日：2018-11-06

申请号：US15047456

申请日：2016-02-18

Applicant: Dow AgroSciences LLC

Inventor： Thomas Meade ,  Kenneth Narva ,  Nicholas P. Storer ,  Joel J. Sheets ,  Aaron T. Woosley ,  Stephanie L. Burton

IPC: C12N15/82 ,  A01H5/10 ,  C07K14/325 ,  C12N15/32 ,  A01N63/02

Abstract: The subject invention includes methods and plants for controlling European corn borer, said plants comprising a Cry1Ab insecticidal protein and a DIG-3 insecticidal protein to delay or prevent development of resistance by the insect.

公开(公告)号：US10028510B2

公开(公告)日：2018-07-24

申请号：US14831301

申请日：2015-08-20

Applicant: Dow AgroSciences LLC

Inventor： Justin M. Lira ,  Holly Jean Butler ,  Timothy D. Hey ,  Doug A. Smith ,  Kenneth Narva ,  Aaron T. Woosley

IPC: C12N15/82 ,  C12N15/32 ,  C07K14/325 ,  A01N63/02 ,  A01N37/46

Abstract: DIG-17 insecticidal toxins, polynucleotides encoding such toxins, use of such toxins to control pests, and transgenic plants that produce such toxins are disclosed.

公开(公告)号：US09534218B2

公开(公告)日：2017-01-03

申请号：US14409416

申请日：2013-06-13

Applicant: Dow AgroSciences LLC

Inventor： Matthew Kelker ,  Aaron T. Woosley

IPC: C12N15/64 ,  C12N15/11 ,  C12N15/63 ,  C12N15/78 ,  C12P21/00 ,  C12N1/21 ,  C12N15/67

CPC classification number: C12N15/11 ,  C12N15/63 ,  C12N15/64 ,  C12N15/78 ,  C12N2310/353 ,  C12P21/00

Abstract: Compositions and methods for improving expression of a recombinant protein or polypeptide of interest in a host cell are provided. Compositions comprising a polynucleotide coding sequence for a BTBooster are provided using a plant optimized system. The coding sequences can be used in vector constructs or expression systems for transformation and expression of a recombinant protein or polypeptide of interest in a host cell. Methods comprising the codon optimization of a polynucleotide coding sequence for increased expression in a bacterial host are provided. The codon optimization method can be used in designing a polynucleotide coding sequence which expresses robust levels of protein in a bacterial host cell.

Abstract translation: 提供了用于改善宿主细胞中目的重组蛋白或多肽表达的组合物和方法。 使用植物优化系统提供包含BTBooster的多核苷酸编码序列的组合物。 编码序列可用于载体构建体或表达系统，用于在宿主细胞中转化和表达目标重组蛋白或多肽。 提供了包括用于在细菌宿主中增加表达的多核苷酸编码序列的密码子优化的方法。 密码子优化方法可用于设计在细菌宿主细胞中表达强大的蛋白质水平的多核苷酸编码序列。

公开(公告)号：US09487798B2

公开(公告)日：2016-11-08

申请号：US13897082

申请日：2013-05-17

Applicant: Dow AgroSciences LLC

Inventor： Justin M. Lira ,  Kenneth Narva ,  Aaron T. Woosley ,  Ignacio M. Larrinua ,  Timothy D. Hey

IPC: C12N15/82 ,  C07K14/325

CPC classification number: C12N15/8286 ,  C07K14/325 ,  Y02A40/162

Abstract: DIG-10 Cry toxins, polynucleotides encoding such toxins, use of such toxins to control pests, and transgenic plants that produce such toxins are disclosed.

Abstract translation: 公开了DIG-10 Cry毒素，编码这种毒素的多核苷酸，使用这些毒素来控制害虫，以及产生这种毒素的转基因植物。