公开(公告)号：US10455834B2

公开(公告)日：2019-10-29

申请号：US14410762

申请日：2013-06-25

Applicant: DOW AGROSCIENCES LLC

Inventor： Nathan Bard ,  Gregory A. Bradfisch ,  Yunxing Cory Cui ,  James E. Dripps ,  Thomas Hoffman ,  Dayakar Pareddy ,  Dawn M. Parkhurst ,  Sandra G. Toledo ,  Barry Wiggins ,  Ning Zhou ,  Aaron T. Woosley

IPC: C12N15/82 ,  A01N43/16 ,  A01H5/10

Abstract: Soybean event 9582.816.15.1 comprises genes encoding Cry1 F, Cry1 Ac (synpro), and PAT, affording insect resistance and herbicide tolerance to soybean crops containing the event, and enabling methods for crop protection and protection of stored products. Embodiments of the present disclosure relate to a new insect resistant and herbicide tolerant transgenic soybean transformation event, designated soybean event pDAB9582.816.15.1, comprising cry1 F v3 (cry1 F), cry1 Ac synpro (cry1 Ac) and pat v6 (pat), as described herein, inserted into a specific site within the genome of a soybean cell. Representative soybean seed has been deposited with American Type Culture Collection (ATCC) with the Accession No. ATCC Deposit No. PTA-12588. The DNA of soybean plants containing this event includes the junction/flanking sequences described herein that characterize the location of the inserted DNA within the soybean genome.

公开(公告)号：US20160304900A1

公开(公告)日：2016-10-20

申请号：US15194925

申请日：2016-06-28

Applicant: Dow AgroSciences LLC

Inventor： Ignacio M. Larrinua ,  Donald Merlo ,  Avutu Sambi Reddy ,  Arvind Kumar ThirumalaiswamySekhar ,  Aaron T. Woosley

IPC: C12N15/82 ,  C07K14/325 ,  C07K14/37 ,  C07K14/38 ,  C07K14/415 ,  C07K14/435 ,  C12N9/02 ,  C07K14/195

CPC classification number: C12N15/8286 ,  A23D9/00 ,  A23L7/10 ,  A23L7/198 ,  A23L11/00 ,  A23L11/05 ,  A23L19/10 ,  C07K14/195 ,  C07K14/325 ,  C07K14/37 ,  C07K14/38 ,  C07K14/415 ,  C07K14/43595 ,  C11B1/00 ,  C12N9/0065 ,  C12N9/0069 ,  C12N9/0083 ,  C12Y111/01015 ,  C12Y113/00 ,  Y02A40/162

Abstract: The invention provides synthetic nucleic acid sequences encoding proteins of interest that are particularly adapted to express well in plants. The claimed synthetic sequences utilize plant-optimized codons roughly in the same frequency at which they are utilized, on average, in genes naturally occurring in the plant species. The invention further includes synthetic DNA sequence for herbicide tolerance, water and/or heat stress tolerance, healthy oil modifications and for transformation marker genes and selectable marker genes are used. DNA construct and transgenic plants containing the synthetic sequences are taught as are methods and compositions for using the plants in agriculture.

Abstract translation: 本发明提供编码感兴趣的蛋白质的合成核酸序列，其特别适于在植物中表达良好。 所要求的合成序列利用植物优化的密码子，其平均来说在天然存在于植物物种中的基因中使用大致相同的频率。 本发明还包括用于除草剂耐受性，水和/或热胁迫耐受性，健康油改性和用于转化标记基因和选择标记基因的合成DNA序列。 教导含有合成序列的DNA构建体和转基因植物，以及在农业中使用植物的方法和组合物。

公开(公告)号：US20160058017A1

公开(公告)日：2016-03-03

申请号：US14831301

申请日：2015-08-20

Applicant: Dow AgroSciences LLC

Inventor： Justin M. Lira ,  Holly Jean Butler ,  Timothy D. Hey ,  Doug A. Smith ,  Kenneth Narva ,  Aaron T. Woosley

IPC: A01N63/02 ,  C07K14/325

CPC classification number: A01N63/02 ,  A01N37/46 ,  C07K14/325 ,  C07K2319/55 ,  C12N15/8286

Abstract: DIG-17 insecticidal toxins, polynucleotides encoding such toxins, use of such toxins to control pests, and transgenic plants that produce such toxins are disclosed.

Abstract translation: 公开了DIG-17杀虫毒素，编码这种毒素的多核苷酸，使用这些毒素来控制害虫，以及产生这种毒素的转基因植物。

公开(公告)号：US20190203224A1

公开(公告)日：2019-07-04

申请号：US16354778

申请日：2019-03-15

Applicant: DOW AGROSCIENCES LLC

Inventor： Kenneth E. Narva ,  Thomas Meade ,  Kristin J. Fencil ,  Huarong Li ,  Timothy D. Hey ,  Aaron T. Woosley ,  Monica Britt Olson

IPC: C12N15/82 ,  A01G22/00 ,  A01N43/50 ,  B65D85/00 ,  B32B1/02 ,  C07K14/325 ,  A01N37/18 ,  A01H5/10

CPC classification number: C12N15/8286 ,  A01G22/00 ,  A01H5/10 ,  A01N37/18 ,  A01N43/50 ,  B32B1/02 ,  B65D85/00 ,  C07K14/325 ,  Y02A40/162 ,  Y10T428/13

Abstract: The subject invention relates in part to Cry34Ab/35Ab in combination with Cry3Aa. The subject invention relates in part to the surprising discovery that combinations of Cry34Ab/Cry35Ab and Cry3Aa are useful for preventing development of resistance (to either insecticidal protein system alone) by a corn rootworm (Diabrotica spp.) population. As one skilled in the art will recognize with the benefit of this disclosure, corn plants producing these insecticidal Cry proteins will be useful to mitigate concern that a corn rootworm population could develop that would be resistant to either of these insecticidal protein systems alone. Plants (and acreage planted with such plants) that produce these two insecticidal protein systems are included within the scope of the subject invention.

公开(公告)号：US20180223309A1

公开(公告)日：2018-08-09

申请号：US15757988

申请日：2015-10-07

Applicant: Dow AgroSciences LLC

Inventor： Kenneth E. Narva ,  Huarong Li ,  Chaoxian Geng ,  Navin Elango ,  Matthew J. Henry ,  Murugesan Rangasamy ,  Aaron T. Woosley ,  Kanika Arora ,  Premchand Gandra ,  Sarah E. Worden ,  Elane Fishilevich

IPC: C12N15/82 ,  C12N15/113

CPC classification number: C12N15/8286 ,  C12N15/113 ,  C12N15/8218 ,  C12N2310/14 ,  Y02A40/162 ,  C12N2310/531

Abstract: This disclosure concerns nucleic acid molecules and methods of use thereof for control of insect pests through RNA interference-mediated inhibition of target coding and transcribed non-coding sequences in insect pests, including coleopteran and/or hemipteran pests. The disclosure also concerns methods for making transgenic plants that express nucleic acid molecules useful for the control of insect pests, and the plant cells and plants obtained thereby.

公开(公告)号：US20160060306A1

公开(公告)日：2016-03-03

申请号：US14830997

申请日：2015-08-20

Applicant: DOW AGROSCIENCES LLC

Inventor： Justin M. Lira ,  Holly Jean Butler ,  Doug A. Smith ,  Kenneth Narva ,  Aaron T. Woosley

IPC: C07K14/325 ,  A01N37/18 ,  C12N15/82

CPC classification number: C07K14/325 ,  A01N37/46 ,  A01N63/02 ,  C12N15/8286 ,  Y02A40/162

Abstract: DIG-14 insecticidal toxins, polynucleotides encoding such toxins, use of such toxins to control pests, and transgenic plants that produce such toxins are disclosed.

Abstract translation: 公开了DIG-14杀虫毒素，编码这种毒素的多核苷酸，使用这些毒素来控制害虫，以及产生这种毒素的转基因植物。

公开(公告)号：US20180346926A1

公开(公告)日：2018-12-06

申请号：US16049169

申请日：2018-07-30

Applicant: DOW AGROSCIENCES LLC

Inventor： Ignacio Mario Larrinua ,  Donald J. Merlo ,  Avutu S. Reddy ,  Arvind Kumar ThirumalaiswamySekhar ,  Aaron T. Woosley

IPC: C12N15/82 ,  A23D9/00 ,  C07K14/415 ,  C12N9/02 ,  C07K14/195 ,  C07K14/37 ,  C07K14/38 ,  A23L19/10 ,  A23L11/00 ,  A23L7/10 ,  C12N9/08 ,  C11B1/00 ,  C07K14/435 ,  C07K14/325

CPC classification number: C12N15/8286 ,  A23D9/00 ,  A23L7/10 ,  A23L7/198 ,  A23L11/00 ,  A23L11/05 ,  A23L19/10 ,  C07K14/195 ,  C07K14/325 ,  C07K14/37 ,  C07K14/38 ,  C07K14/415 ,  C07K14/43595 ,  C11B1/00 ,  C12N9/0065 ,  C12N9/0069 ,  C12N9/0083 ,  C12Y111/01015 ,  C12Y113/00 ,  Y02A40/162

Abstract: The invention provides synthetic nucleic acid sequences encoding proteins of interest that are particularly adapted to express well in plants. The claimed synthetic sequences utilize plant-optimized codons roughly in the same frequency at which they are utilized, on average, in genes naturally occurring in the plant species. The invention further includes synthetic DNA sequence for herbicide tolerance, water and/or heat stress tolerance, healthy oil modifications and for transformation marker genes and selectable marker genes are used. DNA construct and transgenic plants containing the synthetic sequences are taught as are methods and compositions for using the plants in agriculture.

公开(公告)号：US09688983B2

公开(公告)日：2017-06-27

申请号：US14105231

申请日：2013-12-13

Applicant: Dow AgroSciences LLC

Inventor： Kenneth Narva ,  Huarong Li ,  Chaoxian Geng ,  Ignacio M. Larrinua ,  Monica B. Olson ,  Navin Elango ,  Matthew J. Henry ,  Aaron T. Woosley ,  Murugesan Rangasamy

IPC: C12N15/11 ,  C12N15/82 ,  C12N15/113

CPC classification number: C12N15/113 ,  C12N15/8286 ,  C12N2310/14 ,  Y02A40/162

Abstract: This disclosure concerns nucleic acid molecules and methods of use thereof for control of coleopteran pests through RNA interference-mediated inhibition of target coding and transcribed non-coding sequences in coleopteran pests. The disclosure also concerns methods for making transgenic plants that express nucleic acid molecules useful for the control of coleopteran pests, and the plant cells and plants obtained thereby.

公开(公告)号：US20150191721A1

公开(公告)日：2015-07-09

申请号：US14409416

申请日：2013-06-13

Applicant: Dow AgroSciences LLC

Inventor： Matthew Kelker ,  Aaron T. Woosley

IPC: C12N15/11 ,  C12P21/00 ,  C12N15/64

CPC classification number: C12N15/11 ,  C12N15/63 ,  C12N15/64 ,  C12N15/78 ,  C12N2310/353 ,  C12P21/00

Abstract: Compositions and methods for improving expression of a recombinant protein or polypeptide of interest in a host cell are provided. Compositions comprising a polynucleotide coding sequence for a BTBooster are provided using a plant optimized system. The coding sequences can be used in vector constructs or expression systems for transformation and expression of a recombinant protein or polypeptide of interest in a host cell. Methods comprising the codon optimization of a polynucleotide coding sequence for increased expression in a bacterial host are provided. The codon optimization method can be used in designing a polynucleotide coding sequence which expresses robust levels of protein in a bacterial host cell.

Abstract translation: 提供了用于改善宿主细胞中目的重组蛋白或多肽表达的组合物和方法。 使用植物优化系统提供包含BTBooster的多核苷酸编码序列的组合物。 编码序列可用于载体构建体或表达系统，用于在宿主细胞中转化和表达目标重组蛋白或多肽。 提供了包括用于在细菌宿主中增加表达的多核苷酸编码序列的密码子优化的方法。 密码子优化方法可用于设计在细菌宿主细胞中表达强大的蛋白质水平的多核苷酸编码序列。

公开(公告)号：US20130247254A1

公开(公告)日：2013-09-19

申请号：US13897082

申请日：2013-05-17

Applicant: DOW AGROSCIENCES LLC

Inventor： Justin M. Lira ,  Kenneth Narva ,  Aaron T. Woosley ,  Ignacio M. Larrinua ,  Timothy D. Hey

IPC: C12N15/82

CPC classification number: C12N15/8286 ,  C07K14/325 ,  Y02A40/162

Abstract: DIG-10 Cry toxins, polynucleotides encoding such toxins, use of such toxins to control pests, and transgenic plants that produce such toxins are disclosed.

Abstract translation: 公开了DIG-10 Cry毒素，编码这种毒素的多核苷酸，使用这些毒素来控制害虫，以及产生这种毒素的转基因植物。