公开(公告)号：US10577616B2

公开(公告)日：2020-03-03

申请号：US15943106

申请日：2018-04-02

Applicant: Dow AgroSciences LLC ,  Sangamo Therapeutics, Inc.

Inventor： Noel Cogan ,  John Forster ,  Matthew Hayden ,  Tim Sawbridge ,  German Spangenberg ,  Steven R. Webb ,  Manju Gupta ,  W. Michael Ainley ,  Matthew J. Henry ,  Jeffrey C. Miller ,  Dmitry Y. Guschin

IPC: C12N15/82 ,  C12N15/90

Abstract: A method of gene editing or gene stacking within a FAD2 loci by cleaving, in a site directed manner, a location in a FAD2 gene in a cell, to generate a break in the FAD2 gene and then ligating into the break a nucleic acid molecule associated with one or more traits of interest is disclosed.

公开(公告)号：US20200087671A1

公开(公告)日：2020-03-19

申请号：US16698077

申请日：2019-11-27

Applicant: Dow AgroSciences LLC ,  Sangamo Therapeutics, Inc.

Inventor： Noel Cogan ,  John Forster ,  Matthew Hayden ,  Tim Sawbridge ,  German Spangenberg ,  Steven R. Webb ,  Manju Gupta ,  W. Michael Ainley ,  Matthew J. Henry ,  Jeffrey C. Miller ,  Dmitry Y. Guschin

IPC: C12N15/79 ,  C12N15/82 ,  C12N15/63 ,  C12N9/22

Abstract: A method of gene editing or gene stacking within a FAD3 loci by cleaving, in a site directed manner, a location in a FAD3 gene in a cell, to generate a break in the FAD3 gene and then ligating into the break a nucleic acid molecule associated with one or more traits of interest is disclosed.

公开(公告)号：US20180223309A1

公开(公告)日：2018-08-09

申请号：US15757988

申请日：2015-10-07

Applicant: Dow AgroSciences LLC

Inventor： Kenneth E. Narva ,  Huarong Li ,  Chaoxian Geng ,  Navin Elango ,  Matthew J. Henry ,  Murugesan Rangasamy ,  Aaron T. Woosley ,  Kanika Arora ,  Premchand Gandra ,  Sarah E. Worden ,  Elane Fishilevich

IPC: C12N15/82 ,  C12N15/113

CPC classification number: C12N15/8286 ,  C12N15/113 ,  C12N15/8218 ,  C12N2310/14 ,  Y02A40/162 ,  C12N2310/531

Abstract: This disclosure concerns nucleic acid molecules and methods of use thereof for control of insect pests through RNA interference-mediated inhibition of target coding and transcribed non-coding sequences in insect pests, including coleopteran and/or hemipteran pests. The disclosure also concerns methods for making transgenic plants that express nucleic acid molecules useful for the control of insect pests, and the plant cells and plants obtained thereby.

公开(公告)号：US20210277413A1

公开(公告)日：2021-09-09

申请号：US15770379

申请日：2016-10-20

Applicant: Dow AgroSciences LLC

Inventor： Kenneth E. Narva ,  Huarong Li ,  Chaoxian Geng ,  Ignacio Mario Larrinua ,  Navin Elango ,  Aaron T. Woosley ,  Monica B. Olson ,  Matthew J. Henry ,  Murugesan Rangasamy ,  Kanika Arora ,  Premchand Gandra ,  Sarah E. Worden

IPC: C12N15/82 ,  C07K14/435

Abstract: This disclosure concerns nucleic acid molecules and methods of use thereof for control of coleopteran pests through RNA interference-mediated inhibition of target coding and transcribed non-coding sequences in coleopteran pests. The disclosure also concerns methods for making transgenic plants that express nucleic acid molecules useful for the control of coleopteran pests, and the plant cells and plants obtained thereby.

公开(公告)号：US20150067921A1

公开(公告)日：2015-03-05

申请号：US14019211

申请日：2013-09-05

Applicant: Sangamo BioSciences, Inc. ,  Dow AgroSciences LLC

Inventor： Noel Cogan ,  John Forster ,  Matthew Hayden ,  Tim Sawbridge ,  German Spangenberg ,  Steven R. Webb ,  Manju Gupta ,  William Michael Ainley ,  Matthew J. Henry ,  Jeffrey C. Miller ,  Dmitry Y. Guschin

IPC: C12N15/79 ,  C12N15/82 ,  C12N9/22 ,  C12N15/63

CPC classification number: C12N15/79 ,  C12N9/22 ,  C12N15/63 ,  C12N15/8241 ,  C12N15/8247

Abstract: A method of gene editing or gene stacking within a FAD3 loci by cleaving, in a site directed manner, a location in a FAD3 gene in a cell, to generate a break in the FAD3 gene and then ligating into the break a nucleic acid molecule associated with one or more traits of interest is disclosed.

Abstract translation: 通过以位点方式切割细胞中FAD3基因的位置，在FAD3基因座中进行基因编辑或基因堆积的方法，以在FAD3基因中产生断裂，然后连接到断裂中，与核酸分子缔合 公开了一种或多种感兴趣的特征。

公开(公告)号：US10526610B2

公开(公告)日：2020-01-07

申请号：US15882609

申请日：2018-01-29

Applicant: Dow AgroSciences LLC ,  Sangamo Therapeutics, Inc.

Inventor： Noel Cogan ,  John Forster ,  Matthew Hayden ,  Tim Sawbridge ,  German Spangenberg ,  Steven R. Webb ,  Manju Gupta ,  W. Michael Ainley ,  Matthew J. Henry ,  Jeffrey C. Miller ,  Dmitry Y. Guschin

IPC: C12N15/79 ,  C12N15/82 ,  C12N9/22 ,  C12N15/63

Abstract: A method of gene editing or gene stacking within a FAD3 loci by cleaving, in a site directed manner, a location in a FAD3 gene in a cell, to generate a break in the FAD3 gene and then ligating into the break a nucleic acid molecule associated with one or more traits of interest is disclosed.

公开(公告)号：US20180163217A1

公开(公告)日：2018-06-14

申请号：US15882609

申请日：2018-01-29

Applicant: Dow AgroSciences LLC ,  Sangamo Therapeutics, Inc.

Inventor： Noel Cogan ,  John Forster ,  Matthew Hayden ,  Tim Sawbridge ,  German Spangenberg ,  Steven R. Webb ,  Manju Gupta ,  W. Michael Ainley ,  Matthew J. Henry ,  Jeffrey C. Miller ,  Dmitry Y. Guschin

IPC: C12N15/79 ,  C12N9/22 ,  C12N15/82 ,  C12N15/63

CPC classification number: C12N15/79 ,  C12N9/22 ,  C12N15/63 ,  C12N15/8241 ,  C12N15/8247

Abstract: A method of gene editing or gene stacking within a FAD3 loci by cleaving, in a site directed manner, a location in a FAD3 gene in a cell, to generate a break in the FAD3 gene and then ligating into the break a nucleic acid molecule associated with one or more traits of interest is disclosed.

公开(公告)号：US20140298536A1

公开(公告)日：2014-10-02

申请号：US14105231

申请日：2013-12-13

Applicant: DOW AGROSCIENCES LLC

Inventor： Kenneth Narva ,  Huarong Li ,  Chaoxian Geng ,  Ignacio M. Larrinua ,  Monica B. Olson ,  Navin Elango ,  Matthew J. Henry ,  Aaron T. Woosley ,  Murugesan Rangasamy

IPC: C12N15/113 ,  C12N15/82

CPC classification number: C12N15/113 ,  C12N15/8286 ,  C12N2310/14 ,  Y02A40/162

Abstract: This disclosure concerns nucleic acid molecules and methods of use thereof for control of coleopteran pests through RNA interference-mediated inhibition of target coding and transcribed non-coding sequences in coleopteran pests. The disclosure also concerns methods for making transgenic plants that express nucleic acid molecules useful for the control of coleopteran pests, and the plant cells and plants obtained thereby.

Abstract translation: 本公开涉及核酸分子及其用于通过RNA干扰介导的鞘内鳞翅目害虫中目标编码和转录的非编码序列的抑制来控制鞘翅目害虫的方法。 本公开还涉及用于制备表达可用于控制鞘翅目害虫的核酸分子的转基因植物以及由此获得的植物细胞和植物的方法。

公开(公告)号：US20140090113A1

公开(公告)日：2014-03-27

申请号：US14020575

申请日：2013-09-06

Applicant: Dow AgroSciences LLC

Inventor： Noel Cogan ,  John Forster ,  Matthew Hayden ,  Tim Sawbridge ,  German Spangenberg ,  Steven R. Webb ,  Manju Gupta ,  W. Mike Ainley ,  Matthew J. Henry ,  John Mason ,  Sandeep Kumar ,  Stephen Novak

IPC: C12N15/82

Abstract: An Engineered Transgene Integration Platform (ETIP) is described that can be inserted randomly or at targeted locations in plant genomes to facilitate rapid selection and detection of a GOI that is perfectly targeted (both the 5′ and 3′ ends) at the ETIP genomic location. One element in the subject disclosure is the introduction of specific double stranded breaks within the ETIP. In some embodiments, an ETIP is described using zinc finger nuclease binding sites, but may utilize other targeting technologies such as meganucleases, CRISPRs, TALs, or leucine zippers. Also described are compositions of, and methods for producing, transgenic plants wherein the donor or payload DNA expresses one or more products of an exogenous nucleic acid sequence (e.g. protein or RNA) that has been stably-integrated into an ETIP in a plant cell. In embodiments, the ETIP facilitates testing of gene candidates and plant expression vectors from ideation through Development phases.

Abstract translation: 描述了可以随机或在植物基因组中的目标位置插入工程化转基因整合平台（ETIP），以便于在ETIP基因组位置上完全靶向（5'和3'末端）的GOI的快速选择和检测 。 主题披露中的一个要素是在ETIP内引入特定的双链断裂。 在一些实施方案中，使用锌指核酸酶结合位点描述ETIP，但可以利用其它靶向技术，例如大范围核酸酶，CRISPR，TAL或亮氨酸拉链。 还描述了转基因植物的组合物和生产方法，其中供体或有效载体DNA表达已经稳定整合到植物细胞中的ETIP中的外源核酸序列（例如蛋白质或RNA）的一种或多种产物。 在实施方案中，ETIP有助于从构想到开发阶段测试基因候选物和植物表达载体。

公开(公告)号：US10961540B2

公开(公告)日：2021-03-30

申请号：US16698077

申请日：2019-11-27

Applicant: Dow AgroSciences LLC ,  Sangamo Therapeutics, Inc.

Inventor： Noel Cogan ,  John Forster ,  Matthew Hayden ,  Tim Sawbridge ,  German Spangenberg ,  Steven R. Webb ,  Manju Gupta ,  W. Michael Ainley ,  Matthew J. Henry ,  Jeffrey C. Miller ,  Dmitry Y. Guschin

IPC: C12N15/82 ,  C12N9/22 ,  C12N15/63 ,  C12N15/79

Abstract: A method of gene editing or gene stacking within a FAD3 loci by cleaving, in a site directed manner, a location in a FAD3 gene in a cell, to generate a break in the FAD3 gene and then ligating into the break a nucleic acid molecule associated with one or more traits of interest is disclosed.